LOCUS       MR209397                6714 bp    DNA     circular  SYN 
DEFINITION  Podn (Myc-DDK-tagged) - Mouse podocan (Podn)
ACCESSION   NM_172874
VERSION     NM_172874.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2871..2962 
                     /label="Myc-DDK"  
     misc_feature    2992..3577 
                     /label="PolyA signal"  
     misc_feature    3049..3068 
                     /label="Restriction Enzyme"  
     misc_feature    3853..4525 
                     /label="ColE1"  
     misc_feature    4955..5749 
                     /label="Kan/Neo"  
     misc_feature    5784..6086 
                     /label="SV40 ori"  
     misc_feature    6172..6649 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2853..2859 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2853 
                     /label="Podn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGCAGC AGGGGCCTGC CACTCCTACT GCTGGTGCTT CAGCTCTTCC 
     1081 TGGGCCCTGT GCTGCCTGTG AGGGCACCTG TGTTTGGCCG AAGTGACACC CCCACCCTGA 
     1141 GCCCCGAGGA GAATGAATTT GTGGAGGAAG AGAATCAGCC AGTGCTGGTT CTGAGCTCCG 
     1201 AGGAGCCAGA GCCTGGCCCA GCCACTGTCG ACTGTCCCCG AGATTGTGCC TGTTCCCAGG 
     1261 AAGGTGTAGT GGACTGTGGT GGCATTGACC TGCGTGAGTT TCCAGGCGAC CTGCCCGAGC 
     1321 ACACCAACCA TCTCTCCTTG CAGAACAACC AGCTGGAGAA GATCTACCCC GAGGAGCTGT 
     1381 CCCGGCTGCA GCGGCTGGAG ACGCTGAACC TGCAGAACAA CCGCCTGACA TCCCGAGGGC 
     1441 TCCCAGAGGA GGCATTTGAG CATCTTACTA GCCTCAATTA CCTGTACCTG GCCAACAACA 
     1501 AGCTGACACT GGCACCCCGA TTCCTGCCAA ACGCCCTGAT CAGTGTGGAC TTTGCTGCCA 
     1561 ATTATCTCAC TAAGATCTAT GGACTCACCT TTGGCCAAAA GCCAAATCTG AGGTCTGTGT 
     1621 ACCTGCATAA CAACAAGCTA GCAGATGCCG GGCTGCCGGA CCACATGTTC AATGGCTCCA 
     1681 GCAACGTCGA GATCCTAATC CTGTCCAGCA ACTTCCTGCG CCACGTGCCC AAGCACCTGC 
     1741 CACCCGCTCT GTACAAGCTG CACCTCAAGA ACAATAAGCT AGAGAAGATC CCCCCTGGGG 
     1801 CCTTCAGTGA GCTGAGCAAC CTACGGGAAC TCTACCTGCA GAACAACTAC CTGACCGACG 
     1861 AGGGTCTGGA CAACGAGACC TTCTGGAAGC TGTCCAGCCT GGAGTACCTG GACTTGTCCA 
     1921 GCAACAACCT GTCGAGGGTC CCAGCGGGTC TTCCCCGCAG CCTGGTCCTG CTGCACCTGG 
     1981 AGAAAAATGC CATCCAGAGC GTAGAAGCTG ATGTGCTGAC ACCCATCCGC AACCTGGAGT 
     2041 ACCTGCTGCT ACATAGCAAC CAGCTGCAGG CCAAGGGTAT CCACCCACTG GCCTTCCAGG 
     2101 GCCTCAAGAA GCTCCACACA GTGCATCTAT ACAACAACGC GCTGGAACGT GTGCCCAGCG 
     2161 GCCTGCCCCG CCGAGTGCGC ACCCTCATGA TCCTGCACAA CCAGATTACA GGCATAGGCC 
     2221 GTGAGGACTT CGCTACCACC TACTTCCTGG AAGAGCTCAA CCTCAGCTAC AACCGCATCA 
     2281 CCAGCCCACA GATGCACCGA GATGCCTTCC GCAAGCTACG CCTGCTGCGT TCACTTGACT 
     2341 TGTCTGGCAA CCGTCTGCAA ACACTGCCTC CAGGCCTGCC GAAAAACGTA CACGTGCTCA 
     2401 AGGTCAAGCG GAATGAGCTG GCTGCCCTGG CACGTGGGGC ACTAGCTGGC ATGGCCCAGC 
     2461 TTCGGGAACT CTACCTCACA GGCAACCGAC TGCGAAGCCG GGCCCTGGGA CCCCGTGCCT 
     2521 GGGTGGACCT TGCTGGTCTG CAGCTGCTGG ACATCGCTGG GAATCAGCTC ACAGAGGTCC 
     2581 CTGAGGGGCT CCCCCCATCT CTGGAGTATC TGTACCTGCA GAATAACAAG ATTAGTGCCG 
     2641 TTCCTGCCAA CGCCTTTGAC TCCACTCCCA ACCTTAAGGG GATCTTTCTC AGGTTCAACA 
     2701 AGCTGGCTGT GGGCTCCGTG GTGGAAAGCG CCTTCCGGAG GCTGAAACAC CTGCAGGTCT 
     2761 TGGACATTGA AGGCAACTTT GAGTTTGGTA ATGGTTCCAA GGACAAAGAT GAGGAAGAGG 
     2821 AAGAAGAGGA GGAAGAGGAA GATGAGGAAG AGGAAACTAG AACGCGTACG CGGCCGCTCG 
     2881 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     2941 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3001 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3061 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3121 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3181 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3241 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3301 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3361 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3421 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3481 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3541 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3601 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3661 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3721 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3781 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     3841 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     3901 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     3961 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4021 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4081 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4141 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4201 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4261 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4321 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4381 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4441 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4501 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4561 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4621 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4681 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4741 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4801 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     4861 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     4921 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     4981 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5041 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5101 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5161 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5221 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5281 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5341 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5401 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5461 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5521 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5581 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5641 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5701 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5761 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     5821 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     5881 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     5941 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6001 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6061 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6121 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6181 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6241 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6301 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6361 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6421 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6481 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6541 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6601 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6661 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//