LOCUS       MR209737                6825 bp    DNA     circular  SYN 
DEFINITION  Raf1 (Myc-DDK-tagged) - Mouse v-raf-leukemia viral oncogene 1 (Raf1)
ACCESSION   NM_029780
VERSION     NM_029780.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2982..3073 
                     /label="Myc-DDK"  
     misc_feature    3103..3688 
                     /label="PolyA signal"  
     misc_feature    3160..3179 
                     /label="Restriction Enzyme"  
     misc_feature    3964..4636 
                     /label="ColE1"  
     misc_feature    5066..5860 
                     /label="Kan/Neo"  
     misc_feature    5895..6197 
                     /label="SV40 ori"  
     misc_feature    6283..6760 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2964..2970 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2964 
                     /label="Raf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCACATA CAGGGAGCTT GGAAGACGAT CAGCAATGGC TTTGGACTCA 
     1081 AAGATGCGGT GTTTGATGGC TCCAGCTGCA TCTCCCCTAC CATTGTTCAG CAGTTTGGCT 
     1141 ATCAGCGCCG GGCCTCAGAT GATGGCAAGC TCACGGATTC TTCTAAGACA AGCAATACTA 
     1201 TCCGGGTTTT CTTGCCGAAT AAGCAAAGGA CTGTGGTCAA TGTGCGGAAT GGAATGAGCT 
     1261 TACATGACTG CCTTATGAAA GCTCTGAAGG TGAGAGGCCT GCAGCCAGAG TGCTGTGCAG 
     1321 TGTTCAGACT TCTCCAGGAA CACAAAGGTA AGAAAGCACG CTTAGATTGG AACACCGATG 
     1381 CCGCCTCTCT GATTGGAGAA GAACTGCAAG TGGATTTTTT GGATCATGTT CCACTCACAA 
     1441 CTCACAACTT TGCTCGGAAA ACGTTCCTGA AGCTTGCATT CTGTGACATC TGTCAGAAGT 
     1501 TCCTGCTAAA TGGATTTCGA TGTCAGACTT GTGGCTACAA GTTTCATGAG CACTGTAGCA 
     1561 CCAAAGTACC TACTATGTGT GTGGACTGGA GTAATATCAG ACAGCTCTTG CTGTTTCCAA 
     1621 ATTCCACTGT TGGTGACAGT GGAGTCCCAG CACCACCTTC TTTCCCAATG CGTCGGATGC 
     1681 GAGAATCTGT TTCCCGGATG CCTGCTAGTT CCCAGCACAG ATACTCTACA CCCCATGCCT 
     1741 TCACTTTCAA CACCTCCAGC CCTTCCTCAG AAGGTTCCCT GTCCCAGAGG CAGAGGTCAA 
     1801 CGTCCACTCC CAATGTCCAC ATGGTCAGCA CCACCCTGCA TGTGGACAGC AGGATGATTG 
     1861 AGGATGCAAT TCGAAGTCAC AGTGAATCAG CCTCACCTTC AGCCCTGTCC AGCAGCCCAA 
     1921 ACAACCTGAG TCCAACAGGC TGGTCACAGC CCAAAACCCC TGTGCCAGCA CAAAGAGAGC 
     1981 GGGCACCAGG ATCTGGGACC CAGGAAAAAA ACAAAATTAG GCCTCGTGGG CAGAGAGACT 
     2041 CGAGTTATTA CTGGGAAATA GAAGCCAGTG AGGTGATGCT GTCTACTCGG ATCGGGTCAG 
     2101 GTTCCTTTGG CACTGTGTAC AAGGGCAAGT GGCATGGAGA TGTTGCAGTA AAGATCCTAA 
     2161 AGGTGGTTGA CCCAACTCCA GAGCAACTTC AGGCCTTCAG GAACGAGGTG GCTGTTTTGC 
     2221 GCAAAACACG GCATGTTAAC ATCCTGCTGT TCATGGGGTA CATGACAAAG GACAACCTGG 
     2281 CGATTGTGAC TCAGTGGTGT GAAGGCAGCA GTCTCTACAA ACACCTGCAT GTCCAGGAGA 
     2341 CCAAATTCCA GATGTTCCAG CTAATTGACA TTGCCCGACA GACAGCTCAG GGAATGGACT 
     2401 ATTTGCATGC AAAGAACATC ATCCACAGAG ACATGAAATC CAACAATATA TTTCTCCATG 
     2461 AAGGCCTCAC GGTGAAAATT GGAGATTTTG GTTTGGCAAC AGTGAAGTCA CGCTGGAGTG 
     2521 GTTCTCAGCA GGTTGAACAG CCCACTGGCT CTGTGCTGTG GATGGCCCCA GAAGTAATCC 
     2581 GGATGCAGGA TGACAACCCG TTCAGCTTCC AGTCCGACGT GTACTCGTAC GGCATCGTGC 
     2641 TGTACGAGCT GATGGCTGGG GAGCTTCCCT ACGCCCACAT CAACAACCGA GACCAGATCA 
     2701 TCTTCATGGT AGGCCGTGGG TATGCATCCC CTGATCTCAG CAGGCTCTAC AAGAACTGCC 
     2761 CCAAGGCAAT GAAGAGGTTG GTGGCTGACT GTGTGAAGAA AGTCAAAGAA GAGAGACCTT 
     2821 TGTTTCCCCA GATCCTGTCT TCCATCGAGC TGCTTCAGCA CTCTCTGCCG AAAATCAACA 
     2881 GGAGCGCCTC TGAGCCTTCC CTGCATCGGG CAGCTCACAC TGAGGACATC AATGCTTGCA 
     2941 CGCTGACTAC ATCCCCAAGG CTACCAGTCT TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3001 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3061 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3121 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3181 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3241 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3301 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3361 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3421 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3481 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3541 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3601 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3661 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3721 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3781 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     3841 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     3901 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     3961 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4021 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4081 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4141 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4201 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4261 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4321 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4381 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4441 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4501 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4561 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4621 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4681 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4741 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4801 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     4861 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     4921 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     4981 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5041 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5101 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5161 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5221 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5281 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5341 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5401 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5461 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5521 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5581 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5641 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5701 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5761 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     5821 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     5881 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     5941 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6001 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6061 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6121 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6181 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6241 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6301 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6361 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6421 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6481 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6541 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6601 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6661 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6721 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6781 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//