LOCUS       MR211114L1                9081 bp    DNA     circular  SYN 
DEFINITION  Gria4 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, AMPA4 (alpha 4) (Gria4), transcript variant 1
ACCESSION   NM_019691
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7372..7552 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8280..8939 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7711..8279 
                     /label="ColE1"  
     misc_feature    6247..6267 
                     /label="LR50 primer"  
     misc_feature    6044..6115 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6256..6847 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6026..6032 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6026 
                     /label="Gria4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGGATTATT TGCAGGCAGA TTGTCTTGTT 
     3361 GTTTTCTGGA TTTTGGGGAC TCGCCATGGG AGCCTTTCCG AGCAGCGTTC AAATAGGTGG 
     3421 TCTCTTTATC CGAAACACAG ACCAGGAATA CACTGCTTTT CGGCTAGCTA TCTTTCTTCA 
     3481 TAACACCAGC CCCAATGCAT CTGAAGCCCC TTTCAATTTG GTACCTCATG TGGACAACAT 
     3541 TGAGACTGCC AACAGTTTTG CTGTGACAAA TGCATTCTGT TCCCAGTATT CTAGAGGGGT 
     3601 GTTTGCCATT TTTGGACTCT ATGACAAGAG GTCAGTGCAT ACCTTGACCT CCTTCTGCAG 
     3661 TGCTCTGCAC ATCTCTCTCA TCACACCAAG CTTCCCCACT GAAGGAGAGA GCCAGTTCGT 
     3721 GCTTCAGCTA AGACCTTCAT TGAGAGGTGC ACTCCTGAGC CTCCTGGATC ACTATGAATG 
     3781 GAATTGTTTT GTCTTCCTGT ATGATACAGA CAGGGGGTAT TCAATACTTC AAGCTATAAT 
     3841 GGAAAAAGCA GGACAGAATG GATGGCATGT CAGTGCGATA TGTGTGGAAA ATTTTAACGA 
     3901 TGTCAGCTAC AGGCAACTAC TAGAAGAGCT TGACAGAAGA CAAGAGAAGA AATTTGTAAT 
     3961 AGATTGTGAG ATAGAAAGGC TTCAAAACAT ATTAGAACAA ATTGTGAGTG TTGGGAAGCA 
     4021 CGTCAAAGGC TACCATTATA TCATCGCAAA TTTGGGTTTC AAAGATATTT CTCTTGAGAG 
     4081 ATTTATACAT GGAGGAGCAA ATGTCACTGG ATTCCAGTTA GTAGATTTTA ATACGCCCAT 
     4141 GGTGACGAAA CTAATGGATC GCTGGAAGAA ACTAGATCAA CGAGAATATC CAGGATCTGA 
     4201 AACACCTCCA AAGTACACTT CTGCTCTCAC TTACGATGGT GTCTTGGTAA TGGCTGAAAC 
     4261 TTTCCGAAGT CTCAGAAGAC AGAAAATTGA TATTTCAAGG AGAGGAAATG CCGGGGATTG 
     4321 TCTGGCAAAC CCTGCTGCTC CCTGGGGCCA GGGAATTGAC ATGGAGAGAA CACTGAAGCA 
     4381 GGTTCGAATT CAAGGACTGA CTGGGAATGT TCAATTTGAC CACTATGGAC GTAGAGTTAA 
     4441 TTACACAATG GATGTGTTTG AATTAAAAAG CACAGGACCT CGAAAGGTTG GCTATTGGAA 
     4501 CGATATGGAT AAATTAGTCT TGATTCAAGA TGCGCCTACT CTTGGCAATG ACACAGCAGC 
     4561 TATCGAGAAC AGAACAGTGG TTGTAACCAC AATTATGGAA TCCCCCTACG TTATGTACAA 
     4621 GAAAAATCAT GAAATGTTTG AAGGAAATGA CAAGTATGAA GGCTACTGTG TAGACTTGGC 
     4681 ATCTGAAATT GCGAAACATA TCGGTATCAA ATATAAAATT GCCATTGTCC CTGATGGAAA 
     4741 ATATGGAGCA AGGGATGCGG ACACCAAAAT TTGGAATGGG ATGGTAGGAG AGCTTGTGTA 
     4801 TGGGAAAGCA GAGATTGCCA TTGCACCTCT GACAATCACG TTGGTGCGAG AGGAGGTCAT 
     4861 CGACTTTTCT AAGCCTTTTA TGAGTTTAGG CATCTCTATC ATGATCAAAA AACCTCAGAA 
     4921 ATCTAAACCA GGAGTGTTTT CCTTCTTGGA CCCTCTGGCC TATGAGATCT GGATGTGCAT 
     4981 AGTGTTTGCA TACATTGGTG TCAGCGTGGT CTTGTTCCTA GTCAGTAGGT TTAGCCCATA 
     5041 TGAGTGGCAC ACAGAAGAGC CTGAGGATGG AAAAGAAGGA CCCAGTGACC AACCTCCCAA 
     5101 TGAGTTTGGC ATCTTTAACA GCCTCTGGTT TTCCCTGGGT GCCTTTATGC AACAAGGATG 
     5161 TGACATTTCA CCCAGATCCC TGTCCGGTCG GATTGTTGGA GGCGTATGGT GGTTCTTCAC 
     5221 TCTCATCATT ATCTCATCCT ACACTGCTAA TCTGGCTGCA TTCCTGACAG TGGAGAGAAT 
     5281 GGTCTCCCCC ATAGAAAGTG CAGAAGACCT GGCCAAACAA ACAGAAATTG CCTATGGAAC 
     5341 ACTTGATTCG GGATCAACAA AAGAATTCTT CAGAAGATCA AAAATAGCAG TATATGAAAA 
     5401 GATGTGGACC TACATGCGAT CGGCAGAGCC ATCTGTGTTC ACTAGAACTA CAGCTGAGGG 
     5461 CGTGGCCCGT GTCCGCAAGT CCAAGGGCAA ATTTGCCTTC CTCCTGGAGT CCACGATGAA 
     5521 TGAATACATT GAGCAGCGAA AGCCCTGTGA CACGATGAAA GTGGGAGGAA ACCTGGATTC 
     5581 CAAAGGCTAT GGTGTAGCGA CGCCCAAGGG TTCCTCATTA GGAACTCCTG TAAACCTTGC 
     5641 CGTTTTGAAA CTCAGTGAGG CAGGCGTCTT AGACAAGCTG AAAAACAAAT GGTGGTACGA 
     5701 TAAAGGTGAA TGTGGACCCA AGGACTCGGG AAGCAAGGAC AAGACGAGTG CCTTGAGCCT 
     5761 GAGCAACGTA GCAGGCGTCT TCTACATTCT GGTTGGCGGC TTGGGCTTGG CAATGCTGGT 
     5821 GGCTTTGATA GAGTTCTGTT ACAAGTCCAG GGCAGAGGCG AAGAGAATGA AGCTGACTTT 
     5881 TTCCGAAGCC ATAAGAAACA AAGCCAGGTT ATCCATCACT GGGAGTGTGG GAGAAAACGG 
     5941 CCGTGTGCTG ACCCCCGACT GCCCCAAGGC CGTACACACA GGAACTGCGA TTAGACAGAG 
     6001 CTCGGGATTG GCTGTCATTG CATCGGACCT ACCAACGCGT ACGCGGCCGC TCGAGCAGAA 
     6061 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     6121 TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC 
     6181 TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA 
     6241 CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 
     6301 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 
     6361 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 
     6421 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 
     6481 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 
     6541 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 
     6601 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 
     6661 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 
     6721 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 
     6781 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 
     6841 GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT 
     6901 CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA 
     6961 GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC 
     7021 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 
     7081 CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA 
     7141 TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA 
     7201 CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG 
     7261 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG 
     7321 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC 
     7381 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 
     7441 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 
     7501 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 
     7561 CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     7621 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     7681 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     7741 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     7801 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     7861 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     7921 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     7981 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     8041 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     8101 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     8161 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     8221 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     8281 GGTCATGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT 
     8341 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 
     8401 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 
     8461 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA 
     8521 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA 
     8581 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT 
     8641 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 
     8701 ACCAGCTCAC CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA 
     8761 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG 
     8821 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC 
     8881 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT 
     8941 TTCTCCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     9001 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTCCC GCGCACATTT 
     9061 CCCCGAAAAG TGCCACCTGA C
//