LOCUS       MR211170                7632 bp    DNA     circular  SYN 
DEFINITION  Hk2 (Myc-DDK-tagged) - Mouse hexokinase 2 (Hk2)
ACCESSION   NM_013820
VERSION     NM_013820.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3789..3880 
                     /label="Myc-DDK"  
     misc_feature    3910..4495 
                     /label="PolyA signal"  
     misc_feature    3967..3986 
                     /label="Restriction Enzyme"  
     misc_feature    4771..5443 
                     /label="ColE1"  
     misc_feature    5873..6667 
                     /label="Kan/Neo"  
     misc_feature    6702..7004 
                     /label="SV40 ori"  
     misc_feature    7090..7567 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3771..3777 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3771 
                     /label="Hk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCGCCTCG CATATGATCG CCTGCTTATT CACGGAGCTC AACCAAAACC 
     1081 AAGTGCAGAA GGTTGACCAG TATCTCTACC ACATGCGTCT CTCAGATGAG ACCCTTCTGG 
     1141 AGATTTCTAG GCGGTTCCGG AAGGAGATGG AGAAGGGGCT AGGAGCTACC ACACACCCTA 
     1201 CAGCAGCTGT GAAGATGCTG CCCACCTTTG TGAGGTCAAC TCCGGATGGG ACAGAACATG 
     1261 GCGAGTTCCT GGCTCTGGAC CTTGGAGGAA CCAACTTCCG TGTGCTCCGA GTAAGGGTGA 
     1321 CAGACAATGG TCTCCAGAGG GTGGAGATGG AGAACCAGAT CTACGCCATT CCCGAGGACA 
     1381 TCATGCGGGG CAGTGGAACC CAGCTGTTTG ACCACATTGC CGAATGCCTG GCCAACTTCA 
     1441 TGGACAAGCT ACAGATCAAA GAGAAGAAGC TCCCGCTGGG TTTCACCTTC TCGTTCCCCT 
     1501 GCCACCAGAC GAAACTGGAT GAGAGTTTCT TGGTCTCATG GACTAAGGGG TTCAAGTCCA 
     1561 GTGGCGTGGA AGGCAGAGAT GTGGTGGATC TGATCCGGAA GGCCATCCAG CGCAGAGGGG 
     1621 ACTTTGACAT TGACATTGTG GCTGTGGTGA ATGACACGGT TGGGACCATG ATGACTTGTG 
     1681 GTTATGATGA TCAGAACTGC GAGATTGGTC TCATTGTGGG TACTGGCAGT AATGCCTGCT 
     1741 ACATGGAGGA GATGCGTCAC ATTGACATGG TGGAGGGCGA CGAGGGACGG ATGTGCATCA 
     1801 ACATGGAGTG GGGGGCCTTT GGGGACGACG GTACACTCAA TGACATCCGA ACTGAGTTTG 
     1861 ACAGAGAGAT CGACATGGGC TCACTGAACC CTGGGAAGCA GCTGTTTGAG AAGATGATCA 
     1921 GCGGGATGTA CATGGGGGAG CTGGTCAGAC TCATCCTGGT GAAGATGGCC AAGGCCGAGC 
     1981 TGCTGTTCCA AGGGAAACTC AGCCCAGAGC TCCTTACCAC TGGCTCCTTC GAGACCAAAG 
     2041 ATGTCTCGGA TATTGAAGAC GATAAGGACG GAATTCAGAA GGCCTACCAG ATCCTGGTGC 
     2101 GCCTGGGTCT GAGCCCGCTG CAGGAGGACT GTGTGGCCAC GCACCGAATC TGCCAGATTG 
     2161 TGTCCACGCG CTCAGCCAGC CTGTGTGCAG CCACCCTGGC CGCGGTGCTG TGGCGGATCA 
     2221 AAGAGAACAA GGGCGAGGAG CGACTCCGCT CCACCATCGG CGTGGATGGC TCTGTCTACA 
     2281 AGAAACATCC CCATTTTGCC AAGCGTCTCC ATAAGGCAGT GAGGAGGCTG GTGCCCGATT 
     2341 GTGATGTCCG CTTCCTCCGC TCTGAGGATG GCAGCGGCAA GGGGGCGGCT ATGGTGACGG 
     2401 CGGTGGCTTA CCGTCTGGCT GACCAACACC GGGCCCGCCA GAAGACCCTG GAGTCTCTGA 
     2461 AGCTGAGCCA TGAACAGCTG CTGGAGGTTA AGAGAAGGAT GAAGGTGGAA ATGGAGCAGG 
     2521 GTCTGAGCAA GGAGACGCAT GAGGCCGCCC CTGTGAAGAT GTTGCCCACT TATGTGTGTG 
     2581 CCACTCCAGA CGGTACAGAG AAAGGAGACT TCCTGGCCTT GGATCTTGGA GGAACAAACT 
     2641 TCCGGGTCCT GCTGGTGCGC GTGCGTAATG GCAAGCGAAG GGGCGTAGAG ATGCATAACA 
     2701 AGATCTACTC CATCCCACAG GAAGTCATGC ATGGCACTGG AGAAGAGCTT TTCGACCACA 
     2761 TTGTCCAGTG CATCGCGGAC TTCCTTGAGT ACATGGGCAT GAAGGGCGTG TCCCTACCTT 
     2821 TGGGTTTCAC CTTCTCCTTC CCTTGCCAGC AGAACAGCCT GGACCAGAGC ATCCTCCTCA 
     2881 AGTGGACAAA GGGATTCAAG GCATCCGGCT GCGAGGGTGA AGATGTGGTC ACCTTGCTGA 
     2941 AGGAAGCCAT TCGCCGGCGA GAGGAGTTTG ACCTGGATGT GGTGGCCGTG GTAAATGACA 
     3001 CAGTTGGGAC TATGATGACT TGTGGCTATG AAGACCCTCA CTGTGAAGTT GGCCTCATTG 
     3061 TTGGCACTGG AAGCAATGCC TGCTACATGG AGGAGATGCG TAATGTGGAA CTGGTGGACG 
     3121 GAGAGGAGGG GCGGATGTGT GTCAACATGG AGTGGGGAGC GTTCGGGGAC AACGGCTGCC 
     3181 TGGATGACCT GCGCACAGTG TTTGACGTCG CTGTGGACGA GCTCTCTCTC AACCCTGGCA 
     3241 AACAGAGGTT TGAGAAGATG ATCAGCGGCA TGTACTTGGG CGAGATTGTG CGCAACATCC 
     3301 TGATCGATTT CACAAAGCGG GGGCTGCTCT TCCGTGGCCG CATCTCAGAG CGCCTCAAGA 
     3361 CAAGGGGAAT CTTCGAAACT AAGTTCCTGT CTCAGATCGA GAGTGACTGC CTGGCCCTGC 
     3421 TACAGGTCCG AGCCATCTTG CGCCACCTAG GGCTGGAGAG CACGTGTGAC GACAGCATCA 
     3481 TTGTGAAGGA GGTGTGCACT GTGGTCGCCC GGCGCGCGGC CCAGCTCTGT GGCGCAGGCA 
     3541 TGGCCGCCGT GGTGGACAAG ATAAGAGAGA ACCGTGGACT GGACAACCTC AAAGTGACGG 
     3601 TGGGCGTGGA CGGGACACTG TACAAGCTTC ATCCTCACTT TGCCAAAGTC ATGCATGAGA 
     3661 CGGTGAGAGA TCTGGCTCCG AAATGTGACG TGTCCTTCCT GGAATCCGAG GACGGCAGTG 
     3721 GGAAGGGAGC GGCTCTCATC ACTGCTGTGG CCTGTCGCAT CCGGGAGGCC GGGCAGAGAA 
     3781 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3841 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3901 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3961 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4021 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4081 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4141 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4201 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4261 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4321 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4381 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4441 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4501 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4561 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4621 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4681 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4741 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4801 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4861 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4921 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4981 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5041 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5101 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5161 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5221 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5281 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5341 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5401 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5461 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5521 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5581 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5641 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5701 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5761 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5821 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5881 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5941 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6001 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6061 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6121 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6181 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6241 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6301 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6361 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6421 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6481 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6541 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6601 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6661 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6721 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6781 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6841 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6901 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6961 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7021 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7081 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7141 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7201 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7261 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7321 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7381 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7441 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7501 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7561 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7621 AACGCGAATT TT
//