LOCUS       MR210127                6981 bp    DNA     circular  SYN 
DEFINITION  Capn2 (Myc-DDK-tagged) - Mouse calpain 2 (Capn2)
ACCESSION   NM_009794
VERSION     NM_009794.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3138..3229 
                     /label="Myc-DDK"  
     misc_feature    3259..3844 
                     /label="PolyA signal"  
     misc_feature    3316..3335 
                     /label="Restriction Enzyme"  
     misc_feature    4120..4792 
                     /label="ColE1"  
     misc_feature    5222..6016 
                     /label="Kan/Neo"  
     misc_feature    6051..6353 
                     /label="SV40 ori"  
     misc_feature    6439..6916 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3120..3126 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3120 
                     /label="Capn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGCATC GCGATAAAGC TGGCCAAGGA CCGCGAAGCG GCCGAGGGGC 
     1081 TGGGGTCGCA TGAGAGAGCC ATCAAGTACC TCAACCAGGA CTACGAGACG CTGCGGAACG 
     1141 AGTGCCTGGA GGCCGGGGCG CTCTTCCAGG ATCCTTCCTT CCCCGCCCTG CCGTCATCCT 
     1201 TGGGCTATAA GGAGTTGGGG CCCTACTCCA GCAAAACTCG GGGCATCGAA TGGAAGCGGC 
     1261 CCACGGAGAT CTGCGCTGAC CCCCAGTTCA TTATTGGAGG AGCCACCCGC ACAGACATCT 
     1321 GCCAAGGAGC CCTGGGGGAC TGCTGGCTGC TGGCTGCCAT CGCCTCCCTC ACCTTGAATG 
     1381 AGGAAATCCT GGCTCGGGTT GTGCCTCCAG ATCAGAGCTT CCAGGAAAAC TATGCAGGCA 
     1441 TCTTCCACTT CCAGTTCTGG CAATATGGCG AGTGGGTGGA GGTGGTGGTG GACGACAGGC 
     1501 TGCCCACTAA GGACGGGGAG CTGCTCTTTG TGCATTCAGC TGAAGGGAGT GAGTTCTGGA 
     1561 GTGCCCTTCT GGAGAAAGCC TATGCCAAGA TCAATGGGTG CTATGAAGCA CTCTCAGGGG 
     1621 GTGCCACCAC GGAAGGCTTT GAAGACTTCA CAGGAGGCAT TGCTGAGTGG TATGAGCTGA 
     1681 GGAAGCCTCC TCCCAACTTG TTCAAGATCA TCCAGAAGGC TTTGGAGAAA GGTTCTCTGC 
     1741 TTGGCTGCTC TATCGATATC ACCAGCGCTG CAGATTCTGA GGCCGTTACG TACCAGAAGT 
     1801 TGGTGAAAGG ACATGCGTAC TCTGTCACCG GAGCCGAGGA GGTTGAAAGT TCAGGAAGCC 
     1861 TGCAGAAATT GATCCGCATC AGGAATCCCT GGGGACAAGT AGAGTGGACC GGGAAGTGGA 
     1921 ATGACAATTG CCCCAGCTGG AACACGGTTG ACCCAGAAGT AAGGGCAAAT CTAACCGAAC 
     1981 GGCAGGAGGA CGGAGAATTC TGGATGTCCT TCAGTGACTT CCTGAGACAC TACTCTCGCC 
     2041 TGGAGATCTG CAACCTGACC CCGGACACCC TCACCTGTGA CTCCTATAAG AAGTGGAAAC 
     2101 TCACCAAGAT GGATGGGAAC TGGAGGCGAG GCTCCACCGC AGGGGGCTGC AGGAATTACC 
     2161 CAAATACCTT CTGGATGAAC CCTCAGTACC TAATTAAGCT GGAGGAAGAA GACGAAGATG 
     2221 AGGAGGATGG GGAGAGAGGC TGTACCTTCC TGGTGGGTCT CATCCAGAAG CACCGGCGAC 
     2281 GGCAGAGGAA GATGGGCGAG GACATGCACA CCATTGGCTT CGGCATCTAT GAGGTTCCAG 
     2341 AGGAGCTAAC AGGGCAGACC AACATCCACC TCGGCAAAAA CTTTTTCCTC ACAACCCGAG 
     2401 CCAGGGAGCG GTCAGATACC TTCATTAACC TCCGCGAGGT CCTCAACCGC TTCAAGCTGC 
     2461 CCCCGGGAGA GTATGTCCTC GTTCCCTCCA CCTTCGAACC CCACAAGGAT GGCGATTTCT 
     2521 GCATCCGAGT CTTCTCGGAG AAGAAGGCTG ACTACCAAGC TGTTGATGAC GAGATCGAGG 
     2581 CCAACATTGA AGAGATTGAC GCCAATGAGG AGGACATTGA CGATGGATTC CGAAGGCTCT 
     2641 TTGTTCAGCT GGCTGGAGAG GATGCAGAGA TCTCTGCCTT TGAGCTGCAG ACCATCTTAA 
     2701 GAAGAGTTCT AGCCAAACGC CAAGACATCA AGTCAGACGG CTTCAGCATC GAGACCTGTA 
     2761 AGATCATGGT GGACATGCTG GATGAAGATG GGAGTGGCAA GCTTGGCCTG AAGGAGTTCT 
     2821 ACATCCTCTG GACGAAGATT CAGAAATACC AAAAAATCTA CCGGGAAATC GATGTGGACA 
     2881 GGTCTGGAAC CATGAATTCC TACGAGATGC GGAAAGCACT GGAAGAAGCA GGTTTCAAGC 
     2941 TGCCCTGTCA ACTTCATCAA GTCATCGTTG CCCGGTTTGC AGACGACGAG CTAATCATCG 
     3001 ACTTTGACAA TTTTGTGCGG TGTTTGGTCC GTCTGGAAAC GCTATTCAAG ATATTCAAGC 
     3061 AGCTAGACCC TGAGAACACT GGAACGATAC AGCTCAACCT TGCTTCGTGG CTGAGTTTTT 
     3121 CAGTACTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3181 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3241 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3301 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3361 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3421 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3481 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3541 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3601 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3661 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3721 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3781 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3841 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3901 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3961 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4021 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4081 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4141 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4201 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4261 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4321 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4381 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4441 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4501 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4561 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4621 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4681 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4741 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4801 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4861 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4921 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4981 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5041 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5101 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5161 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5221 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5281 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5341 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5401 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5461 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5521 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5581 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5641 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5701 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5761 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5821 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5881 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5941 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6001 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6061 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6121 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6181 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6241 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6301 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6361 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6421 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6481 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6541 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6601 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6661 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6721 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6781 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6841 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6901 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6961 CAAAAATTTA ACGCGAATTT T
//