LOCUS       MR210307                7056 bp    DNA     circular  SYN 
DEFINITION  Nadsyn1 (Myc-DDK-tagged) - Mouse NAD synthetase 1 (Nadsyn1)
ACCESSION   NM_030221
VERSION     NM_030221.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3213..3304 
                     /label="Myc-DDK"  
     misc_feature    3334..3919 
                     /label="PolyA signal"  
     misc_feature    3391..3410 
                     /label="Restriction Enzyme"  
     misc_feature    4195..4867 
                     /label="ColE1"  
     misc_feature    5297..6091 
                     /label="Kan/Neo"  
     misc_feature    6126..6428 
                     /label="SV40 ori"  
     misc_feature    6514..6991 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3195..3201 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3195 
                     /label="Nadsyn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCGGAAA GTGACCGTGG CCACCTGTGC ACTCAATCAG TGGGCCCTGG 
     1081 ACTTTGAGGG CAATTTCCAG AGGATTTTAA AGAGTATTCA AATTGCCAAA GGCAAAGGTG 
     1141 CAAGATACAG GCTTGGACCA GAACTAGAAA TATGCGGCTA TGGATGTTGG GATCATTATC 
     1201 ACGAATCAGA CACTCTCCTG CATTCGCTCC AAGTTCTGGC TGCTCTTCTG GACTCTCCGG 
     1261 TCACTCAGGA CATCATCTGT GATGTGGGGA TGCCTATAAT GCACCGGAAT GTTCGCTACA 
     1321 ACTGCAGAGT CATCTTTCTC AACAGGAAGA TCCTGCTCAT CAGACCCAAG ATGGCCTTGG 
     1381 CCAACGAAGG CAACTACCGG GAACTGCGCT GGTTTACCCC CTGGACCAGG AGCCGACAAA 
     1441 CTGAGGAATA TGTCCTCCCT CGGATGTTAC AGGACCTGAC AAAGCAGAAA ACTGTGCCCT 
     1501 TTGGAGATGT GGTACTGGCC ACCCAAGACA CCTGTGTCGG GAGTGAGATC TGTGAGGAGC 
     1561 TCTGGACACC ACGCAGCCCC CACATCGACA TGGGCCTGGA TGGTGTGGAG ATCATTACCA 
     1621 ATGCCTCGGG CAGCCACCAC GTGCTCCGCA AAGCCCATAC CAGAGTGGAT CTGGTAACCA 
     1681 TGGCCACCTC CAAGAATGGT GGCATCTACC TGCTGGCCAA CCAGAAGGGC TGTGATGGTG 
     1741 ACCGTCTCTA CTACGATGGC TGTGCCATGA TTGCTATGAA CGGGAGCATC TTTGCCCAGG 
     1801 GCACTCAGTT CTCATTGGAT GATGTGGAGG TTCTCACTGC CACCCTGGAC CTGGAGGACG 
     1861 TTAGGAGCTA CAAGGCAGAG ATCTCATCTC GGAACCTGGA GGCAACCAGG GTGAGCCCAT 
     1921 ATCCCCGGGT GACGGTGGAC TTTGCCCTTT CGGTCAGTGA AGACTTGTTG GAGCCAGTGT 
     1981 CAGAGCCTAT GGAATGGACA TACCACAGGC CTGAGGAGGA GATAAGCCTC GGCCCTGCCT 
     2041 GCTGGCTCTG GGACTTCCTT CGACGGAGCA AACAGGCCGG GTTTTTCCTT CCCCTGAGTG 
     2101 GTGGTGTGGA CAGTGCAGCC AGCGCCTGTA TCGTCTACTC CATGTGCTGC CTGGTCTGCG 
     2161 ATGCTGTGAA GAGCGGAAAT CAGCAAGTGC TGACAGACGT CCAGAACCTG GTGGACGAGA 
     2221 GCAGCTACAC CCCTCAGGAT CCCCGGGAGC TCTGCGGACG GCTGCTCACT ACTTGTTACA 
     2281 TGGCCAGCGA GAACTCCTCC CAGGAGACCC ACAGCAGAGC CACAAAACTG GCTCAGCTGA 
     2341 TTGGAAGCTA CCACATCAAT CTGAGCATCG ACACTGCTGT GAAGGCTGTC TTGGGCATCT 
     2401 TCAGCCTGAT GACCGGGAAG TTGCCTCGGT TCTCAGCCCA CGGAGGGAGC AGCAGGGAGA 
     2461 ACCTGGCCTT GCAGAATGTG CAGGCTCGGA TTAGGATGGT CCTTGCCTAC CTGTTTGCTC 
     2521 AGCTGAGCCT CTGGTCTCGG GGTGCTCGAG GAAGCCTTCT TGTGCTTGGA TCTGCCAATG 
     2581 TGGATGAGAG TCTCCTCGGC TACCTGACCA AGTACGACTG CTCCAGTGCG GACATCAACC 
     2641 CCATAGGCGG CATCAGCAAG ACGGACCTGA GAGCCTTTGT CCAGTTTTGC GCAGAGCGCT 
     2701 TCCAGCTTCC TGTTCTGCAG ACCATCCTGT CAGCACCGGC CACTGCAGAG CTGGAGCCCT 
     2761 TAGCTGATGG GCAGGTGTCT CAGATGGATG AGGAAGACAT GGGGATGACG TACGCAGAAC 
     2821 TCTCCATCTT CGGCAGGCTC CGGAAGGTCG CCAAGGCAGG CCCCTACAGC ATGTTCTGCA 
     2881 AACTGCTCAA CATGTGGAGA GACAGCTATA CACCAACACA GGTAGCTGAG AAGGTGAAGC 
     2941 TATTTTTCTC AAAGTACTCC ATGAACAGGC ACAAGATGAC AACCCTCACA CCAGCATATC 
     3001 ATGCTGAAAA CTACAGTCCT GACGACAACA GGTTTGACCT GAGACCCTTT CTGTATAACA 
     3061 CGAGATGGCC CTGGCAGTTC CTCTGCATTG ACAACCAGGT TCTACAGCTT GAGAGGAAAG 
     3121 CATCGCAGAC CCGGGAAGAA CAGGTCCTGG AGCACTTCAA AGAGCCATCA CCTATATGGA 
     3181 AACAGCTCCT CCCCAAGGAC CCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3241 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3301 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3361 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3421 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3481 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3541 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3601 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3661 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3721 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3781 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3841 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3901 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3961 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4021 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4081 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4141 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4201 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4261 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4321 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4381 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4441 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4501 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4561 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4621 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4681 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4741 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4801 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4861 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4921 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4981 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5041 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5101 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5161 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5221 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5281 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5341 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5401 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5461 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5521 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5581 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5641 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5701 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5761 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5821 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5881 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5941 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6001 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6061 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6121 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6181 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6241 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6301 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6481 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6541 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6601 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6661 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6721 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6781 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6841 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6901 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6961 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7021 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//