LOCUS       MR211175                7635 bp    DNA     circular  SYN 
DEFINITION  Gba2 (Myc-DDK-tagged) - Mouse glucosidase beta 2 (Gba2)
ACCESSION   NM_172692
VERSION     NM_172692.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3792..3883 
                     /label="Myc-DDK"  
     misc_feature    3913..4498 
                     /label="PolyA signal"  
     misc_feature    3970..3989 
                     /label="Restriction Enzyme"  
     misc_feature    4774..5446 
                     /label="ColE1"  
     misc_feature    5876..6670 
                     /label="Kan/Neo"  
     misc_feature    6705..7007 
                     /label="SV40 ori"  
     misc_feature    7093..7570 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3780 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="Gba2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTAACCTGC GTCCCGGCCT CAGAGCAGGT CGGCTGTGCC GAAAGAGATT 
     1081 CCCAAGTTTA TTGTGAAGAT ACTGGCGGCA CTGAGGCTGT GCGGGTTACA GACTGCGGGA 
     1141 GCCCCGAGGA TAGTGGACCC CAGGATGAAC CAAGCTACTG CAATTCAGAG GACTCTGGGC 
     1201 AGCTGATGGC CTCCTACGAG GGTAAAGCTA GGGGCTACCA GGTGCCTCCT TTTGGCTGGC 
     1261 GGATCTGCTT GGCTCACGAG TTTGCAGAGA AGAGGAGACC TTTTCAAGCT AACAACATCT 
     1321 CTCTCAGTAA TTTGGTAAAG CACCTCGGTA TGGGCTTGAG GTACTTGAAG TGGTGGTACC 
     1381 GAAAGACCCA CGTGGAAAAG AAGACCCCTT TCATCGACAT GCTCAATTCT CTACCCCTGA 
     1441 GACAGATCTA TGGTTGTCCC CTGGGTGGCA TTGGAGGAGG CACTATCACC CGGGGCTGGA 
     1501 GAGGCCAGTT CTGTCGTTGG CAGCTCAACC CTGGAATGTA CCAGCACCAG ACAGTCATTG 
     1561 CAGACCAATT TATAGTATGC TTGCGTCGAG ATGGGCGGAC TGTGTACCAG CAAGTTCTGT 
     1621 CCTTGGAGCT TCCAAATGTC CTGCGCAGCT GGAACTGGGG CCTGTGTGGT TACTTTGCTT 
     1681 TCTACCACGC CCTCTATCCC CGAGCCTGGA CGGTCTATCA GCTTCCTGGC CAGAATGTCA 
     1741 CCCTCACCTG TCGCCAGGTC ACACCTATCT TGCCTCATGA CTACCAGGAC AGCAGTCTCC 
     1801 CTGTAGGAGT CTTTGTGTGG GATGTAGAAA ACGAAGGAGA TGAGACTCTG GACGTTTCCA 
     1861 TCACGTTCTC CATGCGGAAT GGACTAGGAG GCGAAGATGA TGCGGCCGGG AGTTTGTGGA 
     1921 ATGAGCCCTT CCGCCTGGAA CAGGGCGGGA CGACTGTTCA GGGGCTCCTC TTGCATCATC 
     1981 CAACCCCTCC GAACCCCTAC ACCATGGCTG TGGCTGCACG ATGCACGGCA GATACCACGG 
     2041 TAACCCACAC CACAGCCTTT GACCCCAATG GCACTGGACA GCAGGTGTGG CAGGACCTAC 
     2101 TTCAGGATGG ACAGCTGGAC TCCCCTGCTG GCCAAAGCAC CCCAACACAG AAAGGAGAGG 
     2161 GTATCGCTGG GGCTGTATGC GTCTCTAGCA AGCTGTTACC TCGAAGCCGG TGCTGCTTGG 
     2221 AGTTCTCACT GGCTTGGGAT ATGCCTAAGA TCATGTTTGG AGCTAAAAGC CAAGTCCACT 
     2281 ACAGGCGGTA TACACGGTTC TTTGGTTCAG ATGGTGATGT GGCGCCTGCC CTGAGCCACT 
     2341 ATGCACTGTG CCACTATGCA GACTGGGAGG ACAGAATCTC AGCCTGGCAG AACCCAGTAC 
     2401 TGGATGACAG AACCTTGCCT GCCTGGTACA AATCTGCACT GTTCAATGAA CTGTACTTCC 
     2461 TGGCCGATGG AGGCACAGTG TGGCTGGAAG TTCCCGCAGA CTCCCTACCA GAGGGGCTGG 
     2521 GAGGGAGTAT GCGTCAGCTT CGCTCTACTC TGCAGGACTA TGGGCGATTT GGCTATCTTG 
     2581 AAGGCCAGGA GTACCGCATG TACAACACAT ACGATGTCCA CTTTTATGCG TCCTTTGCCC 
     2641 TCGTCATGCT GTGGCCCAAA CTTGAGCTCA GTCTTCAGTA TGATATGGCT CTGGCAACTT 
     2701 TGAAGGAGGA CCTGACCCGG CGACGGTACC TGATGAGTGG AGTGGTGGCA CCTGTGAAAA 
     2761 GGAGGAACGT CATCCCTCAT GACATTGGGG ATCCGGATGA TGAGCCATGG CTCCGGGTCA 
     2821 ACGCATATTT GATTCATGAT ACTGCTGACT GGAAGGACCT GAACCTGAAG TTTGTATTGC 
     2881 AAATTTATCG GGACTATTAC CTGACGGGTG ATCAAGGCTT CCTGGAGGAC ATGTGGCCTG 
     2941 TGTGTCTGGC TGTGATGGAG TCCGAAATGA AGTTTGACAA GGACCAAGAT GGACTCATTG 
     3001 AGAATGGAGG CTACGCAGAC CAGACCTATG ATGCATGGGT CACCACAGGC CCCAGTGCTT 
     3061 ACTGCGGAGG GCTGTGGCTG GCGGCAGTGG CTGTAATGGT TCAGATGGCT GTTCTGTGTG 
     3121 GGGCCCAAGA TGTCCAGGAG AGGTTTGCTT CCATTCTCTG CCGAGGCCGA GAAGCTTATG 
     3181 AGAGACTGCT GTGGAACGGA CGCTATTACA ACTACGACAG CAGCTCCCAT CCTCAGTCTC 
     3241 GGAGCATCAT GTCTGACCAG TGTGCTGGGC AGTGGTTCCT GAGGGCCTGC GGCCTGGGAG 
     3301 AAGGAGACAC TGAAGTATTT CCTACCCTGC ATGTGGTCCG TGCTCTCCAA ACCATCTTTG 
     3361 AACTCAACGT CCAAGCCTTT GCAGGAGGAG CCATGGGGGC CGTGAACGGG ATGCACCCTC 
     3421 ATGGTGTCCC TGATAGATCC AGTGTGCAGT CTGATGAAGT CTGGGTGGGT GTGGTCTATG 
     3481 GGCTGGCAGC CACCATGATC CAAGAGGGCC TGACTTGGGA AGGTTTCCGG ACAGCTGAAG 
     3541 GCTGTTACCG CACTGTATGG GAACGCCTGG GCCTGGCTTT CCAGACCCCA GAGGCATACT 
     3601 GCCAGCAACA AGTGTTCCGC TCCCTGGCCT ACATGCGGCC ACTGAGCATC TGGGCCATGC 
     3661 AGCTGGCCCT GCAACAGCAG CAGCATAAAA AGAGCCGCAG GCCATCAGTC ACACAAGGCA 
     3721 CGGGACTAAG CACACAGCCT GAATGTGGAC CAAAGAGATC GCTGGCAAAC CTCAATTCAG 
     3781 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3841 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3901 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3961 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4021 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4081 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4141 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4201 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4261 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4321 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4381 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4441 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4501 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4561 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4621 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4681 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4741 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4801 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4861 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4921 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4981 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5041 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5101 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5161 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5221 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5281 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5341 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5401 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5461 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5521 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5581 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5641 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5701 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5761 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5821 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5881 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5941 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6001 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6061 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6121 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6181 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6241 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6301 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6361 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6421 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6481 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6541 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6601 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6661 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6721 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6781 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6841 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6901 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6961 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7021 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7081 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7141 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7201 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7261 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7321 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7381 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7441 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7501 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7561 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7621 TTTAACGCGA ATTTT
//