LOCUS       MR210971                7455 bp    DNA     circular  SYN 
DEFINITION  Usp5 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5)
ACCESSION   NM_013700
VERSION     NM_013700.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3612..3703 
                     /label="Myc-DDK"  
     misc_feature    3733..4318 
                     /label="PolyA signal"  
     misc_feature    3790..3809 
                     /label="Restriction Enzyme"  
     misc_feature    4594..5266 
                     /label="ColE1"  
     misc_feature    5696..6490 
                     /label="Kan/Neo"  
     misc_feature    6525..6827 
                     /label="SV40 ori"  
     misc_feature    6913..7390 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3594..3600 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3594 
                     /label="Usp5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGCTG AGTGAAGAGG CGCTGCTGTC AGTGTTACCG ACGATCCGTG 
     1081 TCCCCAAGGC GGGAGACCGG GTCCATAAAG ACGAGTGCGC TTTCTCTTTC GACACGCCGG 
     1141 AGTCTGAGGG TGGCCTCTAT ATCTGCATGA ACACATTCCT GGGATTCGGG AAGCAGTATG 
     1201 TGGAGAGACA CTTCAACAAG ACAGGCCAGC GTGTCTACCT GCACCTCCGG AGGACCCGGC 
     1261 GACCGAAAGA AGAGGACACC AGTGCAGGCA CTGGAGACCC ACCTCGGAAG AAGCCCACCC 
     1321 GGCTGGCCAT TGGTGTTGAA GGAGGGTTTG ACCTCACCGA GGACAAGTTT GAATTTGACG 
     1381 AGGATGTGAA GATTGTCATT TTGCCCGATT ACCTGGAGAT CGCTCGGGAT GGGTTGGGGG 
     1441 GGCTTCCTGA CATTGTCAGA GATCGGGTGA CCAGTGCTGT GGAAGCCCTA CTTTCTGCTG 
     1501 ACTCAGCCTC TCGAAAGCAG GAGGTCCAGG CTTGGGATGG GGAAGTCCGT CAGGTGTCCA 
     1561 AGCATGCCTT CAACCTCAAG CAGTTGGACA ACCCTGCTCG GATCCCTCCC TGTGGCTGGA 
     1621 AGTGCTCCAA GTGCGACATG CGCGAGAACC TGTGGCTCAA CCTGACAGAC GGCTCCATCC 
     1681 TCTGTGGACG GCGCTACTTC GATGGCAGTG GGGGCAACAA CCATGCTGTG GAGCACTACA 
     1741 GGGAGACTGG CTACCCCTTA GCTGTTAAGC TGGGCACCAT CACCCCGGAT GGAGCTGACG 
     1801 TGTATTCTTA CGACGAGGAT GACATGGTTC TGGACCCCAG CCTGGCAGAG CACTTGTCTC 
     1861 ACTTTGGCAT CGACATGCTG AAGATGCAGA AGACGGACAA GACGATGACT GAGCTGGAGA 
     1921 TAGACATGAA CCAGCGGATT GGTGAATGGG AGTTGATCCA GGAGTCAGGC GTGCCACTCA 
     1981 AGCCTCTGTT TGGGCCTGGT TACACGGGCA TCCGGAACCT GGGTAACAGC TGCTATCTCA 
     2041 ACTCCGTGGT CCAGGTGCTC TTCAGCATCC CTGACTTTCA GAGGAAGTAT GTGGATAAAT 
     2101 TGGAGAAGAT CTTCCAGAAT GCCCCAACGG ACCCCACCCA AGACTTCAGC ACCCAGGTGG 
     2161 CCAAGCTGGG GCATGGCCTT CTCTCTGGAG AATACTCTAA GCCAGCACTG GAGTCAGGTG 
     2221 ATGGGGAGCA GGTACCAGAA CAAAAGGAAG TTCAAGATGG CATTGCCCCT CGAATGTTCA 
     2281 AGGCCCTCAT TGGCAAGGGC CACCCTGAGT TCTCCACTAA TCGGCAGCAG GATGCCCAGG 
     2341 AATTCTTCCT ACATCTTATC AACATGGTGG AGAGGAATTG CCGGAGTTCT GAAAATCCAA 
     2401 ATGAAGTGTT CCGCTTTTTG GTGGAAGAAA AGATCAAATG CCTGGCCACA GAGAAGGTTA 
     2461 AGTACACTCA GCGAGTTGAC TACATCATGC AGTTGCCTGT ACCCATGGAT GCAGCCCTTA 
     2521 ACAAAGAGGA GCTTTTAGAG TATGAAGAGA AGAAGCGGCA AGCTGAAGAG GAGAAAGTGC 
     2581 CACTGCCAGA ACTGGTTCGG GCCCAGGTGC CCTTCAGCTC CTGCCTGGAA GCCTACGGGG 
     2641 CCCCTGAGCA GGTGGACGAC TTCTGGAGTA CAGCTCTACA GGCGAAGTCG GTCGCTGTCA 
     2701 AGACCACGCG CTTTGCCTCC TTCCCCGACT ACCTGGTCAT CCAGATCAAG AAGTTCACCT 
     2761 TTGGCTTAGA CTGGGTGCCC AAGAAGCTGG ATGTGTCCAT TGAGATGCCA GAGGAGCTCG 
     2821 ATATCTCCCA GCTGAGGGGC ACAGGGCTAC AGCCGGGAGA GGAGGAGCTG CCTGACATTG 
     2881 CCCCACCCCT GGTCACTCCG GATGAGCCCA AAGGTAGCCT TGGTTTCTAT GGCAACGAAG 
     2941 ACGAAGACTC CTTCTGCTCC CCTCACTTCT CCTCTCCGAC ATCACCCATG CTGGACGAAT 
     3001 CCGTCATCAT ACAGTTGGTG GAAATGGGCT TCCCTATGGA TGCCTGCCGG AAAGCTGTGT 
     3061 ACTATACGGG AAACAGCGGG GCCGAAGCAG CCATGAACTG GGTCATGTCA CACATGGATG 
     3121 ATCCAGATTT TGCAAACCCC CTCATCCTGC CTGGCTCCAG TGGGCCTGGC TCCACAAGTG 
     3181 CAGCAGCTGA CCCCCCGCCG GAGGACTGTG TGACCACCAT AGTCTCCATG GGCTTCTCCA 
     3241 GGGACCAGGC CCTGAAAGCT CTGCGGGCCA CGAACAATAG CTTAGAGCGG GCTGTAGACT 
     3301 GGATCTTCAG CCACATTGAT GACCTGGACG CAGAAGCTGC TATGGACATC TCAGAGGGAC 
     3361 GCTCAGCGGC TGAGTCCATC TCCGAGTCTG TGCCAGTGGG ACCTAAAGTC CGGGATGGTC 
     3421 CTGGAAAGTA TCAGCTCTTT GCCTTCATTA GTCACATGGG CACATCTACT ATGTGTGGTC 
     3481 ACTATGTCTG CCATATCAAG AAGGAAGGCA GGTGGGTGAT CTACAATGAC CAGAAAGTGT 
     3541 GTGCCTCTGA AAAGCCACCC AAGGACCTGG GCTACATCTA CTTCTACCAG AGAGTGGTCA 
     3601 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3661 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3721 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3781 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3841 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3901 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3961 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4021 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4081 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4141 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4201 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4261 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4321 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4381 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4441 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4501 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4561 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4621 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4681 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4741 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4801 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4861 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4921 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4981 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5041 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5101 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5161 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5221 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5281 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5341 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5401 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5461 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5521 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5581 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5641 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5701 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5761 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5821 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5881 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5941 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6001 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6061 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6121 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6181 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6241 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6301 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6361 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6421 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6481 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6541 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6601 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6661 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6721 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6781 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6841 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6901 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6961 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7021 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7081 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7141 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7201 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7261 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7321 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7381 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7441 TTTAACGCGA ATTTT
//