LOCUS       MR210118                6978 bp    DNA     circular  SYN 
DEFINITION  Col8a2 (Myc-DDK-tagged) - Mouse collagen, type VIII, alpha 2 (Col8a2)
ACCESSION   NM_199473
VERSION     NM_199473.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3135..3226 
                     /label="Myc-DDK"  
     misc_feature    3256..3841 
                     /label="PolyA signal"  
     misc_feature    3313..3332 
                     /label="Restriction Enzyme"  
     misc_feature    4117..4789 
                     /label="ColE1"  
     misc_feature    5219..6013 
                     /label="Kan/Neo"  
     misc_feature    6048..6350 
                     /label="SV40 ori"  
     misc_feature    6436..6913 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3117..3123 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3117 
                     /label="Col8a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGGGGCT CTGATGCCCC TGCCTTCGCT GCTGCTGTTG CTGTTGGGGT 
     1081 GTGGGCCAAG AGTGTCCTCT GGCGGCGGAG CTGGGGGTGC AGCGGGCTAT GCCCCGGTAA 
     1141 AGTATGTGCA GCCCATGCAG AAAGGACCCG TGGGACCGCC TTTCCGAGAG GGCAAAGGCC 
     1201 AGTACTTGGA AATGCCTCTA CCGATGCTGC CAATGGACTT GAAAGGAGAG CCAGGTCCCC 
     1261 CTGGGAAGCC GGGACCTCGG GGTCCCCCTG GGCCTCCTGG CTTCCCAGGA AAACCAGGCA 
     1321 CTGGAAAACC TGGGGTTCAT GGACAGCCAG GCCCTGCTGG CCCCCCTGGC TTCTCCCGAA 
     1381 TGGGCAAAGC TGGTCCCCCA GGGCTCCCAG GCAAGGTTGG ACCACCAGGG CAGCCAGGGC 
     1441 TACGGGGGGA GCCAGGGATA CGAGGGGACC AGGGCCTCCG GGGGCCCCCA GGACCCCCTG 
     1501 GCCTTCCTGG TCCCTCAGGT ATTACTGTCC CTGGAAAACC AGGTGCCCAG GGGGCTCCAG 
     1561 GGCCTCCAGG ATTTAGGGGG GAGCCAGGAC CCCAGGGAGA GCCTGGGCCC CGAGGAGATA 
     1621 GGGGCCTCAA AGGGGATAAT GGAGTGGGCC AACCAGGGCT GCCTGGAGCC CCTGGACAGG 
     1681 CAGGTGCCCC TGGGCCCCCT GGGCTGCCCG GTCCAGCTGG CTTGGGCAAA CCAGGTTTGG 
     1741 ATGGGATACC AGGAGCCCCA GGAGACAAAG GTGATTCTGG CCCCCCTGGG GTTCCAGGTT 
     1801 CTAGGGGAGA GCCAGGAGCT GTGGGTCCAA AAGGCCCTCC TGGGGTAGAT GGTGTGGGGA 
     1861 TACCAGGGGC AGCAGGGGTG CCAGGGCCAC AGGGTCCAGT AGGGGCTAAA GGAGAGCCAG 
     1921 GGCTCCGGGG CCCCCCTGGC CTGATAGGCC CTGTTGGCTA TGGGATGCCA GGAAAACCAG 
     1981 GACCTAAGGG AGACAGGGGC CCAGTTGGGG CCCCAGGGCT CTTAGGAGAC AGAGGTGAGC 
     2041 CAGGAGAGGA TGGGAAGCCA GGGGAGCAGG GTCCACAGGG ACTTGGGGGT CCACCTGGCC 
     2101 TTCCTGGGTC TGCAGGGCTT CCTGGTAGAC GTGGGCCCCC TGGGTCAAAG GGAGAGGTAG 
     2161 GACCTGGAGG TCCCCCAGGA GTGCCTGGCA TTCGAGGAGA CCAAGGGCCT AATGGCCTGG 
     2221 CTGGGAAGCC TGGGCTCCCT GGTGAGAGGG GACTTCCTGG GGCCCATGGA CCCCCTGGAC 
     2281 CGACTGGGCC TAAGGGTGAG CCAGGTTTTA CAGGTCGCCC TGGAGGGCCA GGGGTGGCAG 
     2341 GAGCCCTAGG ACAGAAAGGT GACTTGGGGC TTCCTGGGCA GCCTGGCTTG AGGGGTCCCT 
     2401 CTGGAATCCC AGGGCTCCAG GGCCCAGCTG GCCCTATTGG GCCTCAGGGT CTGCCAGGCC 
     2461 TGAAGGGTGA ACCGGGCCTT CCAGGGCCTC CTGGAGAGGG AAAAGTAGGG GAGCCTGGCT 
     2521 CTGCTGGTCC CACAGGGCCC CCTGGAGTCC CTGGCTCCCC AGGACTCACA GGTCCTCCTG 
     2581 GGCCCCCTGG GCCTCCTGGG CCTCCTGGTG CCCCCGGAGC CCTGGATGAG ACTGGTATCG 
     2641 CAGGCTTGCA TCTGCCCAAT GGTGGCGTGG AGGGAGCTGT ACTGGGCAAA GGAGGCAAGC 
     2701 CACAGTTTGG GCTCGGTGAG CTGTCAGCCC ACGCCACTCC AGCCTTTACT GCAGTGCTCA 
     2761 CTTCCCCCTT CCCTGCCTCA GGCATGCCGG TTAGGTTTGA CCGGACTCTC TACAATGGCC 
     2821 ACAGCGGCTA CAACCCAGCC ACTGGCATCT TCACCTGCCC CGTGGGAGGG GTCTACTACT 
     2881 TTGCATACCA TGTGCATGTC AAGGGCACCA ATGTGTGGGT AGCCCTGTAC AAGAACAATG 
     2941 TGCCGGCCAC CTACACGTAC GACGAATACA AGAAGGGCTA CCTGGACCAG GCTTCGGGAG 
     3001 GGGCTGTGCT CCAGCTGCGG CCCAATGACC AGGTGTGGGT GCAGATGCCC TCAGATCAGG 
     3061 CCAATGGTCT CTACTCCACC GAGTACATCC ATTCGTCCTT CTCAGGATTC TTGCTCTGTC 
     3121 CCACAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3181 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3241 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3301 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3361 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3421 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3481 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3541 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3601 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3661 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3721 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3781 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3841 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3901 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3961 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4021 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4081 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4141 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4201 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4261 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4321 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4381 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4441 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4501 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4561 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4621 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4681 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4741 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4801 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4861 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4921 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4981 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5041 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5101 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5161 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5221 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5281 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5341 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5401 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5461 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5521 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5581 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5641 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5701 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5761 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5821 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5881 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5941 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6001 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6061 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6121 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6181 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6241 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6301 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6361 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6421 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6481 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6541 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6601 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6661 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6721 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6781 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6841 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6901 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6961 AAATTTAACG CGAATTTT
//