LOCUS       MR209829                6858 bp    DNA     circular  SYN 
DEFINITION  Btk (Myc-DDK-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk)
ACCESSION   NM_013482
VERSION     NM_013482.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3015..3106 
                     /label="Myc-DDK"  
     misc_feature    3136..3721 
                     /label="PolyA signal"  
     misc_feature    3193..3212 
                     /label="Restriction Enzyme"  
     misc_feature    3997..4669 
                     /label="ColE1"  
     misc_feature    5099..5893 
                     /label="Kan/Neo"  
     misc_feature    5928..6230 
                     /label="SV40 ori"  
     misc_feature    6316..6793 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2997..3003 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2997 
                     /label="Btk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCAGTG ATACTGGAGA GCATCTTTCT GAAGCGCTCC CAGCAGAAAA 
     1081 AGAAAACATC ACCTTTAAAC TTCAAGAAGC GCCTGTTTCT CTTGACTGTA CACAAACTTT 
     1141 CATACTATGA ATATGACTTT GAACGTGGGA GAAGAGGCAG TAAGAAAGGT TCAATAGATG 
     1201 TTGAGAAGAT CACCTGTGTT GAAACAGTAA TTCCTGAAAA AAATCCCCCA CCAGAAAGAC 
     1261 AGATTCCGAG GAGAGGTGAG GAGTCTAGTG AAATGGAACA GATTTCAATC ATTGAAAGGT 
     1321 TCCCGTACCC ATTCCAGGTT GTATATGATG AAGGACCTCT CTATGTTTTC TCCCCAACTG 
     1381 AAGAGCTGAG AAAGCGCTGG ATTCACCAGC TCAAAAATGT AATCCGGTAC AATAGTGACC 
     1441 TGGTACAGAA ATACCATCCT TGCTTCTGGA TTGATGGACA GTATCTCTGC TGCTCTCAGA 
     1501 CAGCCAAGAA TGCTATGGGC TGCCAAATTT TGGAGAACAG GAATGGAAGC TTAAAACCTG 
     1561 GGAGTTCTCA TCGAAAAACG AAAAAGCCTC TTCCCCCTAC CCCAGAGGAA GATCAGATCT 
     1621 TGAAAAAACC GCTTCCCCCG GAGCCAACAG CAGCACCAAT CTCCACAACC GAGCTGAAAA 
     1681 AGGTCGTGGC CCTTTATGAT TACATGCCAA TGAACGCAAA TGACTTACAA TTGCGAAAGG 
     1741 GCGAGGAGTA TTTTATCCTG GAGGAGAGCA ACCTACCGTG GTGGCGAGCA CGAGATAAAA 
     1801 ATGGGCAGGA AGGCTACATC CCAAGTAACT ATATCACTGA AGCTGAGGAC TCCATAGAGA 
     1861 TGTATGAGTG GTATTCCAAG CACATGACTC GAAGTCAAGC TGAGCAACTG CTAAAGCAAG 
     1921 AGGGGAAAGA AGGAGGTTTC ATTGTCAGAG ACTCCAGCAA AGCTGGAAAA TACACCGTGT 
     1981 CTGTGTTTGC TAAATCTACT GGGGAGCCTC AAGGGGTGAT CCGCCATTAC GTTGTGTGTT 
     2041 CCACGCCACA GAGCCAGTAT TACCTGGCTG AGAAACACCT CTTCAGCACC ATCCCTGAGC 
     2101 TCATTAACTA CCATCAACAC AACTCTGCAG GCCTCATATC CAGGCTGAAA TATCCTGTGT 
     2161 CTAAACAAAA CAAAAACGCG CCTTCTACTG CAGGCCTGGG CTATGGATCA TGGGAAATTG 
     2221 ATCCAAAGGA CCTCACCTTC TTGAAGGAGC TTGGGACTGG ACAATTCGGT GTCGTGAAAT 
     2281 ATGGGAAGTG GAGGGGCCAA TATGATGTGG CCATCAAGAT GATCAGAGAA GGTTCCATGT 
     2341 CGGAGGATGA ATTCATTGAA GAAGCCAAAG TCATGATGAA TCTTTCCCAT GAGAAGCTGG 
     2401 TGCAGTTGTA TGGCGTCTGC ACCAAACAAC GCCCCATCTT CATCATCACC GAGTACATGG 
     2461 CTAATGGCTG CCTCTTGAAC TACCTGAGGG AGATGCGGCA CCGCTTCCAG ACACAGCAGC 
     2521 TGCTTGAGAT GTGCAAAGAT GTCTGTGAAG CAATGGAATA CTTGGAGTCG AAGCAGTTCC 
     2581 TTCACAGAGA CCTGGCAGCT CGAAACTGTT TGGTAAACGA TCAAGGAGTT GTGAAAGTAT 
     2641 CTGACTTTGG CCTGTCTAGG TATGTCCTTG ATGATGAGTA CACCAGCTCT GTAGGCTCCA 
     2701 AGTTTCCAGT CCGGTGGTCT CCACCAGAAG TGCTTATGTA TAGCAAGTTC AGCAGCAAAT 
     2761 CTGACATCTG GGCTTTTGGG GTTTTAATGT GGGAGATCTA CTCCCTGGGG AAGATGCCGT 
     2821 ATGAGAGATT TACTAACAGT GAGACAGCAG AACACATTGC TCAAGGCTTA CGTCTCTACA 
     2881 GGCCTCATCT GGCATCAGAG AGGGTATATA CCATCATGTA CAGCTGCTGG CACGAGAAAG 
     2941 CAGATGAACG TCCTAGTTTC AAAATTCTCT TGAGTAACAT TCTAGATGTG ATGGATGAAG 
     3001 AATCCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3061 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3121 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3181 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3241 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3301 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3361 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3421 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3481 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3541 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3601 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3661 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3721 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3781 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3841 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     3901 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     3961 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4021 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4081 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4141 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4201 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4261 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4321 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4381 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4441 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4501 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4561 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4621 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4681 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4741 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4801 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4861 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     4921 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     4981 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5041 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5101 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5161 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5221 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5281 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5341 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5401 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5461 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5521 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5581 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5641 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5701 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5761 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5821 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     5881 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     5941 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6001 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6061 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6121 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6181 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6241 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6301 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6361 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6421 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6481 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6541 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6601 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6661 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6721 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6781 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6841 AAATTTAACG CGAATTTT
//