LOCUS       MR211254                7695 bp    DNA     circular  SYN 
DEFINITION  Ap2a2 (Myc-DDK-tagged) - Mouse adaptor protein complex AP-2, alpha 2 subunit (Ap2a2)
ACCESSION   NM_007459
VERSION     NM_007459.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3852..3943 
                     /label="Myc-DDK"  
     misc_feature    3973..4558 
                     /label="PolyA signal"  
     misc_feature    4030..4049 
                     /label="Restriction Enzyme"  
     misc_feature    4834..5506 
                     /label="ColE1"  
     misc_feature    5936..6730 
                     /label="Kan/Neo"  
     misc_feature    6765..7067 
                     /label="SV40 ori"  
     misc_feature    7153..7630 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3834..3840 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3834 
                     /label="Ap2a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCCGTA TCCAAGGGGG ACGGGATGCG AGGCCTAGCG GTCTTCATCT 
     1081 CCGACATCCG CAACTGTAAA AGTAAAGAAG CTGAAATAAA GAGAATAAAC AAAGAACTGG 
     1141 CAAATATTAG ATCCAAATTT AAAGGTGACA ACGCTCTTGA TGGCTACAGT AAAAAGAAGT 
     1201 ATGTCTGCAA ATTGCTCTTC ATCTTTCTCC TTGGTCATGA CATTGACTTT GGACACATGG 
     1261 AAGCTGTGAA TCTTCTGAGC TCAAACAGAT ACACGGAAAA GCAGATTGGC TACCTTTTCA 
     1321 TCTCTGTATT GGTGAACTCG AATAGTGAAC TGATCCGGTT GATTAACAAT GCCATCAAGA 
     1381 ATGACCTGGC CAGCCGCAAT CCTACGTTTA TGGGTCTCGC CCTGCACTGC ATTGCCAATG 
     1441 TGGGTAGCCG TGAGATGGCG GAGGCCTTCG CCGGAGAGAT CCCCAAGATC CTCGTGGCAG 
     1501 GAGATACCAT GGATAGTGTG AAGCAGAGCG CAGCCCTGTG CTTGTTACGC CTGTACAGGA 
     1561 CATCACCTGA CCTAGTTCCC ATGGGCGACT GGACATCTCG TGTGGTACAC CTCCTCAATG 
     1621 ACCAGCACTT GGGGGTAGTG ACTGCAGCGA CTAGCCTCAT CACCACCCTG GCCCAGAAGA 
     1681 ACCCTGAGGA GTTCAAGACC TCTGTCTCGC TAGCTGTCTC GCGACTGAGC AGGATTGTGA 
     1741 CGTCTGCATC AACAGATCTT CAGGATTATA CTTACTACTT TGTCCCGGCT CCTTGGCTGT 
     1801 CAGTCAAGCT TCTGAGACTG CTGCAGTGCT ACCCACCCCC AGACCCTGCG GTGCGTGGCC 
     1861 GCCTGACTGA GTGCTTGGAG ACCATCCTGA ACAAGGCCCA GGAGCCACCC AAGTCCAAGA 
     1921 AGGTCCAGCA TTCAAATGCC AAGAACGCTG TGCTGTTTGA GGCCATCAGC CTGATCATCC 
     1981 ACCATGACAG TGAGCCAAAC TTGCTCGTCC GCGCCTGCAA CCAGCTGGGC CAGTTCCTGC 
     2041 AGCACCGGGA GACGAACCTA CGCTACCTGG CTCTGGAGAG CATGTGCACG CTGGCCAGCT 
     2101 CTGAGTTCTC CCACGAGGCC GTCAAGACCC ACATTGAGAC GGTCATCAAT GCCTTGAAGA 
     2161 CGGAAAGAGA CGTGAGTGTG AGGCAGCGGG CAGTGGACCT GCTCTACGCC ATGTGTGACC 
     2221 GGAGCAACGC CCAGCAGATT GTGGCTGAGA TGCTGAGCTA CCTAGAAACG GCTGACTACT 
     2281 CCATCCGAGA GGAAATTGTG CTGAAGGTGG CCATCCTGGC TGAGAAGTAT GCAGTGGACT 
     2341 ACACTTGGTA TGTGGACACC ATCCTCAACC TCATCCGCAT CGCGGGCGAC TATGTGAGCG 
     2401 AGGAGGTGTG GTACCGCGTC ATCCAGATTG TCATCAATCG CGATGATGTG CAGGGCTATG 
     2461 CTGCCAAGAC GGTATTTGAG GCATTGCAGG CGCCAGCATG TCATGAGAAC TTGGTCAAAG 
     2521 TGGGCGGCTA CATCCTGGGG GAGTTTGGAA ACTTGATAGC TGGGGACCCA CGATCCAGCC 
     2581 CTCTGATCCA GTTCAACCTG CTCCACTCCA AGTTCCACTT GTGCAGCGTC CCCACTCGGG 
     2641 CACTCCTGCT GTCCACCTAC ATCAAGTTCG TGAACCTCTT TCCAGAGGTA AAAGCTACCA 
     2701 TTCAGGACGT GCTGCGCAGT GACAGCCAGC TGAAGAATGC CGACGTGGAG CTGCAGCAGC 
     2761 GGGCTGTGGA GTACCTGCGA CTGAGCACTG TCGCCAGCAC TGACATCCTG GCAACTGTCC 
     2821 TGGAGGAAAT GCCACCCTTC CCTGAGCGTG AGTCCTCCAT CTTGGCCAAG CTGAAGAAGA 
     2881 AGAAGGGCCC AAGCACAGTG ACTGACCTGG AGGAGACCAA GCGGGAGCGA AGCATCGATG 
     2941 TGAATGGGGG CCCTGAACCC GTTCCAGCCA GCACCAGTGC TGCGTCCACA CCTTCTCCGT 
     3001 CAGCAGACCT GCTGGGTCTG GGGGCCGTGC CCCCTGCTCC CACGGGGCCC CCTCCCTCCT 
     3061 CTGGCGGCGG GCTGCTGGTG GACGTGTTCT CAGACTCAGC TTCTGCAGTC GCACCTCTTG 
     3121 CACCCGGCTC TGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAATAATGGT GTATTGTTCG 
     3181 AAAACCAGCT GCTTCAAATT GGACTTAAGT CAGAATTTCG GCAGAATTTA GGCCGAATGT 
     3241 TCATATTTTA TGGTAACAAG ACCTCCACGC AGTTCCTAAA CTTCACTCCA ACACTAATCT 
     3301 GTGCTGACGA CCTTCAAACT AATCTGAACC TGCAGACTAA GCCCGTGGAC CCAACTGTGG 
     3361 ATGGGGGCGC ACAGGTGCAA CAAGTGGTCA ACATTGAGTG TATATCTGAT TTCACAGAGG 
     3421 CACCTGTCCT CAACATCCAG TTCAGGTATG GTGGGACCTT CCAGAATGTG TCTGTTAAGC 
     3481 TTCCCATCAC TCTCAACAAA TTTTTCCAGC CCACAGAAAT GGCTTCTCAG GATTTCTTTC 
     3541 AACGTTGGAA GCAGTTGAGC AATCCACAGC AAGAAGTGCA GAATATCTTC AAAGCAAAGC 
     3601 ATCCGATGGA CACAGAGATC ACTAAGGCAA AGATTATTGG ATTTGGTTCT GCGCTCCTGG 
     3661 AAGAAGTTGA CCCGAATCCT GCAAATTTCG TGGGTGCTGG CATAATACAC ACCAAAACCA 
     3721 CCCAGATTGG CTGCTTGTTG CGCCTGGAGC CAAACCTGCA AGCTCAGATG TATAGACTCA 
     3781 CCTTGCGTAC CAGCAAAGAC ACCGTCTCTC AGAGACTATG TGAATTGCTG TCGGAACAGT 
     3841 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3901 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3961 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4021 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4081 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4141 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4201 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4261 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4321 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4381 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4441 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4501 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4561 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4621 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4681 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4741 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4801 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4861 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4921 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4981 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5041 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5101 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5161 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5221 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5281 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5341 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5401 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5461 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5521 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5581 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5641 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5701 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5761 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5821 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5881 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5941 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6001 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6061 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6121 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6181 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6241 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6301 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6361 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6421 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6481 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6541 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6601 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6661 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6721 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6781 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6841 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6901 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6961 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7021 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7081 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7141 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7201 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7261 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7321 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7381 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7441 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7501 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7561 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7621 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7681 TTTAACGCGA ATTTT
//