LOCUS       MR211060                7539 bp    DNA     circular  SYN 
DEFINITION  Kif18a (Myc-DDK-tagged) - Mouse kinesin family member 18A (Kif18a)
ACCESSION   NM_139303
VERSION     NM_139303.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3696..3787 
                     /label="Myc-DDK"  
     misc_feature    3817..4402 
                     /label="PolyA signal"  
     misc_feature    3874..3893 
                     /label="Restriction Enzyme"  
     misc_feature    4678..5350 
                     /label="ColE1"  
     misc_feature    5780..6574 
                     /label="Kan/Neo"  
     misc_feature    6609..6911 
                     /label="SV40 ori"  
     misc_feature    6997..7474 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3678..3684 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3678 
                     /label="Kif18a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGCACT GAAGAAGACT TATGTCACCG CATGAAAGTA GTTGTCCGTG 
     1081 TACGTCCTGA GAACACAAAA GAAAAGGCGG TGCAGTTCTG TAAAGTGGTT CATGTAGTGG 
     1141 ATAAACATAT ACTCAGTTTT GATCCGAAAC AAGAAGAAAT CAGTTTTTTT CACAGAAAGA 
     1201 AAACTACAAA TTTTGATATT ACTAAAAGGC AAAATAAAGA TCTGAAGTTT GTATTTGATG 
     1261 CTGTCTTTGA TGAAACTTCA ACTCAAATGG AAGTTTTTGA ACACACAACC AAGCCAATCC 
     1321 TTCATAGTTT TCTGAATGGA TACAATTGCA CAGTATTTGC ATATGGTGCC ACTGGATCTG 
     1381 GGAAAACTCA CACAATGCTA GGATCAGCTG CTGAACCTGG GGTCATGTAC CTGACAATGC 
     1441 TGGACCTTTT CAAATGCATC GATGAGATTA AAGAAGAGAA AGAGTGTAGC ACTGCCGTGT 
     1501 CATACCTGGA GGTATATAAT GAACAGATCC GTGACCTCTT GACAAACTCA GGACCACTTG 
     1561 CTGTCCGGGA AGATTCCCAA AAAGGAGTGG TTGTTCAGGG TCTTACTCTA CACCAGCCTA 
     1621 AATCTTCAGA GGAAATATTA CAGCTACTAG ACAATGGAAA CAAAAACAGG ACCCAGCATC 
     1681 CCACTGACGT GAATGCAGTG TCTTCTCGTT CCCATGCTGT TTTCCAGATT TATTTGCGAC 
     1741 AACAAGACAA AACAGCGAGC ATCAATCAAA ATGTCCGTAT AGCCAAAATG TCACTCATCG 
     1801 ACCTCGCAGG ATCTGAGAGG GCCAGTGTTT CGGGTGCTAA AGGGTCACGA TTTGTAGAAG 
     1861 GCACAAATAT TAACAAATCT CTTTTAGCTC TTGGGAATGT CATCAATGCC TTAGCAAATA 
     1921 CAAAGAGAAG AAATCAGCAT ATTCCTTACA GAAATAGTAA GCTTACTCGT TTGCTGAAGG 
     1981 ATTCTCTTGG AGGAAACTGT CAAACTATAA TGATAGCTGC TGTTAGTCCT TCCTCTTTAT 
     2041 TTTATGATGA CACATATAAC ACTCTTAAGT ATGCTAATCG TGCAAAGGAA ATTAAATCTT 
     2101 CTTTGAAGAG CAATGTTCTT AATCTCAACA GTCACATATC TCAGTATGTA AAGATATGTA 
     2161 ATATGCAGAA AGCAGAGATT TTAATGTTGA AAGAAAAACT AAAGGCCTAT GAAGAACAGA 
     2221 AAGCCCTGTC TGACAGAAAT GACTGTGCTA AATTAGTACA TTCAAACCCA GAAGACAGAG 
     2281 AAACTGAAAG ATTTCAAGAA ATTCTGAACT GCTTGTTCCA GAATCGAGAA GGAATCAGGC 
     2341 AAGAGTATCT GAAGTTAGAA ATGTTACTTA AGGCAAATGC GCTAAAGTCA TCCTATCACC 
     2401 AACAGTGCCA TAAACAGATA GAGATGATGT GTTCTGAAGA CAAAGTGGAA AAGGCCACTT 
     2461 GCAAACGAGA TCACAGACTT GAAAAGTTGA AAACCAACAG CTGTTTTCTA GAGAAAAAGA 
     2521 AAGAAGAGGT GTCGAAACAA TTTGATGAAA ATACTAATTG GCTACATCGT GTTGAAAACG 
     2581 AAATGCGACT CTTGGGTCAA AATGGTGATA TTCCAGAGGC ACTCAATAAA GAGCTTCACT 
     2641 GTCACCATTT ACACCTCCAG AACAAAGAAC TGAAAACACA AATGGCACAC ATGACAGCTC 
     2701 TAGCTTGTCT CCAGGAACAG CAGCACAAGC AAACTGAGGC AGTATTGAAT GCTTTGCTTC 
     2761 CAGTACTAAG AAAACAGTAT TGGAAATTGA AAGAAACTGG CCTGTCAAAT GCTGCTTTTG 
     2821 ATTCTGACTT CAAAGATATT GAACATTTAG TAGAGAGAAA AAAGGTGGTG GCTTGGGCTG 
     2881 ACCAAACTAA TGAACATTCA AACCGAAATG ATCTTCCAGG GATTTCTCTT CTTATGACCT 
     2941 TCCCACAGCT TGAACCAATT CAGTCTATTT CTTGTTGCAC ATCTGTGAGT GATCCTAACG 
     3001 TGCTTAAACT TACTCCACAA CGAAGAACCA GAAGAAAAAT AATTCCTTCT CCCTTGAAAG 
     3061 TACAACATAC TCAGAAGTCT GCACTGTCTG AAAGTACACA GCTCAATGAT TCTTTCAGCA 
     3121 AAGAACTTCA GCCTATTGTT TATACGCCAG AGGACTGCAA AAAAGCTCAA GACCTGTTTC 
     3181 CATCCTTAAC GAGAACTTCA TCGCAGTCTG CAAATGTAAT GAATGACAAT TCCCAGAAAG 
     3241 CACTGTGTAG AATAGAGTCT CCTCTCAGCA GAACTGAGTG CAAACAGGGT TTGTACTCTA 
     3301 CATCTACTCT GTGTGATAGC ATCAGAGGCT TGAAGAATAA ATGGCCTGAA CAAGAACCAC 
     3361 TAGCAAGCAG CAAAAGCAGT GTGCACAGGA TAGAGTCTTC TTCATTTTCA ACAAAGGATT 
     3421 CTATGCCTGA ATCAGCAGGT GTGCCGTCCT ACATGGCAAT GACTACTGCT GCAAAGAGGA 
     3481 AATGGAAACA AATGAGTTCT ACATCAAATG CTTCAATCAA ATCTGATGAA AGTTGTGGAT 
     3541 TTGCCAAACG CATTCGACGG GATAATTCAA GCGTTAAGCC TATGCAAGAA AACAGACTGA 
     3601 AAGTGGGCTA TAAAAGAAAT ACCAACAAAA CAAATTCAAA CATGCTTAGA AAATTTAGAA 
     3661 GAAACACTTC AAAAGAAAAT GTACAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3721 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3781 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3841 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3901 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3961 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4021 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4081 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4141 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4201 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4261 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4321 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4381 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4441 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4501 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4561 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5161 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5221 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5281 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5341 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5401 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5461 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5521 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5581 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5641 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5701 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5761 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5821 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5881 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5941 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6001 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6061 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6121 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6181 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6241 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6301 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6361 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6421 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6481 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6541 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6601 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6661 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6721 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6781 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6841 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6901 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6961 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7021 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7081 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7141 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7201 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7261 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7321 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7381 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7441 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7501 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//