LOCUS       MR209636                6810 bp    DNA     circular  SYN 
DEFINITION  Idua (Myc-DDK-tagged) - Mouse iduronidase, alpha-L- (Idua)
ACCESSION   NM_008325
VERSION     NM_008325.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2967..3058 
                     /label="Myc-DDK"  
     misc_feature    3088..3673 
                     /label="PolyA signal"  
     misc_feature    3145..3164 
                     /label="Restriction Enzyme"  
     misc_feature    3949..4621 
                     /label="ColE1"  
     misc_feature    5051..5845 
                     /label="Kan/Neo"  
     misc_feature    5880..6182 
                     /label="SV40 ori"  
     misc_feature    6268..6745 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2949..2955 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2949 
                     /label="Idua"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGACCCCCG CGTCCCTCCT CAGCTATGCT GACGTTTTTT GCTGCGTTCT 
     1081 TGGCCGCGCC CTTGGCGCTG GCTGAGTCAC CGTACCTGGT GCGTGTGGAC GCAGCCCGCC 
     1141 CGCTGAGGCC TCTGTTGCCC TTCTGGAGGA GCACCGGCTT CTGCCCCCCA CTGCCTCACG 
     1201 ACCAGGCTGA CCAGTACGAC CTTAGTTGGG ACCAGCAACT GAACCTTGCC TACATAGGTG 
     1261 CCGTACCTCA CAGTGGCATT GAGCAGGTCC GGATACACTG GCTGCTGGAT CTCATCACAG 
     1321 CCAGGAAGTC ACCTGGGCAG GGACTTATGT ACAACTTCAC CCACTTGGAT GCATTCTTGG 
     1381 ACCTTCTCAT GGAGAACCAG CTTCTCCCTG GATTTGAGCT CATGGGCAGT CCTTCTGGGT 
     1441 ACTTCACGGA CTTTGATGAC AAGCAGCAGG TGTTTGAATG GAAGGACCTG GTTTCTCTCT 
     1501 TGGCCAGGAG ATACATTGGT AGGTATGGGC TGACACACGT TTCCAAGTGG AACTTTGAGA 
     1561 CTTGGAATGA ACCAGACCAC CATGACTTTG ACAACGTGTC CATGACCACA CAAGGCTTCC 
     1621 TGAATTACTA TGATGCCTGC TCTGAGGGGC TGCGCATTGC CAGCCCCACT TTGAAGTTGG 
     1681 GTGGTCCTGG GGATTCCTTC CACCCCCTGC CAAGGTCACC AATGTGCTGG AGCCTCCTGG 
     1741 GTCACTGTGC CAATGGAACC AACTTCTTCA CTGGCGAGGT GGGCGTGCGT CTGGATTACA 
     1801 TCTCCCTGCA CAAGAAGGGT GCAGGTAGCT CCATCGCCAT CCTGGAGCAG GAGATGGCAG 
     1861 TTGTGGAGCA GGTCCAGCAG CTCTTCCCTG AGTTCAAGGA TACCCCTATT TACAATGACG 
     1921 AGGCAGACCC TCTGGTGGGC TGGTCCCTGC CACAACCTTG GAGAGCTGAT GTGACTTATG 
     1981 CGGCCCTGGT GGTGAAGGTC ATTGCACAGC ACCAGAACCT GCTGTTTGCC AACAGCAGTT 
     2041 CCTCCATGCG CTATGTGCTC CTCAGCAATG ACAATGCCTT CCTGAGCTAC CACCCGTACC 
     2101 CTTTCTCCCA GCGCACACTT ACTGCTCGAT TCCAGGTCAA CAATACTCAC CCACCCCACG 
     2161 TGCAGTTGCT GCGAAAGCCA GTACTCACAG TCATGGGGCT CATGGCCCTG TTGGATGGAG 
     2221 AACAACTCTG GGCAGAGGTC TCAAAGGCTG GGGCTGTGTT GGACAGCAAT CATACAGTGG 
     2281 GTGTCCTGGC CAGCACCCAT CACCCTGAAG GCTCCGCAGC GGCCTGGAGT ACCACAGTCC 
     2341 TCATCTACAC TAGTGATGAC ACCCACGCAC ACCCCAACCA CAGTATCCCT GTGACTCTTC 
     2401 GCCTGCGTGG GGTACCTCCT GGCTTGGATC TTGTCTACAT AGTACTCTAC TTAGACAATC 
     2461 AACTCAGCAG CCCCTACAGT GCGTGGCAGC ACATGGGCCA GCCAGTCTTC CCCTCTGCAG 
     2521 AGCAGTTCCG ACGTATGCGC ATGGTGGAGG ACCCCGTGGC TGAGGCACCA CGCCCCTTTC 
     2581 CTGCTAGGGG CCGCCTGACC CTACACCGGA AGCTTCCGGT GCCATCACTC CTGCTGGTGC 
     2641 ATGTATGCAC ACGCCCCTTG AAGCCACCTG GGCAGGTCAG CCGGCTCCGT GCACTGCCCC 
     2701 TGACACATGG ACAGCTGATT TTGGTCTGGT CAGATGAGCG TGTGGGCTCC AAGTGCCTGT 
     2761 GGACATATGA GATCCAGTTT TCCCAGAAAG GTGAAGAGTA TGCCCCAATC AACAGGAGGC 
     2821 CGTCTACTTT TAACCTCTTT GTGTTCAGCC CAGACACAGC TGTGGTCTCT GGCTCCTACC 
     2881 GAGTTCGAGC ATTGGATTAC TGGGCCCGGC CAGGCCCCTT CTCCGACCCT GTGACTTACC 
     2941 TGGATGTCCC TGCCTCAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3001 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3061 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3121 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3181 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3241 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3301 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3361 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3421 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3481 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3541 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3601 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3661 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     3721 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     3781 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     3841 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     3901 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     3961 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4021 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4081 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4141 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4201 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4261 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4321 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4381 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4441 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4501 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4561 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4621 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4681 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     4741 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     4801 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     4861 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     4921 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     4981 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5041 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5101 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5161 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5221 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5281 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5341 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5401 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5461 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5521 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5581 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5641 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     5701 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     5761 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     5821 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     5881 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     5941 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6001 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6061 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6121 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6181 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6241 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6301 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6361 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6421 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6481 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6541 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6601 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6661 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     6721 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     6781 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//