LOCUS       MR209135                6633 bp    DNA     circular  SYN 
DEFINITION  Klhdc4 (Myc-DDK-tagged) - Mouse kelch domain containing 4 (Klhdc4)
ACCESSION   NM_145605
VERSION     NM_145605.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2790..2881 
                     /label="Myc-DDK"  
     misc_feature    2911..3496 
                     /label="PolyA signal"  
     misc_feature    2968..2987 
                     /label="Restriction Enzyme"  
     misc_feature    3772..4444 
                     /label="ColE1"  
     misc_feature    4874..5668 
                     /label="Kan/Neo"  
     misc_feature    5703..6005 
                     /label="SV40 ori"  
     misc_feature    6091..6568 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2772..2778 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2772 
                     /label="Klhdc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAAGAAG GGAAAGAAGG AGAAGAAGGG CCGCGGCGCG GAGAAGACGG 
     1081 CCGCCAAGAT GGAGAAGAAG GTGTCCAAGC GCTCGCGGAA GGAGGAGGAA GACCTGGAGG 
     1141 CCCTCATAGC CCATTTCCAG ACCTTGGATG CCAAAAAGAC CCAGGTCACA GAAACACCAT 
     1201 GTCCTCCACC CTCCCCACGG TTAAACGCCT CCCTGTCTGC TCATCCTGAG AAAGATGAGC 
     1261 TGATCCTTTT CGGAGGTGAA TATTTCAATG GCCAAAAAAC CTTCATGTAT AATGAACTCT 
     1321 ACATCTACAG TATCAGAAAG GACACCTGGA CAAAAGTTGA CATCCCTGGC CCACCTCCCA 
     1381 GGCGCTGTGC TCATCAGGCT GTGGTGGTAC CCCAGGGTGG TGGGCAGCTG TGGGTCTTCG 
     1441 GTGGGGAGTT TGCATCTCCC GATGGCGAGC AGTTCTACCA TTACAAGGAC CTCTGGGTCC 
     1501 TGCACTTGGC CACCAAGACC TGGGAACAGA TTCGATCAAC AGGGGGTCCT TCAGGTCGAA 
     1561 GCGGGCATCG GATGGTGGCC TGGAAGCGAC AGCTAATTCT TTTTGGTGGC TTCCATGAAA 
     1621 GTGCAAGGGA CTACATCTAC TACAGTGATG TGTACACCTT CAGCCTGGAC ACCTTCCAGT 
     1681 GGAGCAAGCT GTCCCCATCA GGCCCCGGGC CCACGCCCAG GTCAGGCTGC CTGATGGCTG 
     1741 TCACTCCCCA GGGCAGCATA GCCATCTATG GAGGTTACTC AAAGCAGAGA GTCAAGAAAG 
     1801 ATGTGGACAA AGGCACCCAG CACTCAGACA TGTTCCTGCT GAAGCCTGCG GAAGGGGGAG 
     1861 AAGGCAAATG GGCTTGGACC AGGATTAACC CTTCGGGGGT CAAGCCTACT GCAAGGTCTG 
     1921 GCTTTTCTGT GGCTGTGGCT CCAAATCATC AGATACTGGT CTTCGGGGGC GTGTGTGATG 
     1981 AGGAAGAGGA GGAGAGCCTG GAGGGCTCCT TCTTCAGTGA CCTGTATATC TATGATTCGG 
     2041 CCAAGAGTCG CTGGTTTGCA GCACAGCTGA AGGGCCCCAA GTCTGAGAAG AAGAAACGGA 
     2101 GGCGGGGCAA GGCAGAGGAT CCTGAAGGCA CCACTGAACA GGAGACTGGG GGGAGCAGTG 
     2161 CCCCAGAGCC ACTGGAGGTG ATAAAAGAGG TGGTGTCTGA AGACGGGACA GTGGTCACCA 
     2221 TTAAGCAAGT ACTCACTCCC TCAGGGTTGG GGGTGCAGCC CTCACCTAAG GCTGATGACA 
     2281 GTGCCTCAGA GGCCAGCAGC ACTGGACAGG AGCCATGCCC TCGTTCCAAC GCCATGCTGG 
     2341 CTGTCAAGCA TGGACTGCTG TACGTCTATG GCGGCATGTT TGAAGCTGGT GACCGCCAGG 
     2401 TGACCCTTAG TGACCTCTAC TGCCTTGACC TTCACAAGAT GGAAGAGTGG AAAACCTTGG 
     2461 TGGAGATGGA TCCGAAATCC CAGGAGTGGC TGGAGGAGTC GGACTCAGAA GAGGACAGCA 
     2521 GCTCAGATGA AGAGAGTGCA GATGGAGAGG ATGAAGACCA GGAGGACAGT GCTGAGGAGG 
     2581 GAGCAGATCC GCAGCACCCA GAGGTGGCCC GTGGGGAGCA ATTCGAAGAG TACCTGTCCC 
     2641 GGACTGAGCA GCACTGGCTG AAGCTGGCCC GGAGCCATGT GGGGCCTGAA GCAAAGGAGA 
     2701 AGAAAGTGTT GAAAGTAGCC CAGGCCATGG CAAAATCATG CTTTGATGAT GCAGTTCAAG 
     2761 ACATTGCACA GGCCAGACAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     2821 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     2881 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     2941 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3001 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3061 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3121 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3181 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3241 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3301 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3361 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3421 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3481 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3541 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3601 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3661 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3721 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     3781 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     3841 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     3901 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     3961 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4021 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4081 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4141 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4201 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4261 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4321 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4381 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4441 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4501 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4561 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4621 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4681 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4741 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     4801 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     4861 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     4921 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     4981 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5041 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5101 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5161 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5221 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5281 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5341 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5401 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5461 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5521 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5581 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5641 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5701 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     5761 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     5821 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     5881 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     5941 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6001 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6061 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6121 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6181 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6241 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6301 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6361 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6421 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6481 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6541 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6601 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//