LOCUS       MR210279                7044 bp    DNA     circular  SYN 
DEFINITION  Bbs2 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2)
ACCESSION   NM_026116
VERSION     NM_026116.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3201..3292 
                     /label="Myc-DDK"  
     misc_feature    3322..3907 
                     /label="PolyA signal"  
     misc_feature    3379..3398 
                     /label="Restriction Enzyme"  
     misc_feature    4183..4855 
                     /label="ColE1"  
     misc_feature    5285..6079 
                     /label="Kan/Neo"  
     misc_feature    6114..6416 
                     /label="SV40 ori"  
     misc_feature    6502..6979 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3183..3189 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3183 
                     /label="Bbs2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCTGCCT GTGTTCACTC TGAAACTGCG CCACAAAATC AGCCCCCGCA 
     1081 TGGTGGCCAT AGGGCGCTAC GACGGGACTC ACCCGTGCTT GGCTGCCGCC ACCCAAGCGG 
     1141 GCAAGGTTTT CATTCATAAC CCTCACACGC GGAGCCAGCA TTTCAGTGCT TCCAGAGTGT 
     1201 TCCAGAGCCC TCTGGAGTCT GATGTCTCTC TGCTCAACAT TAACCAGACG GTCAGCTGCC 
     1261 TGGGTTCGGG AGTCTTGAAC CCTGAGCTTG GCTATGATAC TCTTTTAGTG GGGACACAGA 
     1321 CCAGTCTTTT GGCTTACGAC ATCTACAATA ATTCAGATTT GTTCTACAGA GAGGTCTCAG 
     1381 ATGGGGCAAA TGCCATTGTG CTGGGGACCC TGGGAGACAT TGCCCCACCT CTTGCAATCA 
     1441 TCGGTGGAAA CTGTGCTCTG CAGGGTTTCG ATCACGAAGG AAATGATCTC TTTTGGACGG 
     1501 TTACTGGAGA CAATGTGCAT TCCTTGGCTC TGTGTGACTT TGACGGTGAT GGGAAGACAG 
     1561 AGCTTCTTGT TGGATCTGAA GATTTTGACA TTCGAGTTTT TAAAGAAGAT GAGATTGTGG 
     1621 CAGAAATGAC AGAGACAGAG ATAGTCACCT CTCTGTGTCC TATGTATGGC AGTCGGTTTG 
     1681 GTTACGCCCT TTCTAATGGC ACAGTTGGAG TTTATGACAA AACAGCCCGG TACTGGAGAA 
     1741 TTAAATCCAA AAACCATGCC ATGAGCATCC ATGCTTTTGA CATTAATTCT GATGGAGTGT 
     1801 GTGAACTGAT AACCGGGTGG TCTAATGGGA AGGTTGATGC TCGCAGTGAC CGGACTGGAG 
     1861 AGGTCATATT TAAAGACAAC TTCTCTTCTG CTGTTGCTGG TGTGGTAGAG GGAGATTACC 
     1921 GGATGGATGG CCACGTACAG TTAATCTGCT GCTCAGTGGA TGGGGAAATC CGAGGCTACC 
     1981 TGCCAGGCAC AGCTGAGATG AAGGGGAACC TCCTGGACAC AAGTGTGGAG CAGGACCTGA 
     2041 TCCGAGAGCT GAGTCAGAAA AAGCAGAACC TGTTGCTAGA ACTTCGCAAC TATGAGGAGA 
     2101 GCACCAAGGC AGAACTGAGC AGTCCCCTGA ACGAGGCCGA TGGGCAGAAA GGCATAATCC 
     2161 CAGCCAATAC CAGGCTCCAC ACAGCGCTCT CAGTCAATAT GGGGAATGAC TTACAAGATG 
     2221 CGCATGCGGA ATTAGGCATT TCCACTTCCA ATGATACTAT CATCCGAGCA GTACTAATTT 
     2281 TTGCAGAGGG AATTTTTGTG GGTGAAAGCC ATGTGGTCCA CCCCAGCATT CACAACCTTT 
     2341 CCAGCTCCCT CCGGGTACCA ATTACACCTC CCAAAGATGT CCCTGTGGAT CTGCACCTGA 
     2401 AAACCTTCGT GGGTTACAGA AGCAGCACCC AGTTCCATGT GTTTGAACTG ACAAGACAGC 
     2461 TGCCCCGATT CACCATGTAT GCATTGACAA GCCCAGATGC AGCTAGCGAG CCTGTCAGCT 
     2521 ACGTGAACTT CAGTGTTGCC GAGCGCACAC AGAGGATGGT CACGTGGCTC AACCAGAACT 
     2581 TTCTGTTGCC AGAAGACAGT AACGTTCAGA ATTCCCCGTT TCACGTCTGT TTCACATCGT 
     2641 TACGCAATGG TGGCCAGCTC TACATAAAAA TGAAACAAAG TGGTGAGATC ACTGTGAATA 
     2701 CTGACGATAT TGATCTAGCT GGTGACATCA TCCAGTCGAT CGCGTCCTTT TTTGCTATTG 
     2761 AAGACCTTCA GGTAGAAGCA GATTTCCCTG TGTACTTCGA GGAACTACGG AAAGTGCTGG 
     2821 TGAAGGTGGA TGAGTATCAT TCAGTGCATC AAAAGCTCAG CGCCGACATG GCTGATAATT 
     2881 CTAACCTCAT CCGAAGTTTG CTGGTCAGAG CGGAGGATGC TCGGCTGATG AGGGACATGA 
     2941 AAACAATGAA GAGTCGTTAC ATGGAGCTCT ATGACCTTAA TAAAGATTTG CTGAATGGAT 
     3001 ACAAGATTCG CTGTAACAAC CACACAGAAC TGCTAGGAAA CCTGAAGGCG GTGAACCAAG 
     3061 CAATCCAAAG AGCAGGCCGT CTGCGCGTTG GAAAGCCAAA GAACCAGGTG ATCAGTGCTT 
     3121 GTCGGGATGC GATTCGAAGC AACAACATCA ACACCCTCTT CAGAATCATG CGAGTGGGCA 
     3181 CTGCTCCTTC AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3241 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3301 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3361 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3421 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3481 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3541 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3601 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3661 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3721 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3781 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3841 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3901 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3961 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4021 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4081 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4141 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4201 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4261 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4321 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4381 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4441 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4501 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4561 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4621 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4681 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4741 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4801 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4861 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4921 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4981 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5041 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5101 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5161 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5221 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5281 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5341 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5401 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5461 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5521 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5581 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5641 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5701 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5761 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5821 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5881 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5941 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6001 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6061 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6121 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6181 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6241 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6301 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6361 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6421 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6481 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6541 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6601 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6661 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6721 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6781 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6841 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6901 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6961 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7021 TAACAAAAAT TTAACGCGAA TTTT
//