LOCUS       MR210149                6993 bp    DNA     circular  SYN 
DEFINITION  Shkbp1 (Myc-DDK-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1)
ACCESSION   NM_138676
VERSION     NM_138676.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3150..3241 
                     /label="Myc-DDK"  
     misc_feature    3271..3856 
                     /label="PolyA signal"  
     misc_feature    3328..3347 
                     /label="Restriction Enzyme"  
     misc_feature    4132..4804 
                     /label="ColE1"  
     misc_feature    5234..6028 
                     /label="Kan/Neo"  
     misc_feature    6063..6365 
                     /label="SV40 ori"  
     misc_feature    6451..6928 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3132..3138 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3132 
                     /label="Shkbp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGTCGCG ACGACTGCAG TGGAGGGGGT CCCGAGTCGG GGAGCACCCG 
     1081 GGGAAGTGAT TCATCTGAAC GTAGGGGGCA AGCGGTTCAG TACCTCTCGC CAGACGCTTA 
     1141 CCTGGATCCC AGACTCCTTC TTTTCCAGTC TTTTGAGCGG ACGCATCTCA ACCCTGAAAG 
     1201 ATGAGACCGG AGCTATCTTC ATCGACCGGG ACCCCACCGT CTTTGCCCCC ATCCTTAATT 
     1261 TCCTGCGGAC CAAAGAGTTA GATCCCAGGG GTGTCCATGG TTCCAGCCTC CTCCATGAAG 
     1321 CCCAATTCTA CGGACTCACT CCACTGGTTC GTCGCCTGCA GGTGAGGGAA GAGTTGGATC 
     1381 GATCTTCCTG CGGAAATGTC CTCTTCAATG GTTATCTACC TCCTCCAGTG TTCCCAGTGA 
     1441 AGCGGCGAAA CCGGCACAGC CTGGTGGGGC CCCAGCAAAT AGGGGGACGC CCAGCCCCTG 
     1501 TTCGACGGAG CAACACGATG CCCCCCAACT TGGGTAATGC AGGGCTGCTG GGCCGAATGT 
     1561 TAGATGAGAG AGCACCTCCA TCCCCATCAG GCCAACCAGA GGAGCCAGGG ATGGTGCGCC 
     1621 TGGTGTGTGG TCACCACAAC TGGATTGCTG TGGCATATAC CCACTTTCTC GTCTGTTATA 
     1681 GGTTGAAGGA AGCCTCGGGC TGGCAGCTTG CATTCTCCAG CCCGCGCCTG GACTGGCCCA 
     1741 TCGAGCGCCT GGCTCTTGCG GCTCGGGTGC TTGGCGGTGC TCCGGGTGAG CATGACAAGA 
     1801 TGGTGGCTGC AGCTACTGGC AGCGAGATCC TTCTGTGGGC CCTACAAGCA CAAGGTGGTG 
     1861 GCTCTGAGAT AGGTGTCTTC CACCTGGGTG TGCCAGTGGA GGCCTTGTTC TTTGTTGGGA 
     1921 ATCAGCTCAT TGCCACAAGT CACACAGGGC GCATCGGTGT CTGGAACGCT GTGACCAAGC 
     1981 ACTGGCAGGT CCAGGAGGTC CAGCCTATCA CGAGCTATGA TGCAGCCGGC TCCTTCCTCC 
     2041 TCCTGGGCTG CAGCAATGGC TCCATCTATT ATGTAGATGT GCAGAAATTC CCCCTGCGGA 
     2101 TGAAGGACAA CGATCTCCTT GTCAGTGAGC TCTACCGAGA CCCAGCAGAG GATGGGGTCA 
     2161 CCGCCCTCAG CGTCTACCTC ACCCCCAAGA CCAGTGACAG TGGGAACTGG ATTGAGATTG 
     2221 CCTATGGCAC CAGCTCCGGG GTGGTGCGGG TCATTGTGCA GCACCCTGAG ACGGTGGGCT 
     2281 CGGGACCCCA GCTCTTCCAG ACCTTCAGTG TGCACCGCAG CCCGGTCACT AAGATCATGC 
     2341 TGTCAGAGAA GCATCTTATC TCTGTGTGTG CCGACAACAA CCATGTTCGG ACGTGGTCTG 
     2401 TGACCCGCTT TCGAGGCATG ATCTCCACCC AGCCTGGTTC CACTCCGCTG GCATCCTTCA 
     2461 AGATCCTGGC GCTGGAATCT GTTGATGGGC TCGGTGGCTG CAGTGCTGGC AATGACATTG 
     2521 GTCCCTATGG TGAACGGGAT GACCAGCAGG TGTTCATCCA GAAAGTGGTG CCAAATGCCA 
     2581 GTCAGCTATT TGTGCGTCTC TCTTCCACTG GGCAGCGGGT GTGTTCTGTG CGCTCGGTGG 
     2641 ATGGGTCTCC CACGACTGCC TTCACAGTGT TGGAGTGTGA GGGCTCCAGG CGGCTGGGCT 
     2701 CCAGACCCCG ACGTTACCTG CTCACTGGTC AGGCCAACGG CAGCCTGGCC ATGTGGGACC 
     2761 TGACCACTGC CATGGATGGC CTGGGCCAAA CTCCTGCAGG CGGCCTGACA GAAGAAGAGC 
     2821 TGATGGACCA ACTGGAGCAA TGTGAGCTGA GCCCATTGGC TTCCTCTCGG GGCTCTTTCC 
     2881 CCAGCCCCTC ACCCAGGACC TCTCTTACCA GTCTCCACTC AGCCTCCAGC AACACCTCCC 
     2941 TGTATGGCCC CAGAGGGAGC CCCAGCCCAC CACAGGCAGA GGCCCGTCGC CGCGGGGCAG 
     3001 GAAGCTTCGT GGATCGGTGT CAGGAACTGG CAAGGGGTGC GCCAGAGCTC AGATGGCCGC 
     3061 CAACACCAGC CCCTCGACCC TCCACCAGTC TTGGGAATCC CCTGACAGCT CTCAAGAAAA 
     3121 CTCTTAACGA AACTTCCTTT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3181 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3241 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3301 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3361 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3421 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3481 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3541 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3601 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3661 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3721 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3781 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3841 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3901 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3961 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4021 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4801 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4861 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4921 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4981 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5041 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5101 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5161 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5221 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5281 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5341 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5401 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5461 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5521 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5581 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5641 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5701 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5761 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5821 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5881 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5941 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6001 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6061 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6121 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6181 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6241 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6301 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6361 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6421 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6481 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6541 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6601 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6661 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6721 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6781 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6841 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6901 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6961 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//