LOCUS       MR210537                7176 bp    DNA     circular  SYN 
DEFINITION  Cd248 (Myc-DDK-tagged) - Mouse CD248 antigen, endosialin (Cd248)
ACCESSION   NM_054042
VERSION     NM_054042.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3333..3424 
                     /label="Myc-DDK"  
     misc_feature    3454..4039 
                     /label="PolyA signal"  
     misc_feature    3511..3530 
                     /label="Restriction Enzyme"  
     misc_feature    4315..4987 
                     /label="ColE1"  
     misc_feature    5417..6211 
                     /label="Kan/Neo"  
     misc_feature    6246..6548 
                     /label="SV40 ori"  
     misc_feature    6634..7111 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3315..3321 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3315 
                     /label="Cd248"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCTGCGC CTGCTGCTGG CCTGGGTGGC CGCGGTGCCC GCACTGGGCC 
     1081 AGGTCCCCTG GACGCCGGAG CCTCGAGCCG CGTGCGGCCC CAGCAGCTGC TACGCGCTCT 
     1141 TTCCCCGGCG CCGCACATTC CTGGAAGCTT GGCGGGCGTG CCGCGAATTG GGGGGCAACC 
     1201 TGGCCACACC GCGGACCCCA GAGGAGGCCC AGCGTGTGGA CAGCCTGGTG GGGGTCGGGC 
     1261 CGGCCAACGG GCTGCTATGG ATTGGGTTGC AGCGGCAGGC TAGGCAATGC CAGCCGCAGC 
     1321 GCCCACTGCG GGGCTTCATA TGGACCACGG GAGACCAGGA CACCGCCTTC ACCAACTGGG 
     1381 CCCAGCCGGC TACGGAAGGA CCCTGCCCAG CCCAGCGCTG TGCAGCCCTT GAGGCCAGCG 
     1441 GAGAGCATCG CTGGCTCGAA GGCTCGTGCA CACTGGCTGT CGATGGCTAC CTCTGCCAGT 
     1501 TTGGTTTTGA GGGTGCCTGC CCTGCCTTGC CGCTTGAGGT GGGTCAGGCC GGTCCCGCTG 
     1561 TCTACACCAC ACCCTTCAAC CTGGTTTCCA GCGAGTTCGA ATGGCTGCCC TTTGGCTCCG 
     1621 TGGCAGCTGT GCAGTGCCAA GCTGGCAGGG GAGCTTCTCT GCTGTGCGTG AAACAGCCTT 
     1681 CAGGTGGCGT GGGCTGGTCC CAGACTGGCC CGCTGTGCCC AGGGACTGGC TGTGGTCCTG 
     1741 ACAATGGGGG TTGCGAACAT GAGTGTGTGG AAGAGGTGGA CGGTGCTGTG TCCTGCCGCT 
     1801 GCAGTGAAGG CTTCCGTCTA GCAGCAGATG GGCACAGTTG TGAAGACCCC TGTGCCCAGG 
     1861 CCCCCTGTGA GCAGCAGTGT GAACCTGGAG GGCCACAAGG CTATAGCTGC CACTGTCGCC 
     1921 TTGGCTTCCG GCCAGCTGAG GATGATCCAC ACCGCTGCGT GGACACGGAT GAGTGCCAGA 
     1981 TTGCTGGTGT GTGCCAGCAG ATGTGTGTCA ACTATGTTGG TGGCTTTGAG TGTTACTGCA 
     2041 GCGAGGGTCA CGAGCTTGAG GCAGATGGTA TCAGCTGTAG CCCTGCAGGA GCCATGGGTG 
     2101 CCCAGGCTTC CCAGGATCTC AGAGATGAGT TGCTGGATGA TGGAGAAGAA GGGGAGGATG 
     2161 AAGAGGAGCC CTGGGAGGAC TTTGATGGCA CCTGGACAGA GGAACAGGGG ATCCTATGGC 
     2221 TGGCACCTAC ACATCCACCT GACTTTGGCC TGCCCTATAG GCCCAACTTC CCACAGGATG 
     2281 GAGAGCCTCA GAGATTGCAC CTGGAGCCTA CCTGGCCACC CCCACTTAGT GCCCCCAGGG 
     2341 GCCCCTACCA CTCCTCAGTG GTGTCTGCCA CACGGCCCAT GGTGATCTCT GCCACTCGAC 
     2401 CCACACTACC TTCTGCCCAC AAGACCTCTG TTATTTCAGC TACACGCCCA CCCCTGAGCC 
     2461 CTGTCCACCC ACCTGCCATG GCCCCTGCCA CACCTCCAGC TGTGTTCTCT GAGCACCAGA 
     2521 TCCCCAAAAT CAAGGCCAAT TATCCAGACC TGCCTTTTGG CCACAAGCCT GGGATAACCT 
     2581 CGGCCACTCA CCCAGCACGG TCTCCTCCGT ACCAGCCCCC CATTATCTCA ACCAACTATC 
     2641 CCCAAGTCTT CCCTCCCCAC CAGGCCCCTA TGTCTCCAGA TACCCACACT ATCACTTATT 
     2701 TGCCTCCAGT CCCCCCTCAC CTTGATCCTG GGGATACCAC TTCTAAAGCC CATCAACACC 
     2761 CTTTGCTCCC AGATGCTCCA GGTATCAGAA CCCAGGCCCC CCAGCTTTCT GTCTCAGCTC 
     2821 TCCAGCCCCC TCTTCCTACC AACTCCAGGT CTTCTGTCCA TGAAACTCCT GTGCCTGCTG 
     2881 CCAACCAGCC CCCAGCCTTC CCTTCTTCTC CCCTCCCCCC TCAGAGGCCC ACTAACCAGA 
     2941 CCTCATCTAT CAGCCCTACA CATTCCTATT CCAGAGCCCC TCTAGTCCCA AGGGAAGGAG 
     3001 TTCCCAGTCC CAAATCAGTG CCACAGCTGC CCTCGGTGCC CTCCACAGCA GCTCCAACAG 
     3061 CCCTGGCAGA GTCAGGTCTT GCAGGCCAAA GCCAAAGGGA TGACCGCTGG CTGCTGGTGG 
     3121 CACTCCTGGT GCCAACATGT GTCTTCTTGG TGGTGCTGCT TGCCCTGGGC ATTGTGTACT 
     3181 GCACTCGCTG TGGCTCCCAC GCACCCAACA AGCGGATCAC GGACTGCTAT CGCTGGGTCA 
     3241 CACATGCTGG GAACAAGAGC TCAACAGAAC CCATGCCCCC CAGAGGCAGC CTTACAGGGG 
     3301 TACAGACCTG TAGAACCAGT GTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3361 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3421 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3481 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3541 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3601 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3661 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3721 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3781 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3841 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3901 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3961 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4021 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4081 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4141 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4201 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4261 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4321 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4381 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4441 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4501 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4561 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4621 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4681 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4741 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4801 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4861 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4921 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4981 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5041 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5101 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5161 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5221 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5281 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5341 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5401 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5461 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5521 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5581 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5641 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5701 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5761 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5821 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5881 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5941 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6001 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6061 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6121 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6181 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6241 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6301 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6361 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6421 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6481 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6541 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6601 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6661 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6721 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6781 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6841 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6901 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6961 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7021 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7081 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7141 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//