LOCUS       MR211123                7593 bp    DNA     circular  SYN 
DEFINITION  Mcm2 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 2 mitotin (S. cerevisiae) (Mcm2)
ACCESSION   NM_008564
VERSION     NM_008564.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3750..3841 
                     /label="Myc-DDK"  
     misc_feature    3871..4456 
                     /label="PolyA signal"  
     misc_feature    3928..3947 
                     /label="Restriction Enzyme"  
     misc_feature    4732..5404 
                     /label="ColE1"  
     misc_feature    5834..6628 
                     /label="Kan/Neo"  
     misc_feature    6663..6965 
                     /label="SV40 ori"  
     misc_feature    7051..7528 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3732..3738 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3732 
                     /label="Mcm2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGTCT TCTGAGTCTC TCTCAGCATC TAGCCCTGCC CGTCAGCGGC 
     1081 GCCGGATCAG TGATCCCCTC ACCTCCAGCC CAGGCCGCAG CTCCAGACGT GCTGACGCCC 
     1141 TGACCTCCAG CCCTGGCAGA GACCTCCCCC CATTTGAAGA TGAGTCTGAG GGGCTGCTGG 
     1201 GCACAGAGGG GCCCATGGAG GAAGAAGAGG ATGGAGAGGA ACTCATTGGT GATGGCATGG 
     1261 AGAGAGACTA CCGTCCCATT CCGGAGCTCG ATGTCTACGA GGCCGAGGGA TTGGCCCTGG 
     1321 ATGATGAAGA TGTGGAGGAG CTGACAGCCA GTCAGAGAGA GGCAGCTGAG CGGACCATGA 
     1381 GGCAGCGGGA CCGTGAGGCT GGCAGAGGCC TGGGACGCAT GCGCCGGGGG CTGCTCTATG 
     1441 ACAGCAGCGA GGAAGATGAG GAGCGGCCTG CCCGTAAGCG CCGCCACGTA GAACGCGCCA 
     1501 CAGAGGATGG CGAGGAGGAT GAAGAGATGA TCGAGAGTAT TGAGAATCTG GAGGACCTCA 
     1561 AGGGCCACTC GGTGCGCGAG TGGGTGAGCA TGGCAGGGCC CAGGCTGGAG ATCCACCACC 
     1621 GCTTCAAGAA CTTCCTGCGC ACCCACGTGG ACAGCCATGG CCACAACGTC TTCAAGGAGC 
     1681 GCATCAGTGA TATGTGCAAA GAGAACCGTG AGAGTTTGGT GGTAAATTAT GAAGACCTGG 
     1741 CAGCCCGGGA GCACGTGTTG GCATACTTCC TGCCGGAAGC ACCGGCTGAG TTGCTGCAGA 
     1801 TCTTTGACGA GGCTGCCCTG GAGGTCGTGT TGGCCATGTA CCCTAAATAT GACCGTATCA 
     1861 CCAACCACAT CCATGTGCGC ATCTCCCACC TGCCTCTGGT GGAGGAGCTG CGTTCACTGA 
     1921 GGCAGTTGCA CCTGAACCAG CTGATCCGTA CCAGTGGCGT GGTGACCAGC TGCACCGGAG 
     1981 TCCTGCCCCA GCTCAGCATG GTCAAGTACA ACTGTAGCAA GTGCAACTTT GTACTGGGGC 
     2041 CTTTCTGCCA GTCTCAGAAT CAGGAGGTGA AGCCTGGCTC CTGCCCTGAG TGCCAGTCTG 
     2101 CTGGGCCCTT TGAGATCAAC ATGGAGGAGA CCATCTATCA GAACTACCAA CGTATCCGCA 
     2161 TCCAGGAGAG TCCCGGCAAG GTGGCGGCTG GCCGACTGCC CCGTTCCAAG GATGCCATTC 
     2221 TCCTCGCTGA TCTGGTGGAC AGCTGCAAGC CAGGGGACGA GATAGAGCTG ACCGGCATTT 
     2281 ACCATAATAA CTATGACGGC TCGCTTAACA CCGCCAACGG CTTTCCAGTC TTTGCCACTA 
     2341 TTATCTTGGC CAACCATGTT GCCAAGAAGG ACAACAAAGT AGCTGTGGGG GAGCTCACCG 
     2401 ATGAGGACGT GAAGATGATC ACCGGTCTCT CCAAGGATCA GCAAATTGGA GAGAAGATCT 
     2461 TTGCCAGCAT TGCACCCTCC ATCTATGGGC ATGAAGACAT CAAGAGAGGC CTGGCTCTGG 
     2521 CCCTGTTTGG AGGGGAGCCC AAGAACCCAG GTGGAAAGCA CAAGGTTCGA GGTGACATTA 
     2581 ATGTGCTCTT GTGTGGGGAC CCTGGCACAG CAAAGTCCCA ATTCCTCAAA TACATCGAGA 
     2641 AAGTGTCTAG CCGTGCCATC TTCACCACTG GCCAGGGTGC GTCAGCAGTG GGTCTCACCG 
     2701 CGTACGTTCA GCGGCATCCC GTCAGCAGAG AGTGGACCTT AGAGGCGGGA GCCCTGGTTC 
     2761 TGGCTGACCG GGGGGTGTGT CTCATTGACG AGTTTGACAA GATGAATGAC CAGGACAGGA 
     2821 CCAGCATCCA CGAGGCCATG GAACAGCAAA GCATCTCCAT CTCCAAGGCT GGCATCGTTA 
     2881 CCTCGCTGCA AGCCCGCTGC ACTGTCATAG CTGCTGCCAA CCCCATAGGA GGCCGCTACG 
     2941 ACCCTTCACT GACCTTCTCA GAGAATGTAG ACCTCACAGA GCCCATCATT TCCCGCTTTG 
     3001 ATGTCCTGTG TGTGGTGAGG GACACTGTTG ATCCAGTTCA GGATGAGATG CTGGCCCGCT 
     3061 TTGTGGTTGG CAGCCACGTC AGACACCACC CCAGTAACAA GAAGGATGAA GGGTTGACTA 
     3121 ATGGTGGCAC CTTGGAGCCA GCCATGCCCA ACACATATGG CGTGGAGCCC CTGCCTCAGG 
     3181 AGGTGCTGAA GAAGTATATC ATCTATGCCA AGGAGAGGGT CCGCCCGAAG CTCAACCAGA 
     3241 TGGACCAGGA TAAAGTGGCC AGGATGTACA GTGACCTGAG GAAGGAGTCC ATGGCAACGG 
     3301 GCAGCATTCC CATCACGGTG CGCCACATCG AGTCCATGAT CCGCATGGCC GAGGCCCATG 
     3361 CCCGCATGCA CCTGCGGGAC TACGTGATGG AAGACGATGT CAACATGGCC ATCCGAGTGA 
     3421 TGATGGAGAG CTTCATTGAC ACCCAGAAGT TCAGCGTCAT GCGGAGTATG CGCAAGACTT 
     3481 TTGCCCGGTA TCTCTCCTTC CGGCGAGATA ACAATGATCT GCTGCTCTTC ATACTGAAGC 
     3541 AGTTGGTGGC TGAGCAGGTG ACATATCAAC GCAACCGCTT TGGGGCCCAG CAGGACACCA 
     3601 TTGAAATACC TGAGAAGGAT CTGATGGACA AGGCCAGGCA GATCAATATT CACAACCTCT 
     3661 CTGCCTTCTA CGACAGCGAC CTCTTCAAAT TCAACAAGTT CAGCCGTGAC CTGAAACGCA 
     3721 AACTGATCCT ACAGCAGTTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3781 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3841 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3901 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3961 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4021 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4081 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4141 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4201 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4261 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4321 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4381 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4441 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4501 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4561 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4621 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4681 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4741 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4801 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4861 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4921 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4981 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5041 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5101 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5161 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5221 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5281 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5341 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5401 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5461 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5521 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5581 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5641 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5701 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5761 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5821 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5881 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5941 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6001 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6061 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6121 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6181 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6241 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6301 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6361 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6421 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6481 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6541 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6601 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6661 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6721 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6781 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6841 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6961 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7021 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7141 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7201 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7261 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7321 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7381 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7441 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7501 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7561 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//