LOCUS       MR210344                7077 bp    DNA     circular  SYN 
DEFINITION  Bop1 (Myc-DDK-tagged) - Mouse block of proliferation 1 (Bop1)
ACCESSION   NM_013481
VERSION     NM_013481.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3234..3325 
                     /label="Myc-DDK"  
     misc_feature    3355..3940 
                     /label="PolyA signal"  
     misc_feature    3412..3431 
                     /label="Restriction Enzyme"  
     misc_feature    4216..4888 
                     /label="ColE1"  
     misc_feature    5318..6112 
                     /label="Kan/Neo"  
     misc_feature    6147..6449 
                     /label="SV40 ori"  
     misc_feature    6535..7012 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3216..3222 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3216 
                     /label="Bop1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGGCG TGTGGTAAGC CCCACATGTC ACCGGCATCG CTGCCGGGGA 
     1081 AACGACGTTT GGAACCTGAT CAGGAGCTGC AGATACAAGA GCCTCCTCTC CTCAGCGATC 
     1141 CTGACTCCAG TCTCTCTGAC AGCGAGGAGA GTGTGTTTTC AGGCCTCGAA GATTTGGGCA 
     1201 GTGACTCGAG TGAGGAAGAC ACTGAAGGAG TGGCCGGATC CAGCGGTGAC GAAGACAACC 
     1261 ATAGGGCAGA GGAGACCTCT GAGGAGCTGG CACAGGCTGC CCCTCTTTGC TCAAGGACAG 
     1321 AGGAAGCAGG TGCTCTGGCC CAAGATGAAT ATGAAGAGGA CAGCTCTGAT GAGGAGGACA 
     1381 TTCGGAACAC TGTGGGCAAT GTGCCCCTGG CATGGTACGA TGAATTCCCA CATGTGGGTT 
     1441 ATGACCTGGA TGGCAAACGT ATCTACAAGC CCCTGCGGAC ACGAGATGAG CTCGACCAGT 
     1501 TTCTGGACAA AATGGATGAC CCAGATTTCT GGCGCACTGT GCAAGACAAG ATGACAGGGC 
     1561 GTGATCTGCG GCTAACTGAT GAGCAGGTTG CCCTGGTACA CCGGCTTCAG AGAGGCCAGT 
     1621 TCGGAGATTC AGGCTTCAAT CCCTATGAGC CAGCTGTGGA CTTCTTCAGC GGTGACATCA 
     1681 TGATCCACCC TGTGACCAAC CGCCCAGCTG ACAAGCGTAG TTTCATCCCA TCCCTAGTTG 
     1741 AAAAGGAGAA GGTGTCTCGA ATGGTACATG CCATCAAGAT GGGGTGGATC AAGCCTCGAC 
     1801 GGCCCCACGA CCCCACCCCT AGCTTCTATG ACCTGTGGGC CCAGGAGGAT CCGAATGCTG 
     1861 TGTTGGGACG CCACAAGATG CATGTGCCTG CCCCCAAGCT GGCCTTGCCT GGCCATGCCG 
     1921 AGTCTTACAA CCCACCTCCC GAGTACCTGC CCACTGAGGA GGAGCGCTCG GCGTGGATGC 
     1981 AGCAGGAGCC TGTGGAGAGG AAGCTTAACT TCTTGCCACA GAAATTCCCC AGCTTGAGGA 
     2041 CAGTGCCTGC TTACAGCCGC TTCATCCAGG AGCGTTTCGA GCGCTGCCTC GATTTGTATC 
     2101 TGTGCCCACG GCAACGCAAG ATGAGGGTGA ATGTGGACCC CGAAGACCTC ATCCCCAAAC 
     2161 TCCCTCGGCC AAGAGACCTT CAGCCTTTCC CTGTGTGCCA GGCCCTCGTC TACAGGGGCC 
     2221 ACAGCGACCT TGTCCGTTGC CTCAGTGTCT CGCCAGGGGG CCAGTGGCTA GCTTCAGGTT 
     2281 CAGACGATGG CACGTTGAAG CTCTGGGAGG TGGCCACTGC CCGCTGTATG AAGACTGTAC 
     2341 ATGTTGGAGG TGTGGTGCGG AGCATCGCCT GGAACCCCAA TCCTACCATA TGCCTGGTAG 
     2401 CTGCTGCCAT GGATGATGCC GTGTTGCTGC TGAACCCAGC CCTGGGGGAC CGGCTGCTGG 
     2461 TGGGCAGCAC AGACCAGCTG CTGGAAGCCT TCACTCCACC TGAGGAGCCA GCTCTGCAGC 
     2521 CTGCCCGGTG GCTAGAGGTC TCGGAGGAAG AGCACCAGAG GGGTCTGCGC CTACGCATCT 
     2581 GCCACAGCAA ACCAGTGACA CAGGTGACCT GGCATGGGCG AGGGGACTAT TTGGCGGTGG 
     2641 TGCTGTCTAG TCAAGAGCAC ACGCAAGTGC TGCTTCACCA GGTGAGCAGG AGGCGCAGCC 
     2701 AGAGCCCGTT CCGCCGCAGC CACGGGCAGG TGCAGTGTGT GGCCTTCCAT CCCTCCCGGC 
     2761 CCTTCCTGCT CGTGGCCTCC CAGCGCAGCA TCCGCATTTA CCACCTGCTG CGCCAGGAGC 
     2821 TAACCAAGAA GCTGATGCCC AACTGCAAGT GGGTGTCCAG CATGGCTGTA CATCCAGCAG 
     2881 GTGACAACAT CATCTGTGGC AGCTATGACA GCAAACTGGT GTGGTTTGAC CTGGATCTTT 
     2941 CCACCAAGCC ATACAAAGTG CTGAGGCACC ACAAGAAGGC CTTGCGGGCT GTGGCCTTCC 
     3001 ACCCCCGATA CCCACTCTTT GCATCCGGCT CAGACGACGG CAGTGTTATC GTGTGCCATG 
     3061 GCATGGTGTA CAATGACCTG CTGCAGAACC CATTGCTGGT GCCTGTCAAG GTGCTGAAAG 
     3121 GACACACCCT GACCCGAGAT CTGGGTGTTC TGGATGTGGC CTTCCACCCC ACACAGCCGT 
     3181 GGGTCTTCTC CTCCGGGGCA GATGGCACCA TTCGACTCTT CAGCACGCGT ACGCGGCCGC 
     3241 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3301 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3361 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3421 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3481 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3541 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3601 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3661 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3721 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3781 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3841 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3901 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3961 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4021 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4081 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4141 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4201 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4261 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4321 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4381 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4441 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4501 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4561 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4621 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4681 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4741 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4801 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4861 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4921 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4981 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5041 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5101 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5161 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5221 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5281 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5341 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5401 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5461 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5521 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5581 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5641 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5701 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5761 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5821 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5881 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5941 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6001 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6061 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6121 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6181 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6241 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6301 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6361 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6421 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6481 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6541 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6601 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6661 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6721 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6781 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6841 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6901 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6961 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7021 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//