LOCUS       MR209166L3                8816 bp    DNA     circular  SYN 
DEFINITION  Gspt1 (Myc-DDK-tagged) - Mouse G1 to S phase transition 1 (Gspt1), transcript variant 1
ACCESSION   NM_146066
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7107..7287 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8015..8674 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7335..7954 
                     /label="ColE1"  
     misc_feature    5147..5170 
                     /label="DDK"  
     misc_feature    5982..6002 
                     /label="LR50"  
     misc_feature    5099..5170 
                     /label="MYC"  
     misc_feature    5180..5245 
                     /label="P2A"  
     misc_feature    5246..5845 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    5991..6582 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5081..5087 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5081 
                     /label="Gspt1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCGCGGTG GCCGAGGCCC AGCGTGAGAA 
     3361 CCTCAGCGCG GCTTTCAGCC GGCAACTCAA CGTCAACGCC AAGCCTTTCG TCCCCAACGT 
     3421 CCACGCCGCT GAATTCGTGC CGTCCTTCCT GCGGGGTCCG GCCCAGCCGC CGCTATCCCC 
     3481 AGCTGGCGCA GCCGGCGGCG ACCACGGAGC AGGCAGCGGC GCGGGAGGCC CTTCGGAACC 
     3541 TGTAGAGTCC TCTCAAGACC AGTCGTGTGA AGGTTCAAAT TCTACTGTTA GCATGGAACT 
     3601 TTCAGAACCT GTTGTGGAAA ATGGAGAGAC AGAAATGTCC CCAGAAGAAT CATGGGAGCA 
     3661 CAAAGAAGAA ATAAGTGAAG CGGAGCCAGG GGGTGGTTCC TCAGGAGATG GAAGGCCACC 
     3721 AGAGGAAAGC ACCCAAGAAA TGATGGAGGA GGAGGAAGAA ATACCGAAAC CTAAGTCAGC 
     3781 GGTGGCACCA CCAGGTGCTC CCAAGAAGGA ACATGTAAAT GTAGTGTTCA TCGGACATGT 
     3841 TGATGCAGGC AAGTCAACCA TTGGAGGACA AATAATGTAT TTGACTGGAA TGGTCGACAA 
     3901 AAGGACACTT GAGAAGTATG AACGAGAAGC TAAAGAAAAA AACAGAGAAA CTTGGTACTT 
     3961 GTCTTGGGCC TTAGACACGA ATCAGGAAGA ACGAGACAAG GGCAAAACCG TGGAGGTGGG 
     4021 TCGTGCCTAT TTTGAAACGG AAAAGAAGCA TTTTACAATT CTAGATGCTC CTGGCCATAA 
     4081 GAGTTTTGTC CCAAATATGA TTGGTGGTGC CTCTCAAGCT GACTTGGCTG TACTGGTAAT 
     4141 CTCAGCCAGG AAAGGAGAGT TTGAAACTGG ATTTGAAAAA GGAGGACAGA CAAGAGAACA 
     4201 TGCAATGTTG GCAAAGACAG CAGGTGTCAA ACACTTAATT GTGCTTATTA ATAAGATGGA 
     4261 TGATCCAACG GTAAACTGGA GCAACGAGAG ATATGAAGAA TGCAAAGAGA AACTAGTACC 
     4321 ATTTTTGAAA AAAGTTGGCT TCAATCCCAA AAAGGACATT CATTTTATGC CGTGCTCAGG 
     4381 ACTGACAGGA GCGAATCTTA AAGAACAATC AGATTTCTGT CCTTGGTACA TTGGCTTACC 
     4441 ATTTATTCCA TATCTGGATA ATTTGCCAAA CTTCAATAGA TCAGTTGATG GACCAATCAG 
     4501 GCTGCCAATT GTCGATAAGT ACAAGGATAT GGGGACTGTG GTCCTGGGAA AGCTGGAATC 
     4561 TGGATCTATT TGTAAAGGCC AGCAGCTTGT AATGATGCCA AATAAGCACA ACGTGGAAGT 
     4621 TCTTGGAATA CTTTCTGATG ATGTAGAAAC GGACTCTGTA GCTCCAGGTG AAAACCTCAA 
     4681 AATCAGACTG AAAGGAATTG AAGAGGAAGA GATTCTTCCA GGATTCATCC TTTGTGATCT 
     4741 TAATAATCTT TGTCATTCTG GACGCACATT TGATGCTCAG ATAGTGATTA TAGAGCACAA 
     4801 GTCCATCATC TGCCCAGGGT ATAATGCGGT GCTGCATATT CATACCTGTA TCGAAGAAGT 
     4861 CGAAATAACA GCCTTAATCT GCTTGGTAGA CAAGAAATCA GGAGAGAAAA GTAAGACTCG 
     4921 ACCCCGTTTT GTAAAACAAG ATCAAGTATG CATTGCGCGT TTAAGGACAG CAGGAACCAT 
     4981 CTGCCTGGAG ACCTTTAAGG ACTTCCCTCA GATGGGACGT TTTACCTTAA GAGATGAGGG 
     5041 TAAGACCATT GCAATTGGAA AAGTTCTGAA ACTGGTTCCA GAGAAAGACA CGCGTACGCG 
     5101 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     5161 CAAGGATGAC GACGATAAGG TTTGGGTAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA 
     5221 GCAGGCTGGA GACGTGGAGG AGAACCCTGG ACCTATGACC GAGTACAAGC CCACGGTGCG 
     5281 CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 
     5341 CTACCCCGCC ACGCGCCACA CCGTCGATCC GGATCGCCAC ATCGAGCGGG TCACCGAGCT 
     5401 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 
     5461 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 
     5521 CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 
     5581 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 
     5641 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 
     5701 GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 
     5761 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 
     5821 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATGTACA CCCAAACGGC CGGCCGCGGT 
     5881 CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG 
     5941 TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA 
     6001 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 
     6061 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 
     6121 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 
     6181 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 
     6241 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 
     6301 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 
     6361 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG 
     6421 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 
     6481 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 
     6541 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC 
     6601 GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC 
     6661 AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA 
     6721 CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA 
     6781 AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT 
     6841 CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA 
     6901 GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG 
     6961 AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT 
     7021 GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT 
     7081 CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC 
     7141 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 
     7201 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 
     7261 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA 
     7321 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 
     7381 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 
     7441 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 
     7501 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 
     7561 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 
     7621 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 
     7681 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 
     7741 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 
     7801 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 
     7861 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 
     7921 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 
     7981 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC 
     8041 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA 
     8101 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT 
     8161 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC 
     8221 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG 
     8281 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT 
     8341 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA 
     8401 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA 
     8461 TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA 
     8521 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT 
     8581 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT 
     8641 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT 
     8701 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8761 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//