LOCUS       MR211197                7650 bp    DNA     circular  SYN 
DEFINITION  Nrp1 (Myc-DDK-tagged) - Mouse neuropilin 1 (Nrp1)
ACCESSION   NM_008737
VERSION     NM_008737.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3807..3898 
                     /label="Myc-DDK"  
     misc_feature    3928..4513 
                     /label="PolyA signal"  
     misc_feature    3985..4004 
                     /label="Restriction Enzyme"  
     misc_feature    4789..5461 
                     /label="ColE1"  
     misc_feature    5891..6685 
                     /label="Kan/Neo"  
     misc_feature    6720..7022 
                     /label="SV40 ori"  
     misc_feature    7108..7585 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3789..3795 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3789 
                     /label="Nrp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGGGGG CTGCCGTTGC TGTGCGCCAC GCTCGCCCTT GCCCTCGCCC 
     1081 TGGCGGGCGC TTTCCGCAGC GACAAATGTG GCGGGACCAT AAAAATCGAA AACCCAGGGT 
     1141 ACCTCACATC TCCCGGTTAC CCTCATTCTT ACCATCCAAG TGAGAAGTGT GAATGGCTAA 
     1201 TCCAAGCTCC GGAACCCTAC CAGAGAATCA TGATCAACTT CAACCCACAT TTCGATTTGG 
     1261 AGGACAGAGA CTGCAAGTAT GACTACGTGG AAGTAATCGA TGGGGAGAAT GAAGGCGGCC 
     1321 GCCTGTGGGG GAAGTTCTGT GGGAAGATTG CACCTTCTCC TGTGGTGTCT TCAGGGCCCT 
     1381 TTCTCTTCAT CAAATTTGTC TCTGACTATG AGACACATGG GGCAGGGTTT TCCATCCGCT 
     1441 ATGAAATCTT CAAGAGAGGG CCCGAATGTT CTCAGAACTA TACAGCACCT ACTGGAGTGA 
     1501 TAAAGTCCCC TGGGTTCCCT GAAAAATACC CCAACAGCTT GGAGTGCACC TACATCATCT 
     1561 TTGCACCAAA GATGTCTGAG ATAATCCTGG AGTTTGAAAG TTTTGACCTG GAGCAAGACT 
     1621 CGAATCCTCC CGGAGGAATG TTCTGTCGCT ATGACCGGCT GGAGATCTGG GATGGATTCC 
     1681 CTGAAGTTGG CCCTCACATT GGGCGTTATT GTGGGCAGAA AACTCCTGGC CGGATCCGCT 
     1741 CCTCTTCAGG CGTTCTATCC ATGGTCTTTT ACACTGACAG CGCAATAGCA AAAGAAGGTT 
     1801 TCTCAGCCAA CTACAGTGTG CTACAGAGCA GCATCTCTGA AGATTTTAAG TGTATGGAGG 
     1861 CTCTGGGCAT GGAATCTGGA GAGATCCATT CTGATCAGAT CACTGCATCT TCACAGTATG 
     1921 GTACCAACTG GTCTGTAGAG CGCTCCCGCC TGAACTACCC TGAAAATGGG TGGACTCCAG 
     1981 GAGAAGACTC CTACAAGGAG TGGATCCAGG TGGACTTGGG CCTCCTGCGA TTCGTTACTG 
     2041 CTGTAGGGAC ACAGGGTGCC ATTTCCAAGG AAACCAAGAA GAAATATTAT GTCAAGACTT 
     2101 ACAGAGTAGA CATCAGCTCC AACGGAGAGG ACTGGATCTC CCTGAAAGAG GGAAATAAAG 
     2161 CCATTATCTT TCAGGGAAAC ACCAACCCCA CAGATGTTGT CTTAGGAGTT TTCTCCAAAC 
     2221 CACTGATAAC TCGATTTGTC CGAATCAAAC CTGTATCCTG GGAAACTGGT ATATCTATGA 
     2281 GATTTGAAGT TTATGGCTGC AAGATAACAG ATTATCCTTG CTCTGGAATG TTGGGCATGG 
     2341 TGTCTGGACT TATTTCAGAC TCCCAGATTA CAGCATCCAA TCAAGCCGAC AGGAATTGGA 
     2401 TGCCAGAAAA CATCCGTCTG GTGACCAGTC GTACCGGCTG GGCACTGCCA CCCTCACCCC 
     2461 ACCCATACAC CAATGAATGG CTCCAAGTGG ACCTGGGAGA TGAGAAGATA GTAAGAGGTG 
     2521 TCATCATTCA GGGTGGGAAG CACCGAGAAA ACAAGGTGTT CATGAGGAAG TTCAAGATCG 
     2581 CCTATAGTAA CAATGGCTCT GACTGGAAAA CTATCATGGA TGACAGCAAG CGCAAGGCTA 
     2641 AGTCGTTCGA AGGCAACAAC AACTATGACA CACCTGAGCT TCGGACGTTT TCACCTCTCT 
     2701 CCACAAGGTT CATCAGGATC TACCCTGAGA GAGCCACACA CAGTGGGCTT GGGCTGAGGA 
     2761 TGGAGCTACT GGGCTGTGAA GTGGAAGCAC CTACAGCTGG ACCAACCACA CCCAATGGGA 
     2821 ACCCAGTGGA TGAGTGTGAC GACGACCAGG CCAACTGCCA CAGTGGCACA GGTGATGACT 
     2881 TCCAGCTCAC AGGAGGCACC ACTGTCCTGG CCACAGAGAA GCCAACCATT ATAGACAGCA 
     2941 CCATCCAATC AGAGTTCCCG ACATACGGTT TTAACTGCGA GTTTGGCTGG GGCTCTCACA 
     3001 AGACATTCTG CCACTGGGAG CATGACAGCC ATGCACAGCT CAGGTGGAGT GTGCTGACCA 
     3061 GCAAGACAGG GCCGATTCAG GACCATACAG GAGATGGCAA CTTCATCTAT TCCCAAGCTG 
     3121 ATGAAAATCA GAAAGGCAAA GTAGCCCGCC TGGTGAGCCC TGTGGTCTAT TCCCAGAGCT 
     3181 CTGCCCACTG TATGACCTTC TGGTATCACA TGTCCGGCTC TCATGTGGGT ACACTGAGGG 
     3241 TCAAACTACG CTACCAGAAG CCAGAGGAAT ATGATCAACT GGTCTGGATG GTGGTTGGGC 
     3301 ACCAAGGAGA CCACTGGAAA GAAGGACGTG TCTTGCTGCA CAAATCTCTG AAACTATATC 
     3361 AGGTTATTTT TGAAGGTGAA ATCGGAAAAG GAAACCTTGG TGGAATTGCT GTGGATGATA 
     3421 TCAGTATTAA CAACCATATT TCTCAGGAAG ACTGTGCAAA ACCAACAGAC CTAGATAAAA 
     3481 AGAACACAGA AATTAAAATT GATGAAACAG GGAGCACTCC AGGATATGAA GGAGAAGGGG 
     3541 AAGGTGACAA GAACATCTCC AGGAAGCCAG GCAATGTGCT TAAGACCCTG GATCCCATCC 
     3601 TGATCACCAT CATAGCCATG AGTGCCCTGG GAGTACTCCT GGGTGCAGTC TGTGGAGTTG 
     3661 TGCTGTACTG TGCCTGTTGG CACAATGGGA TGTCAGAAAG GAACCTATCT GCCCTGGAGA 
     3721 ACTATAACTT TGAACTTGTG GATGGTGTAA AGTTGAAAAA AGATAAACTG AACCCACAGA 
     3781 GTAATTACTC AGAGGCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3841 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3901 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3961 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4021 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4081 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4141 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4201 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4261 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4321 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4381 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4441 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4501 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4561 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4621 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4681 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4741 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4801 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4861 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4921 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4981 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5041 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5101 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5161 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5221 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5281 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5341 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5401 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5461 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5521 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5581 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5641 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5701 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5761 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5821 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5881 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5941 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6001 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6061 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6121 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6181 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6241 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6301 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6361 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6421 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6481 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6541 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6601 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6661 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6721 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6781 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6841 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6901 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6961 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7021 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7081 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7141 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7201 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7261 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7321 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7381 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7441 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7501 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7561 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7621 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//