LOCUS       MR210833                7356 bp    DNA     circular  SYN 
DEFINITION  Usp16 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 16 (Usp16)
ACCESSION   NM_024258
VERSION     NM_024258.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3513..3604 
                     /label="Myc-DDK"  
     misc_feature    3634..4219 
                     /label="PolyA signal"  
     misc_feature    3691..3710 
                     /label="Restriction Enzyme"  
     misc_feature    4495..5167 
                     /label="ColE1"  
     misc_feature    5597..6391 
                     /label="Kan/Neo"  
     misc_feature    6426..6728 
                     /label="SV40 ori"  
     misc_feature    6814..7291 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3495..3501 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3495 
                     /label="Usp16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGAAA CGGACCAAGG GGAGAAGTGC TCCAGACACG GTGGCCTCAG 
     1081 AGTCTGCAGA ACCAGTGTGC AGACACCTTA GAAAAGGGTT GGAACAAGGT AATTTGAAAA 
     1141 AAGCTTTAGT AAATGTGGAG TGGAATATCT GCCAAGACTG TAAGACTGAC AATAAAGTGA 
     1201 AAGATAAACC TGAGGAGGAA GCAGAAGACC CTTCGGTTTG GCTCTGTCTT AAATGTGGCC 
     1261 ATCAGGGCTG TGGCAGAGAT TCTCAGGAGC AGCATGCCTT GAAGCACTAC ACGACACCGA 
     1321 GATCCGAGCC TCACTACCTG GTGCTCAGTC TGGACAACTG GAGCGTCTGG TGCTACAAGT 
     1381 GTGACGAGGA AGTCAAGTAC TGTAGCTCAA ACCGATTGGG CCAAGTGGTT GATTATGTTA 
     1441 GAAAACAAGC TGGCGTAAGA ACTTCAAAAC CAGCAGAGAA AAATAATGGA CACATTGAGC 
     1501 TCGAAAATAA AAAATTGGAG AAAGAGAGTA AAAATGAACA AGAGAAAGAG AAATCGGAAA 
     1561 ACCTGGCTAA AGAAACTATT CCCATGGACT CTGCTTCCCA GATAACTGTG AAAGGACTCA 
     1621 GTAATTTGGG GAATACTTGT TTCTTCAATG CAGTTATGCA GAACTTGTCA CAAACGCCAG 
     1681 TGCTTAGAGA ACTACTAAAA GAAGTGAAGA TGTCTGGAAC GATTGTAAAA ATAGAGCCAC 
     1741 CTGATCTGGC ACTAACAGAA CCTTTAGAAG TAAACCTCGA GCCTCCAGGT CCTCTTACTT 
     1801 TAGCCATGAG CCAGTTTCTC AGTGAGATGC AAGAGAACAA AAAGCGAGTT GTGACACCTA 
     1861 AAGAGCTCTT TTCTCAGGTC TGTAAAAAAG CAACACGTTT TAAAGGGTAC CAGCAACAAG 
     1921 ACAGCCAGGA GCTGCTTCGC TACCTACTGG ATGGGATGAG AGCGGAAGAA CACCAAAGAG 
     1981 TGAGTAAAGG AATTCTTAAA GCATTTGGTA ATTCTACTGA AAAATTGGAT GAAGAAGTAA 
     2041 AAAATAAAGT TAAAGATTAT GAAAAGAAAA AGGCAATCCC GAGTTTTGTG GACCGCATCT 
     2101 TTGGTGGCGA GCTGACTAGT ACGATCATGT GTGATGAATG CAGGACTGTC TCCTTAGTGC 
     2161 ATGAATCGTT CCTTGATTTG TCTCTTCCAG TTTTAGATGA TCAGAGTGGT AAGAAAAGTA 
     2221 TAAATGATAA AAATGTGAAA ATGACAATGG AGGAAGAAGA TAAAGACAGT GAGGAAGAGA 
     2281 AAGATGACAG CTACATGAAA TCAAGGAGCG ATCTTCCGTC AGGGACAAGC AAGCACCTAC 
     2341 AGAAAAAGGC AAAGAAGCAG GCCAAAAAGC AGGCCAAGAA CCAACGAAGG CAACAAAAAA 
     2401 TTCAAGAAAG ATTTCTTCAC TTCAATGAGC TCTGCGCCAC TGACTACACG GAAGACAATG 
     2461 AACGTGAAGC TGACACAGCA CTTGCGGGAG AAGTGGAAGT GGATACCGAC TCCACCCATG 
     2521 GTTCTCAAGA GGAGGCCACA CAGATAGAGC TGTCTGTTAA CCAGAAGGAT TTGGATGGCC 
     2581 AAGAGAGCAT GATAGAAAGG ACACCTGATG TGCAGGAAAG CCCAGAGGAC CTAGGAGTGA 
     2641 AAAGTGCTAA CACCGAGAGT GATCTGGGGA TTGTGACGCC TGCTCCTGAA TGTCCTAGGG 
     2701 ATTTCAATGG TGCCTTCCTG GAAGAAAGGA CCAGTGGAGA ACTAGACATT ATCAATGGTT 
     2761 TAAAAAACCT TAATTTGAAT GCTGCTGTTG ATCCTGATGA AATAAATATA GAGATTCCGA 
     2821 ATGACAGTCA TTCTGCACCC AAGGTATATG AGGTCATGAA CGAGGACCCA GAAACTGCTT 
     2881 TCTGTACCCT CGCGAACCGA GAAGCGTTTA GTACTGATGA GTGTTCCATT CAACATTGCT 
     2941 TATATCAGTT CACCCGGAAT GAGAAACTTC AAGATGCCAA TAAACTGCTT TGTGAAGTGT 
     3001 GTTCAAGACG GCAGTGTAAT GGACCAAAGG CAAATATAAA AGGTGACAGG AGACATGTTT 
     3061 ACACCAATGC CAAGAAGCAG ATGCTGGTCT CCCTCGCGCC TCCTGTCCTC ACTCTGCATT 
     3121 TAAAGCGATT CCAGCAGGCT GGTTTTAACC TGCGCAAAGT TAACAAACAC ATAAAGTTTC 
     3181 CAGAAATCTT AGATTTGGCT CCTTTTTGTA CCCTTAAATG TAAGAATGTT GCTGAAGAAA 
     3241 GTACACGAGT GCTGTATTCC TTATATGGAG TTGTTGAACA CAGTGGTACT ATGAGGTCAG 
     3301 GGCATTACAC TGCCTATGCG AAGGAGAGAA CTGCAAGCTG TCACCTCTCC AATCTTGTTC 
     3361 TTCACGGTGA CATTCCACAA GATTGTGAAA TGGAATCAAC CAAAGGGCAG TGGTTTCACA 
     3421 TCAGCGATAC ACATGTGCAA GCTGTGCCTA TAACTAAAGT ACTGAACTCA CAAGCATATC 
     3481 TCCTATTTTA TGAGAGAATA CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3541 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3601 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3661 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3721 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3781 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3841 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3901 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3961 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4021 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4081 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4141 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4201 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4261 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4321 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4381 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4441 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4501 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4561 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4621 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4681 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4741 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4801 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4861 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4921 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4981 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5041 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5101 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5161 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5221 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5281 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5341 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5401 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5461 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5521 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5581 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5641 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5701 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5761 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5821 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5881 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5941 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6001 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6061 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6121 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6181 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6241 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6301 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6361 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6421 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6481 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6541 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6601 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6661 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6721 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6781 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6841 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6901 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6961 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7021 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7081 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7141 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7201 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7261 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7321 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//