LOCUS       MR209420                6720 bp    DNA     circular  SYN 
DEFINITION  Spast (Myc-DDK-tagged) - Mouse spastin (Spast), transcript variant 2
ACCESSION   NM_016962
VERSION     NM_016962.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2877..2968 
                     /label="Myc-DDK"  
     misc_feature    2998..3583 
                     /label="PolyA signal"  
     misc_feature    3055..3074 
                     /label="Restriction Enzyme"  
     misc_feature    3859..4531 
                     /label="ColE1"  
     misc_feature    4961..5755 
                     /label="Kan/Neo"  
     misc_feature    5790..6092 
                     /label="SV40 ori"  
     misc_feature    6178..6655 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2859..2865 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2859 
                     /label="Spast"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTCCG GCCGGACGAC GGAAGAAGAA AGGCTCGGGC GGCGCGAGCC 
     1081 CGGCGCCCGC CAGGCCTCCG CCCCCCGCCG CGGTCCCCGC CCCTGCCGCC GGCCCGGCCC 
     1141 CTGCGGCCGG CTCGCCGCCT AAGCGGAACC CGTCTTCTTT CTCGTCCCCG CTGGTCGTCG 
     1201 GCTTCGCCCT GCTGCGCCTG CTGGCCTGCC ACCTGGGGCT CCTCTTCGCG TGGCTCTGCC 
     1261 AGCGCTTCTC CCGCGCCCTC ATGGCCGCCA AGAGGAGCTC CGGGACCGCG CCGGCGCCCG 
     1321 CCTCGCCCTC GCCCCCAGAG CCCGGACCGG GTGGCGAGGC CGAGAGCGTC CGCGTCTTCC 
     1381 ACAAGCAGGC CTTCGAGTAC ATCTCCATTG CCCTGCGCAT CGACGAGGAA GAGAAAGGAC 
     1441 AGAAGGAACA AGCTGTGGAA TGGTATAAGA AAGGTATCGA AGAACTGGAA AAAGGAATCG 
     1501 CTGTTATAGT TACGGGCCAA GGTGAACAGT ATGAAAGAGC TAGACGTCTT CAAGCCAAAA 
     1561 TGATGACTAA TTTAGTTATG GCCAAGGACC GTTTACAACT TCTAGAGAAG CTGCAACCAG 
     1621 TTTTGCAATT TTCCAAGTCA CAGACGGACG TCTATAACGA GAGTACTAAC CTGACATGCC 
     1681 GCAATGGACA TCTCCAGTCA GAAAGTGGAG CAGTTCCGAA GAGGAAAGAC CCCTTAACAC 
     1741 ATGCTAGTAA TTCATTGCCT CGATCAAAAA CTGTCCTGAA AAGTGGCTCC GCAGGGCTCT 
     1801 CCGGTCACCA CAGGGCGCCT AGTTGCAGTG GTTTGTCCAT GGTTTCTGGA GCAAGACCGG 
     1861 GACCTGGTCC TGCAGCTACC ACACATAAGG GTACTCCAAA ACCAAATAGA ACCAACAAAC 
     1921 CTTCTACTCC CACAACTGCA GTTCGGAAAA AGAAAGACTT GAAAAATTTT AGGAATGTGG 
     1981 ACAGCAATCT TGCTAACCTT ATAATGAATG AAATTGTTGA CAATGGGACA GCTGTTAAGT 
     2041 TTGATGACAT AGCCGGGCAG GAGCTGGCAA AGCAAGCGCT GCAGGAGATT GTCATCCTTC 
     2101 CTTCTCTGCG GCCTGAGTTG TTCACAGGGC TCAGAGCTCC TGCTAGAGGC TTGTTACTCT 
     2161 TCGGTCCGCC AGGAAACGGA AAAACAATGC TGGCTAAAGC AGTAGCTGCA GAGTCTAATG 
     2221 CGACCTTTTT CAACATAAGT GCTGCCAGTT TAACTTCAAA ATATGTGGGA GAAGGAGAGA 
     2281 AATTGGTGAG AGCTCTCTTT GCTGTGGCTC GAGAACTTCA ACCATCTATA ATTTTTATAG 
     2341 ATGAAGTTGA CAGTCTTTTG TGTGAGAGAC GGGAAGGGGA GCACGACGCT AGCAGACGGC 
     2401 TAAAGACGGA ATTTTTAATA GAATTTGACG GGGTGCAATC TGCTGGAGAT GACAGAGTAC 
     2461 TTGTAATGGG TGCAACTAAC AGGCCCCAAG AGCTTGATGA AGCTGTTCTC AGGCGTTTCA 
     2521 TTAAACGGGT ATATGTGTCC TTACCAAATG AGGAGACAAG ACTCCTTCTG CTTAAAAACC 
     2581 TGTTGTGTAA ACAAGGAAGT CCACTGACCC AAAAAGAACT CGCACAGCTT GCTAGAATGA 
     2641 CCGATGGATA CTCTGGAAGT GATCTGACCG CTTTGGCCAA GGATGCAGCC CTGGGTCCTA 
     2701 TCCGAGAACT GAAGCCAGAG CAGGTGAAGA ATATGTCTGC CAGTGAGATG AGAAATATTC 
     2761 GATTATCTGA CTTCACAGAA TCCTTAAAAA AGATAAAACG CAGTGTGAGT CCTCAGACCT 
     2821 TAGAAGCATA CATACGCTGG AACAAGGATT TTGGAGACAC CACTGTTACG CGTACGCGGC 
     2881 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     2941 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3001 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3061 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3121 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3181 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3241 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3301 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3361 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3421 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3481 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     3541 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     3601 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     3661 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     3721 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     3781 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     3841 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     3901 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     3961 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4021 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4081 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4141 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4201 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4261 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4321 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4381 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4441 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4501 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     4561 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     4621 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     4681 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     4741 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     4801 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     4861 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     4921 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     4981 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5041 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5101 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5161 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5221 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5281 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5341 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5401 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5461 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     5521 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     5581 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     5641 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     5701 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     5761 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     5821 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     5881 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     5941 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6001 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6061 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6121 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6181 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6241 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6301 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6361 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6421 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6481 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     6541 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     6601 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     6661 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//