LOCUS       MR209348                6696 bp    DNA     circular  SYN 
DEFINITION  Afp (Myc-DDK-tagged) - Mouse alpha fetoprotein (Afp)
ACCESSION   NM_007423
VERSION     NM_007423.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2853..2944 
                     /label="Myc-DDK"  
     misc_feature    2974..3559 
                     /label="PolyA signal"  
     misc_feature    3031..3050 
                     /label="Restriction Enzyme"  
     misc_feature    3835..4507 
                     /label="ColE1"  
     misc_feature    4937..5731 
                     /label="Kan/Neo"  
     misc_feature    5766..6068 
                     /label="SV40 ori"  
     misc_feature    6154..6631 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2835..2841 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2835 
                     /label="Afp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTGGATC ACACCCGCTT CCCTCATCCT CCTGCTACAT TTCGCTGCGT 
     1081 CCAAAGCATT GCACGAAAAT GAGTTTGGGA TAGCTTCCAC GTTAGATTCC TCCCAGTGCG 
     1141 TGACGGAGAA GAATGTGCTT AGCATAGCTA CCATCACCTT TACCCAGTTT GTTCCGGAAG 
     1201 CCACCGAGGA GGAAGTGAAC AAAATGACTA GCGATGTGTT GGCTGCAATG AAGAAAAACT 
     1261 CTGGCGATGG GTGTTTAGAA AGCCAGCTAT CTGTGTTTCT GGATGAAATT TGTCATGAGA 
     1321 CGGAACTCTC TAACAAGTAT GGACTCTCAG GCTGCTGCAG CCAAAGTGGA GTGGAAAGAC 
     1381 ATCAGTGTCT GCTGGCACGC AAGAAGACTG CTCCGGCCTC TGTCCCACCC TTCCAGTTTC 
     1441 CAGAACCTGC CGAGAGTTGC AAAGCACATG AAGAAAACAG GGCAGTGTTC ATGAACAGGT 
     1501 TCATCTATGA AGTGTCAAGG AGGAACCCCT TCATGTATGC CCCAGCCATT CTGTCCTTGG 
     1561 CTGCTCAGTA CGACAAGGTC GTTCTGGCAT GCTGCAAAGC TGACAACAAG GAGGAGTGCT 
     1621 TCCAGACAAA GAGAGCATCC ATTGCAAAGG AATTAAGAGA AGGAAGCATG TTAAATGAGC 
     1681 ATGTATGTTC AGTGATAAGA AAATTTGGAT CCCGAAACCT CCAGGCAACA ACCATTATTA 
     1741 AGCTAAGTCA AAAGTTAACT GAAGCAAATT TTACTGAGAT TCAGAAGCTG GCCCTGGATG 
     1801 TGGCTCACAT CCACGAGGAG TGTTGCCAAG GAAACTCGCT GGAGTGTCTG CAGGATGGGG 
     1861 AAAAAGTCAT GACATATATA TGTTCTCAAC AAAATATTCT GTCAAGCAAA ATAGCAGAGT 
     1921 GCTGCAAATT ACCCATGATC CAACTAGGCT TCTGCATAAT TCACGCAGAG AATGGCGTCA 
     1981 AACCTGAAGG CTTATCTCTA AATCCAAGCC AGTTTTTGGG AGACAGAAAT TTTGCCCAAT 
     2041 TTTCTTCAGA GGAAAAAATC ATGTTCATGG CAAGCTTTCT TCATGAATAC TCAAGAACTC 
     2101 ACCCCAACCT TCCTGTCTCA GTCATTCTAA GAATTGCTAA AACGTACCAG GAAATATTGG 
     2161 AGAAGTGTTC CCAGTCTGGA AATCTACCTG GATGTCAGGA CAATCTGGAA GAAGAATTGC 
     2221 AGAAACACAT CGAGGAGAGC CAGGCACTGT CCAAGCAAAG CTGCGCTCTC TACCAGACCT 
     2281 TAGGAGACTA CAAATTACAA AATCTGTTCC TTATTGGTTA CACGAGGAAA GCCCCTCAGC 
     2341 TGACCTCAGC AGAGCTGATC GACCTCACCG GGAAGATGGT GAGCATTGCC TCCACGTGCT 
     2401 GCCAGCTCAG CGAGGAGAAA TGGTCCGGCT GTGGTGAGGG AATGGCCGAC ATTTTCATTG 
     2461 GACATTTGTG TATAAGGAAT GAAGCAAGCC CTGTGAACTC TGGTATCAGC CACTGCTGCA 
     2521 ACTCTTCGTA TTCCAACAGG AGGCTATGCA TCACCAGTTT TCTGAGGGAT GAAACCTATG 
     2581 CCCCTCCCCC ATTCTCTGAG GATAAATTCA TCTTCCACAA GGATCTGTGC CAAGCTCAGG 
     2641 GCAAAGCCCT ACAGACCATG AAACAAGAGC TTCTCATTAA CCTGGTGAAG CAAAAGCCTG 
     2701 AACTGACAGA GGAGCAGCTG GCGGCTGTCA CTGCAGATTT CTCGGGCCTT TTGGAGAAGT 
     2761 GCTGCAAAGC CCAGGACCAG GAAGTCTGTT TCACAGAAGA GGGTCCAAAG TTGATTTCCA 
     2821 AAACTCGTGA TGCTTTGGGC GTTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     2881 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     2941 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3001 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3061 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3121 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3181 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3241 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3301 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3361 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3421 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3481 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3541 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3601 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3661 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3721 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3781 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     3841 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     3901 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     3961 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4021 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4081 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4141 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4201 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4261 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4321 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4381 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4441 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4501 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4561 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4621 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4681 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4741 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4801 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     4861 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     4921 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     4981 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5041 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5101 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5161 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5221 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5281 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5341 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5401 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5461 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5521 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5581 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5641 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5701 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5761 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     5821 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     5881 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     5941 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6001 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6061 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6121 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6181 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6241 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6301 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6361 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6421 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6481 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6541 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6601 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6661 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//