LOCUS       MR209429                7158 bp    DNA     circular  SYN 
DEFINITION  Cul4a (Myc-DDK-tagged) - Mouse cullin 4A (Cul4a)
ACCESSION   NM_146207
VERSION     NM_146207.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3315..3406 
                     /label="Myc-DDK"  
     misc_feature    3436..4021 
                     /label="PolyA signal"  
     misc_feature    3493..3512 
                     /label="Restriction Enzyme"  
     misc_feature    4297..4969 
                     /label="ColE1"  
     misc_feature    5399..6193 
                     /label="Kan/Neo"  
     misc_feature    6228..6530 
                     /label="SV40 ori"  
     misc_feature    6616..7093 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3297..3303 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3297 
                     /label="Cul4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGACGAG GGCCCTCGGA AGGGCAGCGT CTCGGCTCTC ATGGGCCGCA 
     1081 CCAACGGCCT CACCAAGCCC GCGGCGTTGG CCGGTGGCCC CGCCAAGCCG GGGGGCACGG 
     1141 GAGGCTCCAG GAAGCTGGTC ATCAAGAACT TCCGAGACAG GCCGCGGCTG CCTGACAACT 
     1201 ACACTCAGGA CACGTGGCGG AAGCTTCACG AAGCGGTCAA GGCCATCCAG AGCAGCACAT 
     1261 CCATCAGGTA CAACCTGGAG GAGCTGTACC AGGCTGTTGA AAATCTTTGT TCTCACAAAG 
     1321 TCTCCCCAAC GCTCTACAAG CAGCTGCGCC AGGTCTGTGA AGACCATGTC CAGGCCCAGA 
     1381 TCCTCCCATT CAGAGAAGAC TCACTAGACA GCGTTTTATT TCTAAAGAAG ATTAACACAT 
     1441 GCTGGCAAGA TCACTGCAGA CAAATGATCA TGATCAGAAG CATCTTCCTG TTTCTGGATC 
     1501 GAACCTACGT CCTCCAGAAT TCCATGCTGC CTTCTATCTG GGACATGGGC CTAGAGCTGT 
     1561 TTAGGAACCA CATCATCAGC GACAGGATGG TGCAGAGTAA GACCATTGAT GGGATCCTGC 
     1621 TGCTGATTGG GCGTGAACGA AGTGGAGAGG CTGTGGACAG GAGTCTGCTT CGGAGTTTGC 
     1681 TAAGCATGCT CTCAGATCTC CAGGTGTACA AAGACTCATT TGAGCTGAAG TTCTTGGAAG 
     1741 AGACTAACTG TCTATACGCT GCTGAAGGCC AGAGGCTGAT GCAGGACAGG GAGGTTCCAG 
     1801 AATACCTTAA CCATGTGAGC AAACGTCTAG AAGAGGAAGC AGACCGAGTG ATCACCTACT 
     1861 TAGACCACAG CACACAAAAA CCGTTGATTG CCTGTGTGGA GAAACAGCTG TTAGGAGAAC 
     1921 ATTTGACAGC AATTCTACAG AAAGGCCTAG AGCACCTGCT GGATGAGAAC AGGGTGCCCG 
     1981 ACCTCACCCA GATGTACCAG CTCTTCAGCC GGGTGAAAGG AGGGCAGCAT GCGCTGCTGC 
     2041 AGCACTGGAG CGAGTACATC AAGACCTTTG GAACAACCAT TGTCATCAAT CCTGAGAAAG 
     2101 ACAAAGACAT GGTCCAAGAC CTGCTGGACT TTAAGGACAA AGTGGACCAC GTGGTGGAGG 
     2161 TGTGCTTCCA GAGGAACGAG CGCTTCATCA ACCTGATGAA GGAGTCCTTC GAGACGTTCA 
     2221 TCAACAAGAG ACCAAACAAG CCTGCAGAGC TCATTGCCAA GCACGTGGAC TCAAAGTTAC 
     2281 GGGCCGGCAA CAAAGAAGCC ACAGATGAGG AACTGGAGAG GATCCTGGAC AAGATCATGA 
     2341 TTCTCTTCCG TTTCATCCAT GGTAAAGATG TCTTTGAAGC GTTTTATAAG AAAGACTTAG 
     2401 CAAAAAGGCT GCTGGTGGGG AAGAGCGCGT CGGTGGACGC GGAGAAGTCC ATGCTGTCGA 
     2461 AGCTCAAGCA CGAGTGCGGA GCTGCGTTTA CCAGCAAACT GGAGGGCATG TTCAAGGACA 
     2521 TGGAGCTCTC CAAAGACATC ATGGTTCACT TCAAGCAGCA TATGCAGAAC CAGAGTGCGC 
     2581 CAGGCCCCAT TGACCTCACG GTGAACATAC TCACCATGGG CTACTGGCCC ACGTACACAC 
     2641 CCATGGAAGT GCACCTCCCT CCAGAGATGG TCAGACTTCA GGAAGTATTC AAGACGTTCT 
     2701 ACCTGGGCAA GCACAGTGGG CGGAAGCTGC AATGGCAGAC AACGCTGGGA CACGCCGTGC 
     2761 TGAAGGCGGA CTTTAAGGAG GGGAAGAAGG AGTTCCAGGT ATCCCTCTTC CAGACGCTGG 
     2821 TGCTGCTCAT GTTCAACGAA GGGGATGGAT TTAGCTTTGA AGAAATAAAA ATGGCCACTG 
     2881 GCATAGAGGA CAGTGAGTTG CGAAGAACAC TACAGTCTCT GGCCTGTGGT AAAGCACGGG 
     2941 TCCTAATTAA AAGTCCCAAA GGAAAGGAGG TGGAAGATGG AGATAAGTTC ATCTTCAATG 
     3001 CAGACTTCAA ACACAAGTTG TTCAGAATAA AGATCAATCA AATCCAGATG AAGGAGACCG 
     3061 TTGAAGAACA GGTTAGCACT ACAGAGAGAG TATTCCAGGA TAGACAGTAC CAGATCGACG 
     3121 CTGCTATAGT CAGAATAATG AAAATGAGGA AGACACTTGG TCATAATCTT CTAGTTTCTG 
     3181 AATTATACAA TCAGCTGAAA TTTCCAGTGA AGCCTGGAGA TTTGAAAAAA AGGATTGAGT 
     3241 CACTTATAGA CAGAGACTAT ATGGAACGAG ACAAAGACAG TCCAAATCAG TACCACTACG 
     3301 TGGCAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3361 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3421 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3481 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3541 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3601 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3661 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3721 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3781 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3841 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3901 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3961 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4021 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4081 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4141 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4201 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4261 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4321 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4381 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4441 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4501 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4561 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4621 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4681 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4741 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4801 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4861 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4921 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4981 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5041 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5101 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5161 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5221 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5281 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5341 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5401 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5461 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5521 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5581 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5641 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5701 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5761 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5821 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5881 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5941 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6001 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6061 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6121 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6181 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6241 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6301 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6361 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6481 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6541 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6601 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6661 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6721 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6781 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6841 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6901 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6961 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7021 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7081 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7141 AAATTTAACG CGAATTTT
//