LOCUS       MR210507                7158 bp    DNA     circular  SYN 
DEFINITION  Lipe (Myc-DDK-tagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 2
ACCESSION   NM_001039507
VERSION     NM_001039507.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3315..3406 
                     /label="Myc-DDK"  
     misc_feature    3436..4021 
                     /label="PolyA signal"  
     misc_feature    3493..3512 
                     /label="Restriction Enzyme"  
     misc_feature    4297..4969 
                     /label="ColE1"  
     misc_feature    5399..6193 
                     /label="Kan/Neo"  
     misc_feature    6228..6530 
                     /label="SV40 ori"  
     misc_feature    6616..7093 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3297..3303 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3297 
                     /label="Lipe"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTTACGC ACGATGACAC AGTCGCTGGT GACACTCGCA GAAGACAATA 
     1081 TGGCCTTCTT CTCAAGCCAG GGCCCAGGAG AGACAGCACG GCGGCTGTCT AATGTCTTTG 
     1141 CAGGTGTTCG GGAACAGGCA CTGGGGCTGG AACCAACCCT AGGCCAACTG TTGGGTGTGG 
     1201 CACACCATTT TGACCTGGAC ACAGAGACAC CAGCCAACGG ATACCGTAGT TTGGTGCACA 
     1261 CAGCCCGATG CTGCCTGGCA CACCTACTAC ACAAATCCCG CTATGTGGCT TCTAACCGCA 
     1321 AAAGTATCTT CTTCCGTGCC AGCCACAACC TAGCAGAGCT GGAGGCCTAC CTGGCCGCCC 
     1381 TCACCCAGCT CCGTGCTATG GCCTACTATG CCCAGCGCCT GCTGACCATC AACCGACCAG 
     1441 GAGTGCTCTT CTTCGAGGGT GATGAAGGAC TCACCGCTGA CTTCCTGCAA GAGTATGTCA 
     1501 CGCTACACAA AGGCTGCTTC TACGGCCGCT GCCTGGGCTT CCAGTTCACA CCTGCCATCC 
     1561 GGCCGTTCCT GCAGACTCTC TCCATCGGGC TGGTGTCCTT CGGGGAGCAC TACAAACGCA 
     1621 ACGAGACAGG CCTCAGTGTG ACCGCCAGTT CCCTCTTTAC CGGTGGCCGA TTCGCCATAG 
     1681 ACCCAGAGTT GCGTGGGGCT GAATTTGAAC GCATCATACA GAACCTGGAT GTGCACTTCT 
     1741 GGAAAGCCTT CTGGAACATC ACTGAGATTG AGGTGCTGTC GTCTCTGGCC AACATGGCAT 
     1801 CAACCACTGT GAGGGTAAGC CGCCTGCTCA GCTTGCCACC TGAGGCCTTT GAGATGCCAC 
     1861 TCACCTCTGA TCCCAGGCTC ACAGTTACCA TCTCACCTCC CTTGGCACAC ACGGGACCAG 
     1921 CTCCTGTGCT AGCCAGGCTC ATCTCCTATG ACCTACGGGA AGGACAGGAC AGCAAGGTAC 
     1981 TCAACAGCCT GGCAAAATCT GAGGGCCCAC GCCTGGAGCT GCGCCCACGG CCTCACCAAG 
     2041 CACCCCGTTC ACGGGCCCTG GTTGTTCACA TCCACGGAGG CGGCTTTGTG GCACAGACCT 
     2101 CTAAATCCCA CGAGCCCTAC CTCAAGAACT GGGCCCAGGA GCTAGGAGTC CCTATCTTCT 
     2161 CCATCGACTA CTCCCTGGCC CCCGAGGCTC CCTTTCCCCG AGCGCTGGAG GAGTGTTTTT 
     2221 TTGCCTACTG CTGGGCTGTC AAGCACTGTG ACCTGCTTGG TTCAACTGGA GAGCGGATAT 
     2281 GCCTTGCAGG GGACAGTGCA GGTGGGAATC TCTGCATCAC TGTGTCCCTT CGGGCAGCAG 
     2341 CCTATGGAGT GAGGGTGCCA GATGGCATCA TGGCAGCCTA CCCAGTTACC ACCCTGCAGT 
     2401 CCTCTGCTTC TCCCTCTCGT CTGCTGAGCC TCATGGACCC TCTTCTACCA CTGAGCGTAC 
     2461 TCTCTAAGTG TGTCAGTGCC TATTCAGGGA CAGAGGCAGA GGACCATTTT GACTCAGACC 
     2521 AGAAGGCACT AGGCGTGATG GGGCTGGTGC AGAGAGACAC TTCGCTGTTC CTCAGAGACC 
     2581 TCCGACTGGG TGCCTCCTCA TGGCTCAACT CCTTCCTGGA ACTAAGTGGA CGCAAGCCCC 
     2641 AAAAGACCAC ATCGCCCACA GCAGAGTCTG TGCGCCCCAC GGAGTCTATG CGCAGGAGTG 
     2701 TGTCTGAGGC AGCCCTGGCC CAGCCTGAGG GCTTACTGGG CACAGATACC TTGAAGAAGC 
     2761 TGACAATAAA GGACTTGAGC AACTCAGAGC CTTCAGACAG CCCCGAGATG TCACAGTCAA 
     2821 TGGAGACACT TGGCCCCTCC ACACCCTCTG ATGTCAACTT TTTTCTGCGG CCTGGGAATT 
     2881 CCCAGGAAGA GGCTGAAGCC AAAGATGAAG TGAGACCCAT GGACGGAGTC CCCCGCGTGC 
     2941 GCGCTGCTTT CCCTGAGGGG TTTCACCCCC GGCGCTCAAG CCAAGGTGTC CTCCACATGC 
     3001 CCCTCTACAC GTCACCCATA GTCAAGAACC CCTTCATGTC TCCTCTGCTG GCCCCTGACA 
     3061 GCATGCTGAA GACCTTGCCG CCTGTGCACC TTGTGGCTTG CGCTCTGGAC CCCATGCTAG 
     3121 ATGACTCGGT CATGTTCGCG CGGCGACTGC GCGACCTGGG CCAGCCGGTG ACGCTGAAAG 
     3181 TGGTAGAAGA TCTGCCGCAT GGCTTCCTGA GCCTGGCGGC ACTGTGTCGC GAGACCCGGC 
     3241 AGGCCACGGA GTTCTGCGTG CAGCGCATCC GGCTGATCCT CACCCCGCCT GCTGCACCAC 
     3301 TGAACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3361 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3421 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3481 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3541 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3601 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3661 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3721 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3781 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3841 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3901 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3961 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4021 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4081 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4141 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4201 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4261 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4321 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4381 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4441 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4501 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4561 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4621 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4681 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4741 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4801 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4861 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4921 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4981 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5041 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5101 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5161 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5221 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5281 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5341 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5401 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5461 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5521 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5581 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5641 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5701 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5761 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5821 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5881 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5941 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6001 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6061 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6121 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6181 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6241 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6301 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6361 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6481 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6541 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6601 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6661 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6721 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6781 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6841 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6901 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6961 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7021 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7081 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7141 AAATTTAACG CGAATTTT
//