LOCUS       MR210073                6957 bp    DNA     circular  SYN 
DEFINITION  Prmt7 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7)
ACCESSION   NM_145404
VERSION     NM_145404.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3114..3205 
                     /label="Myc-DDK"  
     misc_feature    3235..3820 
                     /label="PolyA signal"  
     misc_feature    3292..3311 
                     /label="Restriction Enzyme"  
     misc_feature    4096..4768 
                     /label="ColE1"  
     misc_feature    5198..5992 
                     /label="Kan/Neo"  
     misc_feature    6027..6329 
                     /label="SV40 ori"  
     misc_feature    6415..6892 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3096..3102 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3096 
                     /label="Prmt7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTCTTC TGTGGCCGTG CCAATCCTAC CACGGGATCC CTGGAGTGGC 
     1081 TGGAGGAGGA TGAACACTAT GATTACCACC AGGAGATTGC CAGGTCATCC TATGCCGACA 
     1141 TGCTACATGA CAAAGACAGA AATATAAAAT ACTACCAGGG TATCCGGGCA GCTGTGAGCA 
     1201 GGGTGAAAGA CAGAGGACAG AAGGCCTTGG TTCTTGACAT TGGCACTGGC ACAGGCCTCT 
     1261 TGTCAATGAT GGCAGTTACT GCAGGGGCTG ACTTCTGCTA TGCTATCGAG GTTTTTAAGC 
     1321 CTATGGCTGA GGCTGCTGTG AAGATTGTGG AGAGGAATGG CTTCAGTGAT AAGATTAAAG 
     1381 TCATTAACAA GCACTCCACT GAGGTGACAG TCGGACCAGA TGGTGACTTG CCGTGTCGTG 
     1441 CTAACATTCT GATCACGGAG CTGTTTGACA CAGAGCTGAT TGGGGAGGGA GCGCTGCCCT 
     1501 CTTATGAGCA TGCACACAAG CATCTTGTCC AGGAAGACTG CGAGGCAGTG CCACACAGGG 
     1561 CAACTGTCTA TGCCCAGCTG GTGGAGTCCC GAAGGATGTG GTCCTGGAAC AAGCTGTTTC 
     1621 CCGTCCGTGT CCGGACGAGT CTAGGCGAGC AGGTCATCGT CCCCCCCTCA GAATTGGAGA 
     1681 GGTGTCCTGG TGCGCCTTCA GTCTGTGACA TTCAGCTGAA CCAGGTGTCG CCTGCTGACT 
     1741 TCACTGTCCT CAGTGATGTG CTGCCAATGT TCAGCGTGGA CTTCAGCAAG CAAGTCAGCA 
     1801 GCTCGGCAGC GTGCCATAGC AGGCAGTTTG TACCTTTGGC GTCTGGCCAA GCACAGGTGG 
     1861 TTCTGTCCTG GTGGGACATT GAAATGGACC CTGAGGGCAA GATCAAGTGC ACCATGGCAC 
     1921 CCTTTTGGGC ACAGACAGAT CCGCAGGAGC TTCAGTGGCG GGACCACTGG ATGCAGTGTG 
     1981 TGTACTTCCT GCCGCAGGAG GAGCCTGTTG TGCAGGGCTC ACCCCGGTGC CTGGTAGCCC 
     2041 ACCATGATGA CTACTGTGTG TGGTACAGCC TTCAGAGAAC CAGCCCTGAT GAGAACGACA 
     2101 GCGCCTACCA AGTGCGACCT GTGTGTGACT GTCAGGCTCA CTTGCTCTGG AACCGGCCTC 
     2161 GGTTTGGAGA AATCAATGAT CAGGACAGAA CTGATCACTA TGCCCAGGCC CTGAGGACTG 
     2221 TGCTGCTGCC AGGTAGCGTC TGCCTTTGTG TGAGTGATGG CAGTCTCCTC TCCATGCTGG 
     2281 CCCATCACCT CGGAGCGGAG CAGGTGTTTA CAGTTGAGAG TTCAGTAGCT TCCTATAGAC 
     2341 TGATGAAAAG GATCTTCAAG GTTAACCACT TGGAAGATAA AATCAGTGTC ATCAATAAAC 
     2401 GGCCTGAGTT GCTGACAGCT GCAGACCTGG AGGGCAAGAA GGTCTCCCTC CTCCTGGGTG 
     2461 AACCCTTTTT CACCACCAGC CTGCTGCCAT GGCACAACCT GTACTTCTGG TATGTCCGTA 
     2521 CCTCTGTGGA CCAGCACCTA GCACCTGGAG CTGTGGTGAT GCCTCAGGCT GCCTCACTGC 
     2581 ATGCCGTGAT TGTGGAGTTC AGGGACCTGT GGCGGATCCG GAGTCCTTGC GGTGACTGCG 
     2641 AAGGTTTTGA TGTGCACATC ATGGATGATA TGATCAAGCA CTCCCTGGAT TTCCGAGAGA 
     2701 GCAGAGAGGC AGAGCCACAC CCACTGTGGG AATACCCCTG CAGAAGCCTC TCCAAGCCTC 
     2761 AAGAGATCCT GACTTTTGAT TTCCAGCAGC CCATCCCCCA ACAGCCTATG CAATCCAAGG 
     2821 GCACAATGGA GCTGACAAGA CCCGGGAAGA GCCATGGGGC TGTCCTGTGG ATGGAGTATC 
     2881 AGCTCACTCC AGACAGCACG ATCAGCACTG GCCTCATAAA CCCTGCAGAA GACAAGGGGG 
     2941 ACTGCTGCTG GAACCCCCAC TGCAAGCAAG CTGTGTACTT CCTCAGCACC ACGCTGGATC 
     3001 TCAGAGTGCC TCTGAATGGC CCTCGGTCAG TCAGCTATGT TGTGGAGTTT CACCCCCTCA 
     3061 CTGGAGACAT CACCATGGAG TTTAGGCTTG CAGACACCTT GAGCACGCGT ACGCGGCCGC 
     3121 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3181 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3241 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3301 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3361 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3421 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3481 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3541 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3601 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3661 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3721 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3781 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3841 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3901 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     3961 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4021 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4081 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4141 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4201 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4261 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4321 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4381 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4441 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4501 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4561 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4621 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4681 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4741 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4801 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4861 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4921 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     4981 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5041 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5101 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5161 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5221 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5281 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5341 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5401 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5461 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5521 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5581 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5641 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5701 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5761 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5821 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5881 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     5941 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6001 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6061 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6121 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6181 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6241 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6301 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6361 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6421 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6481 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6541 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6601 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6661 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6721 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6781 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6841 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6901 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//