LOCUS       MR210326                7065 bp    DNA     circular  SYN 
DEFINITION  Rhobtb2 (Myc-DDK-tagged) - Mouse Rho-related BTB domain containing 2 (Rhobtb2)
ACCESSION   NM_153514
VERSION     NM_153514.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3222..3313 
                     /label="Myc-DDK"  
     misc_feature    3343..3928 
                     /label="PolyA signal"  
     misc_feature    3400..3419 
                     /label="Restriction Enzyme"  
     misc_feature    4204..4876 
                     /label="ColE1"  
     misc_feature    5306..6100 
                     /label="Kan/Neo"  
     misc_feature    6135..6437 
                     /label="SV40 ori"  
     misc_feature    6523..7000 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3204..3210 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3204 
                     /label="Rhobtb2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTCTGAC ATGGATTATG AAAGGCCAAA CGTAGAGACC ATCAAGTGCG 
     1081 TGGTGGTGGG GGACAACGCC GTGGGCAAGA CTAGGCTCAT CTGTGCCCGG GCCTGCAATG 
     1141 CCACCCTCAC CCAGTACCAG TTGCTTGCCA CCCATGTGCC CACAGTGTGG GCCATTGACC 
     1201 AGTATCGTGT GTGCCAGGAG GTGCTAGAAC GCTCCCGAGA TGTGGTAGAT GACGTCAGCG 
     1261 TCTCCCTGCG CCTCTGGGAC ACCTTCGGAG ACCACCACAA AGACCGGCGT TTTGCTTATG 
     1321 GAAGATCCGA CGTGGTGGTT TTGTGTTTCT CCATCGCCAA CCCCAATTCC CTCCACCATG 
     1381 TCAAGACCAT GTGGTACCCA GAAATCAAGC ATTTCTGCCC CAGAGCTCCT GTCATTCTGG 
     1441 TGGGCTGCCA GCTGGACCTG CGCTATGCTG ACCTGGAAGC CGTTAACAGG GCCAGGAGAC 
     1501 CTCTGGCTAG GCCCATCAAG CCCAATGAAA TCCTCCCCCC AGAGAAGGGT CGGGAAGTCG 
     1561 CCAAAGAGCT GGGGATCCCT TACTACGAGA CCAGTGTGGT GGCCCAGTTT GGTATCAAGG 
     1621 ACGTCTTTGA CAATGCCATT CGGGCTGCGC TCATCTCCCG CCGCCACCTG CAGTTCTGGA 
     1681 AATCACATCT GCGCAATGTG CAGCGGCCTC TACTGCAAGC ACCCTTCCTC CCCCCCAAGC 
     1741 CACCGCCCCC CATCATCGTG GTGCCCGATC CCCCCTCCAG CAGCGAGGAG TGCCCCGCCC 
     1801 ACCTCCTGGA AGACCCGCTC TGTGCGGATG TCATCCTGGT GCTGCAGGAG CGCGTGCGCA 
     1861 TCTTTGCCCA CAAAATCTAC CTCTCCACCT CCTCCTCCAA ATTCTATGAC TTATTCCTCA 
     1921 TGGACCTGAG TGAGGGGGAG CTGGGGGGCC CCTCAGGGTC AGGGGGGCCC CGCCCAGAGG 
     1981 ACCACCGGAG CCACCCTGAA CAACACCACC ATCATCACCA TCACCACCAT GGGCGGGACT 
     2041 TCCTGCTTCG GGCAGCCAGC TTTGACGTAT GTGAGAGTGT GGATGAGGCC GGCGGCTCTG 
     2101 GTCCGGCTGG CCTAAGGGCT TCAACCAGTG ACGGGATCTT ACGGGGTAAT GGAACAGGGT 
     2161 ACCTGCCAGG CAGAGGACGT GTGCTGTCTT CCTGGAGCCG AGCTTTTGTG AGTATCCAGG 
     2221 AAGAGATGGC AGAGGATCCT CTGACCTTCA AATCCCGGCT GATGGTGGTG GTGAAGATGG 
     2281 ATAACTCCAT TCAGCCGGGG CCCTTCCGGG CTGTTCTCAA GTACCTGTAC ACAGGGGAGC 
     2341 TAGGTGAGAA TGAGCGGGAC CTCATGCACA TTGCTCACAT TGCCGAGCTG CTAGAGGTCT 
     2401 TTGATCTACG AATGATGGTG GCCAACATTC TCAACAACGA AGCCTTCATG AATCAGGAGA 
     2461 TCACCAAGGC CTTCCATGTA CGCCGGACCA ACCGGGTTAA GGAGTGTTTG GCAAAGGGCA 
     2521 CCTTTTCAGA TGTGACCTTC ATCCTGGATG ATGGGACCAT CAGCGCCCAT AAGCCCCTGC 
     2581 TGATTTCCAG CTGTGACTGG ATGGCTGCCA TGTTTGGGGG GCCATTTGTG GAGAGTTCTA 
     2641 CCAGGGAGGT GGTGTTTCCA TACACAAGCA AGAGCTGCAT GAGAGCTGTG TTGGAGTACC 
     2701 TCTATACAGG CATGTTCACC TCCAGCCCAG ACCTGGATGA TATGAAGCTC ATTGTCTTGG 
     2761 CTAACCGCCT CTGCCTGCCG CACTTGGTTG CCCTCACAGA GCAGTACACA GTGACTGGAC 
     2821 TGATGGAGGC GACCCAGATG ATGGTGGACA TCGATGGGGA TGTCCTTGTA TTCTTGGAAT 
     2881 TGGCACAGTT CCACTGCGCG TACCAGTTGG CCGACTGGTG TCTTCACCAT ATCTGCACCA 
     2941 ACTACAACAA CGTGTGCCGC AAGTTCCCCA GAGACATGAA GGCTATGTCT CCAGAAAACC 
     3001 AGGAGTACTT TGAGAAACAC CGGTGGCCGC CAGTCTGGTA CCTGAAAGAA GAGGACCACT 
     3061 ATCAGCGTGC GCGGAAGGAG CGAGAGAAAG AGGACTATTT ACACCTGCGG AGGCAGCCCA 
     3121 AGCGGCGGTG GCTGTTTTGG AACAGTCCCT CATCACCGTC CTCCTCAGCA GCGGGCTCAG 
     3181 CATCCCCCTC CTCCTCCTCC TCGGCTGTGG TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3241 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3301 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3361 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3421 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3481 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3541 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3601 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3661 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3721 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3781 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3841 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3901 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3961 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4021 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4081 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4141 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4201 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4261 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4321 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4381 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4441 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4501 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4561 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4621 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4681 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4741 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4801 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4861 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4921 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4981 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5041 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5101 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5161 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5221 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5281 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5341 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5401 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5461 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5521 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5581 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5641 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5701 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5761 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5821 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5881 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5941 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6001 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6061 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6121 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6181 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6241 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6301 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6361 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6421 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6481 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6541 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6601 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6661 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6721 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6781 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6841 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6901 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6961 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7021 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//