LOCUS       MR211169                7629 bp    DNA     circular  SYN 
DEFINITION  Lig1 (Myc-DDK-tagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 1
ACCESSION   NM_001083188
VERSION     NM_001083188.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3786..3877 
                     /label="Myc-DDK"  
     misc_feature    3907..4492 
                     /label="PolyA signal"  
     misc_feature    3964..3983 
                     /label="Restriction Enzyme"  
     misc_feature    4768..5440 
                     /label="ColE1"  
     misc_feature    5870..6664 
                     /label="Kan/Neo"  
     misc_feature    6699..7001 
                     /label="SV40 ori"  
     misc_feature    7087..7564 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3768..3774 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3768 
                     /label="Lig1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAGAAGT ATTATGTCAT TTTTTCAACC CACAAAAGAA GGTAAAGCGA 
     1081 AGAAGCCAGA GAAGGAGACA CCCAGCAGCA TCAGAGAGAA GGAACCCCCT CCAAAGGTGG 
     1141 CGCTGAAGGA GAGGAATCAA GTGGTGCCCG AGAGTGATTC TCCAGTGAAG AGGACAGGAA 
     1201 GGAAGGTAGC CCAGGTTCTG AGCTGTGAAG GGGAGGACGA AGATGAAGCC CCTGGCACCC 
     1261 CCAAAGTCCA GAAGCCTGTG TCAGACTCTG AACAGAGCTC TCCTCCCAGC CCTGACACAT 
     1321 GTCCTGAGAA CAGTCCTGTC TTCAACTGCA GCTCCCCCAT GGACATCTCC CCATCAGGAT 
     1381 TCCCAAAGCG TCGAACTGCG CGGAAGCAGC TCCCCAAACG GACAATTCAA GACACTCTTG 
     1441 AGGAGCAGAA TGAGGACAAA ACCAAAACAG CTAAGAAAAG GAAGAAAGAA GAAGAGACCC 
     1501 CAAAAGAAAG CCTCGCAGAG GCTGAAGACG TAAAACAGAA GGAAGAAAAG GAGGGGGACC 
     1561 AGCTCATAGT CCCCTCTGAG CCCACAAAGT CCCCTGAGTC AGTAACCCTG ACAAAGACAG 
     1621 AGAACATTCC AGTGTGTAAG GCAGGAGTGA AACTGAAGCC TCAGGAAGAG GAACAGAGCA 
     1681 AACCTCCTGC TAGAGGTGCC AAGACACTGA GCAGTTTCTT CACACCCCGA AAACCAGCAG 
     1741 TGAAAACTGA AGTCAAACAA GAAGAGTCAG GCACTCTAAG GAAGGAAGAG ACCAAGGGAA 
     1801 CCCTGGATCC AGCAAATTAT AATCCTTCCA AGAATAACTA TCATCCCATT GAAGATGCCT 
     1861 GCTGGAAACA TGGCCAGAAG GTCCCTTTTC TCGCCGTGGC CCGGACATTT GAGAAGATTG 
     1921 AGGAGGTTTC TGCTAGGCTC AAGATGGTGG AGACATTGAG CAACCTGCTG CGCTCCGTGG 
     1981 TGGCCCTGTC CCCTCCAGAC TTGCTCCCTG TTCTCTATCT CAGCCTCAAC CGCCTCGGGC 
     2041 CACCTCAGCA GGGACTAGAG CTGGGTGTTG GTGATGGTGT CCTCCTCAAG GCCGTTGCCC 
     2101 AGGCCACAGG TCGTCAGCTG GAGTCCATCC GGGCTGAGGT AGCTGAGAAG GGGGATGTGG 
     2161 GGCTGGTGGC CGAGAACAGC CGCAGCACTC AGAGACTCAT GCTGCCTCCA CCTCCACTTA 
     2221 CTATTTCTGG AGTCTTCACC AAATTCTGTG ACATTGCCCG GCTCACTGGC AGTGCTTCCA 
     2281 TGGCCAAGAA GATGGACATT ATCAAGGGCC TTTTTGTTGC CTGCCGCCAC TCAGAAGCCA 
     2341 GGTACATTGC CAGGTCCCTA AGCGGGCGGC TGCGCCTTGG CCTAGCTGAG CAGTCGGTAC 
     2401 TGGCTGCTCT TGCCCAGGCT GTGAGCCTCA CACCCCCTGG CCAAGAATTT CCCACGGCGG 
     2461 TGGTGGATGC TGGGAAGGGC AAGACAGCAG AGGCCAGAAA GATGTGGTTG GAAGAACAAG 
     2521 GCATGATCTT GAAGCAGACC TTCTGTGAGG TTCCTGACCT GGATCGAATC ATCCCTGTGC 
     2581 TGCTGGAACA TGGCCTGGAA CGTCTCCCAG AGCACTGCAA GCTGAGCCCA GGGGTCCCTC 
     2641 TTAAACCAAT GCTGGCTCAT CCCACTCGGG GTGTCAGTGA GGTCCTAAAA CGCTTTGAGG 
     2701 AGGTCGACTT CACCTGTGAA TATAAATACG ATGGGCAGCG AGCACAGATA CATGTTCTGG 
     2761 AAGGTGGAGA AGTGAAGATC TTCAGCAGGA ACCAGGAAGA CAACACAGGA AAGTATCCTG 
     2821 ACATTATCAG CCGCATCCCC AAGATTAAAC ACCCCTCAGT CACATCTTTC ATTCTGGACA 
     2881 CTGAGGCTGT GGCCTGGGAC CGGGAAAAGA AGCAGATCCA ACCATTCCAA GTACTCACCA 
     2941 CGCGCAAGCG CAAGGAGGTT GATGCATCGG AGATACAGGT GCAAGTGTGT CTGTATGCTT 
     3001 TTGACCTCAT CTACCTCAAC GGAGAGTCCC TGGTTCGCCA GCCCCTGTCT CGACGCCGGC 
     3061 AGCTGCTCCG TGAGAACTTC GTGGAGACAG AGGGTGAGTT TGTCTTCACC ACCTCTTTGG 
     3121 ACACCAAGGA CACCGAGCAG ATTGCTGAGT TCTTGGAGCA GTCAGTGAAG GACTCCTGTG 
     3181 AGGGCCTGAT GGTGAAGACC TTGGATGTTG ATGCCACTTA TGAGATTGCC AAGAGGTCAC 
     3241 ACAACTGGCT CAAGCTGAAG AAGGACTACC TTGACGGTGT GGGTGACACT CTGGACCTTG 
     3301 TGGTGATTGG TGCCTACCTG GGCCGGGGGA AGCGGGCTGG CCGGTATGGA GGCTTCCTGT 
     3361 TGGCTGCCTA CGATGAGGAG AGTGAAGAGC TTCAGGCTAT ATGCAAGCTG GGAACTGGAT 
     3421 TCAGCGATGA GGAGCTGGAG GAACACCACC AGAGCCTGCA GGCCCTGGTA TTGCCTACCC 
     3481 CACGCCCCTA TGTGAGGATT GATGGGGCAG TGGCCCCAGA CCATTGGCTG GACCCAAGCA 
     3541 TTGTGTGGGA GGTGAAGTGT GCTGATCTTT CCCTGTCCCC CATCTACCCT GCTGCACGGG 
     3601 GCCTGGTGGA CAAAGAAAAA GGGATCTCTC TTCGTTTTCC TCGGTTCATT CGTGTCCGTA 
     3661 AAGACAAGCA GCCAGAGCAG GCCACTACCA GTAACCAGGT TGCCTCTTTG TACCGGAAGC 
     3721 AGAGTCAGAT TCAGAACCAA CAAAGCTCAG ACTTGGACTC CGACGTTGAA GACTATACGC 
     3781 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3841 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3901 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3961 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4021 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4081 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4141 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4201 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4261 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4321 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4381 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4441 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4501 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4561 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4621 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4681 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4741 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4801 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4861 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4921 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4981 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5041 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5101 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5161 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5221 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5281 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5341 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5401 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5461 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5521 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5581 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5641 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5701 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5761 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5821 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5881 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5941 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6001 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6061 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6121 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6181 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6241 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6301 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6361 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6421 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6481 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6541 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6601 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6661 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6721 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6781 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6841 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6901 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6961 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7021 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7081 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7141 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7201 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7261 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7321 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7381 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7441 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7501 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7561 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7621 GCGAATTTT
//