LOCUS       MR210707                7269 bp    DNA     circular  SYN 
DEFINITION  Vps35 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 35 (Vps35)
ACCESSION   NM_022997
VERSION     NM_022997.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3426..3517 
                     /label="Myc-DDK"  
     misc_feature    3547..4132 
                     /label="PolyA signal"  
     misc_feature    3604..3623 
                     /label="Restriction Enzyme"  
     misc_feature    4408..5080 
                     /label="ColE1"  
     misc_feature    5510..6304 
                     /label="Kan/Neo"  
     misc_feature    6339..6641 
                     /label="SV40 ori"  
     misc_feature    6727..7204 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3408..3414 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3408 
                     /label="Vps35"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTACAACA CAGCAGTCAC CCCAGGATGA GCAGGAAAAA CTTTTGGATG 
     1081 AAGCCATCCA GGCTGTGAAG GTTCAGTCAT TCCAGATGAA AAGATGCCTG GACAAAAACA 
     1141 AGCTGATGGA TGCTCTGAAG CATGCCTCCA ATATGCTTGG AGAGCTCCGG ACCTCTATGC 
     1201 TGTCACCAAA GAGTTACTAT GAACTTTATA TGGCTATTTC TGATGAACTG CACTACTTGG 
     1261 AGGTCTACCT GACTGATGAA TTTGCTAAAG GAAGAAAGGT GGCAGATCTC TATGAACTTG 
     1321 TACAGTACGC GGGAAACATT ATTCCAAGGC TTTATCTCTT GATCACAGTT GGAGTTGTAT 
     1381 ATGTCAAGTC ATTTCCTCAA TCCAGGAAAG ATATTTTGAA AGATTTGGTA GAAATGTGCC 
     1441 GTGGTGTGCA GCATCCGCTA AGGGGTTTGT TTCTTCGAAA TTACCTTCTT CAGTGTACTA 
     1501 GGAACATTTT ACCTGATGAA GGAGAGCCAA CAGATGAAGA AACAACTGGT GATATCAGTG 
     1561 ATTCCATGGA TTTTGTACTA CTCAACTTTG CAGAAATGAA TAAGCTCTGG GTGCGGATGC 
     1621 AGCATCAAGG ACATAGTCGA GATAGAGAAA AAAGAGAACG AGAGAGACAA GAACTGAGAA 
     1681 TTTTAGTGGG AACAAATTTG GTGCGCCTTA GTCAGTTGGA AGGTGTAAAT GTGGAACGTT 
     1741 ACAAACAGAT TGTTCTAACA GGCATTCTGG AGCAGGTTGT GAATTGTAGG GATGCTCTGG 
     1801 CTCAGGAGTA TCTCATGGAG TGCATCATTC AGGTTTTTCC TGATGAGTTT CATCTTCAGA 
     1861 CTTTGAATCC TTTTCTTAGA GCTTGTGCTG AATTACACCA AAATGTAAAT GTGAAAAACA 
     1921 TAATCATTGC TTTAATTGAC AGATTAGCTT TATTTGCTCA TCGTGAAGAT GGACCCGGAA 
     1981 TTCCAGCTGA GATTAAACTT TTTGACATAT TTTCACAACA GGTGGCTACA GTCATACAGT 
     2041 CCAGACAAGA CATGCCATCA GAGGATGTTG TATCTTTACA AGTCTCTCTC ATTAATCTTG 
     2101 CTATGAAATG TTACCCTGAT CGTGTGGACT ACGTCGATAA AGTTCTAGAA ACAACAGTGG 
     2161 AGATATTCAA TAAGCTTAAC CTTGAACATA TTGCTACCAG TAGTGCAGTT TCAAAGGAGC 
     2221 TTACCAGACT TTTGAAGATA CCTGTTGATA CTTACAACAA TATCTTAACA GTATTAAAGT 
     2281 TAAAGCATTT CCACCCACTT TTTGAGTACT TTGACTACGA GTCCAGAAAG AGCATGAGCT 
     2341 GTTATGTGCT TAGTAATGTT CTGGATTATA ACACAGAAAT CGTCTCTCAG GACCAGGTAG 
     2401 ATTCCATAAT GAATTTGGTG TCCACATTGA TTCAGGATCA GCCAGACCAA CCTGTAGAAG 
     2461 ACCCTGATCC AGAAGACTTC GCTGATGAAC AGAGCCTTGT TGGCCGATTT ATTCATCTGC 
     2521 TACGTTCTGA TGATCCTGAC CAGCAGTATT TGATTTTGAA TACGGCACGA AAACATTTTG 
     2581 GGGCTGGTGG AAATCAGCGG ATTCGCTTCA CACTGCCACC TTTGGTATTT GCAGCTTACC 
     2641 AGTTGGCTTT TCGATACAAA GAGAATTCCC AAATGGATGA CAAGTGGGAA AAGAAATGCC 
     2701 AGAAGATATT TTCATTTGCT CACCAGACTA TCAGTGCTTT GATTAAAGCT GAGCTGGCTG 
     2761 AATTACCACT GAGACTTTTT CTTCAAGGAG CATTAGCTGC TGGAGAAATT GGCTTTGAAA 
     2821 ATCATGAAAC AGTAGCATAT GAATTTATGT CCCAGGCATT TTCTCTATAT GAAGATGAAA 
     2881 TCAGTGATTC TAAAGCACAG CTGGCTGCTA TCACCTTGAT CATCGGTACT TTTGAGAGGA 
     2941 TGAAATGCTT CAGTGAAGAG AATCATGAAC CCTTGAGAAC TCAGTGTGCA CTTGCTGCAT 
     3001 CAAAACTTCT GAAAAAACCA GATCAAGGCC GAGCCGTGAG CACATGTGCG CATCTCTTTT 
     3061 GGTCTGGCCG AAACACAGAC AAAAATGGGG AAGAGCTTCA TGGAGGTAAA AGGGTCATGG 
     3121 AGTGCCTAAA GAAGGCACTA AAAATAGCAA ATCAGTGCAT GGACCCCTCT CTACAAGTTC 
     3181 AGCTCTTTAT AGAGATTCTG AACAGGTACA TCTATTTCTA TGAAAAAGAA AATGATGCGG 
     3241 TAACCATTCA AGTCTTGAAC CAACTTATTC AAAAGATTCG AGAAGATCTC CCAAATCTTG 
     3301 AGTCCAGTGA AGAAACAGAA CAAATAAACA AGCATTTTCA CAACACATTG GAGCACTTGC 
     3361 GCTCAAGACG GGAATCACCA GAGTCTGAGG GGCCAATCTA TGAAGGTCTC ATCCTTACGC 
     3421 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3481 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3541 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3601 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3661 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3721 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3781 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3841 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3901 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3961 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4021 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4081 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4141 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4201 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4261 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4321 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4381 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4441 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4501 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4561 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4621 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4681 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4741 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4801 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4861 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4921 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4981 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5041 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5101 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5161 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5221 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5281 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5341 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5401 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5461 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5521 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5581 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5641 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5701 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5761 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5821 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5881 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5941 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6001 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6061 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6121 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6181 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6241 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6301 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6361 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6421 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6481 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6541 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6601 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6661 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6721 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6781 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6841 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6901 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6961 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7021 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7081 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7141 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7201 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7261 GCGAATTTT
//