LOCUS       MR211067                7542 bp    DNA     circular  SYN 
DEFINITION  Hmgcr (Myc-DDK-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr)
ACCESSION   NM_008255
VERSION     NM_008255.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3699..3790 
                     /label="Myc-DDK"  
     misc_feature    3820..4405 
                     /label="PolyA signal"  
     misc_feature    3877..3896 
                     /label="Restriction Enzyme"  
     misc_feature    4681..5353 
                     /label="ColE1"  
     misc_feature    5783..6577 
                     /label="Kan/Neo"  
     misc_feature    6612..6914 
                     /label="SV40 ori"  
     misc_feature    7000..7477 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3681..3687 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3681 
                     /label="Hmgcr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTCAAGA CTTTTCCGGA TGCATGGCCT CTTCGTGGCC TCCCACCCCT 
     1081 GGGAAGTTAT TGTGGGAACA GTGACACTTA CAATCTGTAT GATGTCCATG AACATGTTCA 
     1141 CCGGCAACAA CAAGATCTGT GGCTGGAATT ATGAGTGCCC TAAATTTGAA GAGGACGTGC 
     1201 TGAGCAGCGA CATCATCATC CTGACGATAA CGCGGTGCAT CGCCATCCTG TACATTTACT 
     1261 TCCAGTTCCA GAACCTACGG CAGCTTGGGT CCAAGTACAT TCTGGGTATT GCTGGCCTCT 
     1321 TCACAATTTT CTCAAGCTTT GTCTTCAGTA CAGTCGTCAT TCATTTCCTC GACAAAGAAC 
     1381 TGACAGGCTT AAATGAAGCT TTGCCCTTTT TTCTGCTCTT GATTGACCTT TCTAGAGCGA 
     1441 GTGCATTAGC AAAGTTTGCC CTCAGTTCAA ATTCACAGGA TGAAGTAAGG GAAAATATAG 
     1501 CTCGTGGAAT GGCAATCCTG GGCCCCACAT TCACTCTTGA CGCTCTTGTG GAATGCCTTG 
     1561 TGATTGGAGT TGGCACCATG TCAGGCGTCC GCCAGCTGGA GATCATGTGC TGCTTCGGCT 
     1621 GCATGTCAGT GTTGGCCAAC TACTTTGTGT TCATGACATT CTTCCCGGCC TGTGTGTCGC 
     1681 TGGTCCTAGA GCTTTCTCGT GAAAGCCGTG AGGGTCGTCC AATTTGGCAG CTCAGCCATT 
     1741 TTGCCAGAGT TTTAGAAGAA GAAGAAAATA AACCAAACCC CGTAACCCAA AGGGTCAAGA 
     1801 TGATTATGTC TTTAGGCTTG GTCCTTGTTC ACGCTCATAG TCGCTGGATA GCTGATCCTT 
     1861 CTCCTCAGAA CAGCACAGCG GAGCAGGCTA AGGTTTCCTT GGGTCTGGAC GAAGATGTGT 
     1921 CCAAGAGAAT TGAACCAAGT GTTTCTCTCT GGCAGTTTTA TCTCTCCAAG ATGATCAGCA 
     1981 TGGACATTGA GCAAGTGATT ACCCTGAGTT TAGCCTTCCT TTTGGCTGTC AAGTATATTT 
     2041 TCTTTGAACA AGCAGAGACA GAATCGACAC TGTCATTAAA AAATCCTATC ACGTCTCCCG 
     2101 TCGTGACCTC AAAGAAAGCT CAAGACAACT GTTGTAGACG TGAGCCTCTG CTTGTGAGAA 
     2161 GGAACCAGAA GCTTTCGTCA GTAGAGGAGG ATCCAGGAGC GAACCAAGAG AGAAAAGTTG 
     2221 AGGTTATAAA ACCATTAGTG GTGGAAGCAG AGACTACAAG CAGAGCTACG TTTGTGCTTG 
     2281 GGGCTTCTGT AGCCAGCCCT CCATCCGCCC TGGGGACACA GGAGCCTGGA ATTGAACTCC 
     2341 CCATCGAGCC ACGACCTAAT GAAGAATGTC TGCAGATACT GGAGAATGCA GAGAAAGGTG 
     2401 CAAAGTTCCT TAGTGATGCA GAGATCATCC AGTTGGTCAA TGCCAAGCAT ATCCCAGCTT 
     2461 ACAAATTGGA AACTCTAATG GAAACTCATG AACGTGGTGT GTCTATTCGC CGGCAGCTCC 
     2521 TCTCCACAAA GCTTGCAGAG CCGTCCTCTC TACAGTACCT GCCTTACAGA GATTATAATT 
     2581 ATTCCTTGGT GATGGGAGCT TGCTGTGAGA ATGTGATCGG ATATATGCCC ATCCCTGTTG 
     2641 GAGTGGCAGG GCCTCTGTGC CTGGATGGGA AGGAGTACCA GGTGCCGATG GCAACGACGG 
     2701 AAGGCTGTCT TGTGGCCAGC ACTAACAGAG GCTGCAGAGC CATAAGCCTT GGTGGAGGTG 
     2761 CCAGCAGCCG GGTCCTTGCA GATGGGATGA CTCGAGGCCC AGTGGTGCGT CTTCCACGTG 
     2821 CTTGTGACTC TGCAGAAGTA AAGACCTGGC TTGAAACACC TGAAGGGTTT GCAGTGATAA 
     2881 AGGAGGCCTT TGATAGCACC AGCAGATTTG CTCGTCTACA GAAACTCCAC GTGACGATGG 
     2941 CAGGACGCAA CCTCTATATC CGTTTCCAGT CCAGGACGGG GGACGCCATG GGCATGAACA 
     3001 TGATCTCTAA GGGTACGGAG AAAGCACTGC TGAAGCTTCA GGAGTTCTTT CCTGACATGC 
     3061 AGATTCTGGC AGTCAGTGGG AACTATTGCA CCGACAAGAA GCCTGCTGCC ATAAATTGGA 
     3121 TCGAAGGACG AGGAAAGACT GTGGTTTGTG AAGCCGTCAT TCCAGCCAAG GTGGTGAGAG 
     3181 AGGTGTTAAA GACAACTACG GAAGCTATGG TTGACGTAAA CATTAACAAG AATCTTGTGG 
     3241 GCTCGGCCAT GGCTGGGAGC ATAGGCGGCT ACAACGCCCA CGCAGCAAAC ATTGTCACTG 
     3301 CTATCTACAT CGCATGTGGC CAGGATGCAG CACAGAATGT GGGGAGTTCA AACTGTATTA 
     3361 CTTTAATGGA AGCCAGTGGT CCCACAAATG AAGACTTATA TATCAGCTGC ACCATGCCAT 
     3421 CGATAGAGAT AGGAACCGTG GGTGGTGGGA CCAACCTTCT ACCTCAGCAA GCCTGCCTGC 
     3481 AGATGCTAGG TGTTCAAGGA GCATGCAAAG ACAATCCTGG AGAAAACGCA CGGCAGCTTG 
     3541 CCCGAATTGT ATGTGGCACT GTGATGGCTG GTGAGCTGTC CTTGATGGCA GCCTTGGCAG 
     3601 CAGGACATCT TGTCAGAAGT CACATGGTTC ACAACAGATC AAAGATAAAT TTACAAGATC 
     3661 TTCAAGGAAC GTGCACCAAG AAGGCAGCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3721 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3781 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3841 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3901 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3961 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4021 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4081 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4141 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4201 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4261 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4321 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4381 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4441 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4501 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4561 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4621 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4681 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4741 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4801 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4861 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4921 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4981 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5041 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5101 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5161 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5221 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5281 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5341 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5401 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5461 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5521 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5581 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5641 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5701 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5761 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5821 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5881 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5941 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6001 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6061 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6121 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6181 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6241 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6301 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6361 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6421 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6481 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6541 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6601 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6661 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6721 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6781 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6841 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6901 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6961 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7021 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7081 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7141 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7201 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7261 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7321 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7381 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7441 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7501 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//