LOCUS       MR209300                6678 bp    DNA     circular  SYN 
DEFINITION  Cel (Myc-DDK-tagged) - Mouse carboxyl ester lipase (Cel)
ACCESSION   NM_009885
VERSION     NM_009885.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2835..2926 
                     /label="Myc-DDK"  
     misc_feature    2956..3541 
                     /label="PolyA signal"  
     misc_feature    3013..3032 
                     /label="Restriction Enzyme"  
     misc_feature    3817..4489 
                     /label="ColE1"  
     misc_feature    4919..5713 
                     /label="Kan/Neo"  
     misc_feature    5748..6050 
                     /label="SV40 ori"  
     misc_feature    6136..6613 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2817..2823 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2817 
                     /label="Cel"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCGCCTG GAGGTTCTAT TTCTTGGCCT CACCTGCTGC TTGGCAGCGG 
     1081 CTTGTGCTGC AAAGTTGGGG GCTGTGTACA CAGAAGGCGG TTTCGTGGAG GGCGTCAACA 
     1141 AGAAGCTCAG TCTCTTGGGT GGTGACTCTG TTGACATCTT CAAGGGTATC CCCTTTGCCA 
     1201 CTGCCAAGAC CTTGGAGAAT CCTCAGCGTC ACCCTGGCTG GCAAGGGACG CTGAAGGCTA 
     1261 CAAACTTCAA GAAACGATGC CTACAGGCCA CCATCACCCA GGACAACACC TATGGGCAAG 
     1321 AAGACTGCCT CTACCTCAAT ATCTGGGTGC CCCAGGGCAG GAAGCAGGTG TCCCACAACC 
     1381 TGCCTGTGAT GGTCTGGATC TATGGAGGTG CCTTCCTCAT GGGATCTGGC CAAGGGGCCA 
     1441 ATTTTCTCAA AAACTACCTG TATGACGGGG AGGAGATCGC CACTCGGGGC AACGTCATTG 
     1501 TGGTCACCTT CAACTACCGT GTTGGACCCT TGGGTTTCCT TAGCACCGGG GATGCTAACC 
     1561 TTCCAGGTAA CTTTGGTCTT CGAGATCAGC ACATGGCTAT CGCCTGGGTG AAGAGGAACA 
     1621 TTGCAGCCTT TGGGGGGGAC CCTGATAACA TCACCATCTT TGGGGAATCT GCTGGAGCTG 
     1681 CCAGTGTCTC CCTGCAGACC CTCTCCCCAT ACAACAAGGG TCTCATCCGG AGAGCCATCA 
     1741 GTCAGAGTGG TATGGCACTG AGCCCCTGGG CCATCCAGAA GAACCCACTT TTCTGGGCCA 
     1801 AAACGATTGC TAAGAAGGTA GGATGCCCCA CAGAGGATAC TGGCAAGATG GCTGCGTGTC 
     1861 TGAAGATCAC AGATCCCCGG GCCTTGACAC TGGCCTACAA GTTGCCCGTG AAAAAGCAGG 
     1921 AGTACCCTGT TGTGCACTAC CTGGCCTTCA TCCCTGTCAT CGATGGTGAC TTCATTCCCG 
     1981 ATGATCCCAT CAACCTATAC AACAACACTG CTGACATTGA CTACATAGCG GGCATTAACA 
     2041 ACATGGACGG CCACCTATTT GCTACCATTG ATGTGCCCGC TGTCGACAAG ACCAAGCAGA 
     2101 CTGTCACCGA GGAGGACTTC TACAGGCTAG TCAGTGGACA CACTGTCGCC AAGGGGCTGA 
     2161 AGGGGGCCCA AGCCACCTTT GACATCTACA CTGAGTCCTG GGCCCAGGAC CCGTCCCAGG 
     2221 AGAACATGAA GAAGACAATG GTGGCCTTTG AGACTGACGT ACTCTTCCTG ATCCCCACGG 
     2281 AGATGGCGCT GGCCCAGCAC AAAGCCCATG CCAAGAGCGC CAAGACCTAT TCTTACCTGT 
     2341 TTTCCCACCC TTCACGGATG CCTATCTACC CCAAATGGAT GGGGGCAGAC CATGCTGATG 
     2401 ACCTCCAGTA CGTCTTTGGA AAGCCTTTTG CCACCCCACT GGGCTACCGG CCCCAAGATA 
     2461 GGGCTGTCTC CAAGGCCATG ATCGCCTATT GGACCAACTT TGCCAGGAGT GGGGACCCCA 
     2521 ACATGGGCAA CTCACCTGTG CCCACACACT GGTACCCTTA TACCTTGGAG AATGGCAACT 
     2581 ACCTGGACAT CACTAAGACG ATAACCAGCG CTTCCATGAA GGAGCACCTG AGAGAAAAGT 
     2641 TCCTGAAGTT CTGGGCTGTG ACATTCGAGG TGCTGCCCAC AGTGACTGGT GACCAGGACA 
     2701 CCCTCACTCC CCCTGAGGAT GACTCAGAGG TTGCCCCTGA CCCACCTTCA GACGACTCCC 
     2761 AGGTTGTTCC TGTCCCACCT ACAGATGACT CTGTGGAGGC TCAGATGCCT GCCACCATTG 
     2821 GCTTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     2881 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     2941 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3001 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3061 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3121 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3181 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3241 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3301 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3361 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3421 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3481 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3541 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3601 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3661 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     3721 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     3781 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     3841 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     3901 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     3961 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4021 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4081 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4141 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4201 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4261 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4321 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4381 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4441 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4501 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4561 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4621 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4681 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     4741 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     4801 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     4861 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     4921 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     4981 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5041 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5101 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5161 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5221 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5281 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5341 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5401 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5461 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5521 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5581 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5641 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     5701 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     5761 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     5821 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     5881 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     5941 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6001 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6061 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6121 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6181 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6241 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6301 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6361 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6421 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6481 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6541 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6601 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6661 AAATTTAACG CGAATTTT
//