LOCUS       MR210996                7470 bp    DNA     circular  SYN 
DEFINITION  Rps6ka5 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 5 (Rps6ka5)
ACCESSION   NM_153587
VERSION     NM_153587.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3627..3718 
                     /label="Myc-DDK"  
     misc_feature    3748..4333 
                     /label="PolyA signal"  
     misc_feature    3805..3824 
                     /label="Restriction Enzyme"  
     misc_feature    4609..5281 
                     /label="ColE1"  
     misc_feature    5711..6505 
                     /label="Kan/Neo"  
     misc_feature    6540..6842 
                     /label="SV40 ori"  
     misc_feature    6928..7405 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3609..3615 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3609 
                     /label="Rps6ka5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGGGGAG GGCGGCGGCA GCGGCGGCGC GGGCACCAGC GGGGACAGCG 
     1081 GCGACGGCGG CGAGCAGCTC CTTACTGTCA AGCACGAGCT GCGGACCGCC AACTTAACAG 
     1141 GACACGCAGA GAAGGTGGGG ATTGAAAACT TTGAGCTGCT GAAGGTCCTA GGAACTGGAG 
     1201 CTTATGGGAA AGTATTTCTA GTTCGTAAAA TAAGCGGCCA TGATGCTGGA AAGCTGTATG 
     1261 CTATGAAGGT TTTAAAAAAG GCAACAATAG TTCAGAAGGC TAAAACTACA GAGCATACAA 
     1321 GGACAGAACG ACAGGTTCTG GAGCACATTA GACAGTCGCC ATTTTTGGTG ACATTGCACT 
     1381 ATGCTTTCCA GACAGAGACC AAGCTTCATC TCATCTTAGA TTATATAAAT GGTGGAGAAC 
     1441 TTTTTACTCA TCTTTCTCAA AGAGAGCGTT TCACAGAGCA CGAAGTGCAG ATCTATGTTG 
     1501 GAGAGATTGT GCTTGCCCTG GAACACCTGC ACAAGTTGGG GATTATATAC CGTGACATTA 
     1561 AGCTTGAAAA TATTCTCCTG GATTCTAACG GCCATGTGGT GCTGACAGAT TTCGGTCTGA 
     1621 GTAAGGAGTT TGTGGCCGAT GAAACTGAAA GAGCATATTC TTTTTGTGGA ACTATTGAAT 
     1681 ACATGGCTCC AGATATTGTC AGAGGGGGTG ATTCGGGACA CGACAAGGCA GTTGACTGGT 
     1741 GGAGCTTAGG TGTTCTAATG TATGAGCTAC TAACTGGAGC ATCTCCTTTC ACTGTCGATG 
     1801 GAGAAAAAAA TTCCCAGGCT GAGATATCTA GGAGAATTTT AAAAAGTGAG CCTCCGTATC 
     1861 CCCAGGAAAT GAGCACAGTA GCTAAAGACC TACTTCAGCG TCTCTTGATG AAAGATCCCA 
     1921 AGAAGCGATT GGGATGTGGC CCACGGGATG CAGAAGAAAT CAAAGAACAT CTCTTCTTTG 
     1981 AGAAGATAAA GTGGGATGAC TTAGCTGCCA AAAAAGTGCC TGCGCCATTC AAGCCGGTGA 
     2041 TCCGGGATGA GTTAGATGTG AGTAACTTCG CTGAAGAGTT CACAGAGATG GACCCCACCT 
     2101 ACTCTCCCGC TGCCTTGCCA CAGAGCTCCG AGCGACTGTT TCAGGGCTAT TCCTTCGTTG 
     2161 CTCCATCTAT TCTCTTCAAG CGTAACGCAG CTGTCATAGA TCCTCTTCAG TTCCATATGG 
     2221 GAGTTGACCG TCCTGGAGTG ACAAATGTTG CCAGGAGTGC AATGATGAAG GACTCACCCT 
     2281 TCTATCAACA CTATGACCTA GATCTGAAGG ACAAACCTTT GGGAGAAGGA AGTTTTTCAA 
     2341 TTTGTCGCAA GTGTGTACAC AAAAAAACTA ACCAAGCATT TGCTGTCAAA ATAATCAGCA 
     2401 AAAGGATGGA AGCCAATACT CAGAAAGAAA TAACAGCTCT GAAACTCTGC GAAGGACACC 
     2461 CCAATATTGT GAAACTACAT GAAGTTTTTC ACGATCAGGT GGCAGCGTCT GCTCAGCCCC 
     2521 CAGGACAGGT TGTTCTATGC TCTCTTCTAC TCTTAGCTCT GCTTTTCAAC AGAAGTCTCA 
     2581 CACGGAAGCC TGTCACTTGG ACATGGCTGG TCCATAGCAC CTCTCAGCTC CCTCCATTGC 
     2641 CACCTCCCAT GCCTGAAATT GTGCTATTTA TTCTTCTCTC TGATAATGGA CAGCTTCATA 
     2701 CGTTTCTTGT GATGGAACTT CTGAATGGCG GAGAGCTGTT CGAGCGCATC AAGAGAAAGA 
     2761 AGCACTTCAG CGAGACTGAG GCCAGCTACA TCATGCGGAA GCTTGTGTCA GCTGTGAGCC 
     2821 ACATGCACGA TGTAGGAGTG GTGCACAGAG ATCTGAAGCC TGAGAATCTG TTGTTCACTG 
     2881 ATGAAAACGA CAATTTGGAA ATTAAAGTGA TTGACTTTGG GTTTGCACGC CTCAAGCCAC 
     2941 CTGACAATCA GCCCCTCAAG ACCCCGTGCT TCACCCTCCA CTACGCAGCA CCGGAGCTCT 
     3001 TGACGCACAA TGGCTACGAT GAGTCCTGTG ACCTGTGGAG CTTGGGTGTC ATCCTGTATA 
     3061 CAATGCTGTC AGGGCAGGTG CCATTCCAGT CTCATGACAG AAGTTTAACA TGCACCAGTG 
     3121 CAGTGGAAAT CATGAAGAAA ATTAAAAAGG GAGACTTCTC CTTTGAAGGA GAAGCGTGGA 
     3181 AGAATGTATC CCAAGAGGCT AAAGATTTGA TTCAAGGGCT TCTTACAGTT GATCCAAATA 
     3241 AAAGGCTGAA AATGTCTGGT TTGAGATACA ATGAATGGCT TCAGGATGGC AGTCAGCTGT 
     3301 CCTCCAATCC TCTGATGACT CCAGACATTC TGGGATCGTC AGGAGCAGCT GTGCACACCT 
     3361 GTGTGAAAGC AACCTTTCAT GCCTTTAACA AGTATAAGAG AGAAGGATTT TGCCTGCAGA 
     3421 ATGTGGATAA GGCCCCTTTG GCTAAGAGGA GGAAAATGAA GAGGACCAGC ACCAGCACAG 
     3481 AGACTCGCAG CAGCTCCAGC GAGAGCTCCC GCTCCTCCTC CTCCCAGTCC CACGGCAAGA 
     3541 CAACGCCTAC CAAGACGCTG CAGCCCAGCA ATCCCACCGA AGGCAGTAAC CCAGACACCC 
     3601 TCTTCCAGTT CTCAGACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3661 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3721 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3781 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3841 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3901 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3961 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4021 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4081 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4141 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4201 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4261 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4321 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4381 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4441 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4501 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4561 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4621 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4681 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4741 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4801 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4861 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4921 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4981 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5041 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5101 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5161 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5221 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5281 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5341 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5401 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5461 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5521 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5581 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5641 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5701 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5761 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5821 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5881 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5941 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6001 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6061 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6121 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6181 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6241 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6301 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6361 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6421 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6481 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6541 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6601 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6661 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6721 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6781 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6841 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6901 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6961 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7021 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7081 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7141 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7201 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7261 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7321 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7381 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7441 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//