LOCUS       MR209004                6597 bp    DNA     circular  SYN 
DEFINITION  Sqle (Myc-DDK-tagged) - Mouse squalene epoxidase (Sqle)
ACCESSION   NM_009270
VERSION     NM_009270.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2754..2845 
                     /label="Myc-DDK"  
     misc_feature    2875..3460 
                     /label="PolyA signal"  
     misc_feature    2932..2951 
                     /label="Restriction Enzyme"  
     misc_feature    3736..4408 
                     /label="ColE1"  
     misc_feature    4838..5632 
                     /label="Kan/Neo"  
     misc_feature    5667..5969 
                     /label="SV40 ori"  
     misc_feature    6055..6532 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2736..2742 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2736 
                     /label="Sqle"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGACTTTT CTTGGCATTG CTACCTTCAC CTATTTTTAT AAGAAATGCG 
     1081 GGGATGTCAC CCTGGCCAAC AAGGAGCTCC TGCTGTGCGT GCTGGTGTTC CTGTCGCTGG 
     1141 GCCTGGTGCT CTCCTACCGC TGTCGCCATC GACACGGGGG CCTCCTTGGC CGCCATCAGA 
     1201 GCGGCGCCCA GTTCGCTGCC TTCTCGGATA TTCTCTCTGC TTTGCCTCTT ATTGGCTTCT 
     1261 TCTGGGCCAA GTCACCTGAA TCAGAAAAGA AAGAACAGCT GGAGTCCAAG AAGTGCAGAA 
     1321 AAGAAATCGG TCTTTCAGAA ACGACATTAA CAGGAGCAGC TACCTCAGTG TCGACCTCGT 
     1381 TCGTGACGGA CCCGGAAGTG ATCATCGTGG GATCTGGTGT GCTTGGATCT GCCTTGGCAG 
     1441 CAGTTCTCTC CAGAGATGGA AGAAAGGTGA CGGTCATCGA GAGAGATTTA AAAGAGCCCG 
     1501 ACAGGATAGT TGGGGAGCTG CTGCAGCCAG GAGGCTACCG TGTTCTCCAG GAGCTGGGCC 
     1561 TTGGAGATAC AGTAGAAGGT CTCAATGCCC ATCATATACA TGGCTACATA GTTCATGACT 
     1621 ATGAAAGCAG ATCTGAAGTT CAAATTCCGT ACCCACTGTC AGAAACCAAC CAAGTGCAGA 
     1681 GTGGAATTGC TTTTCACCAT GGCCGATTCA TCATGAGTCT CCGGAAAGCA GCTATGGCAG 
     1741 AGCCCAATGT AAAGTTTATA GAAGGTGTTG TGCTTCAGTT ACTAGAGGAA GATGATGCTG 
     1801 TAATCGGCGT GCAATACAAG GACAAGGAGA CTGGGGACAC CAAGGAGCTC CACGCCCCGC 
     1861 TCACCGTTGT TGCGGATGGA CTCTTCTCCA AGTTCAGGAA GAGCCTCATC TCCAGTAAAG 
     1921 TCTCCGTTTC TTCCCACTTC GTTGGCTTCC TTATGAAGGA TGCACCACAG TTTAAACCCA 
     1981 ATTTTGCGGA GCTTGTTCTG GTCAACCCCA GTCCAGTTCT CATCTACCAG ATTTCTTCCA 
     2041 GTGAAACTAG GGTACTTGTT GATATTCGGG GAGAATTGCC AAGAAACCTA AGAGAATACA 
     2101 TGGCTGAACA AATTTACCCA CAGTTACCTG AGCACCTGAA GGAGTCATTT CTGGAGGCCT 
     2161 CTCAGAATGG TCGTCTGCGG ACCATGCCGG CCAGCTTCCT TCCTCCTTCC TCAGTGAACA 
     2221 AACGAGGCGT CCTTATTCTG GGAGATGCGT ATAACCTGAG GCACCCTCTT ACTGGTGGAG 
     2281 GAATGACAGT TGCTTTAAAA GATATAAAAT TGTGGAGACA ACTGTTAAAA GACATTCCTG 
     2341 ACCTTTATGA TGATGCTGCT ATTTTCCAGG CCAAAAAATC ATTCTTTTGG TCAAGAAAAA 
     2401 GGACCCATTC CTTTGTTGTG AATGTATTGG CTCAGGCCTT GTATGAATTA TTTTCAGCTA 
     2461 CAGATGATTC CTTGCATCAG CTCCGAAAAG CTTGCTTTCT GTATTTTAAA CTTGGTGGAG 
     2521 AGTGTGTGAC CGGTCCTGTT GGGTTGCTTT CAATATTGTC TCCTCACCCT CTGGTGTTGA 
     2581 TTCGACACTT CTTTTCCGTT GCAATCTACG CCACGTATTT CTGCTTCAAA TCAGAGCCGT 
     2641 GGGCTACAAA ACCACGGGCC CTTTTCAGTA GCGGTGCTGT ACTGTACAAA GCGTGTTCTA 
     2701 TCCTATTTCC ACTCATCTAT TCAGAAATGA AGTATCTGGT TCATACGCGT ACGCGGCCGC 
     2761 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     2821 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     2881 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     2941 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3001 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3061 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3121 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3181 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3241 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3301 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3361 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3421 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3481 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3541 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     3601 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     3661 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     3721 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     3781 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     3841 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     3901 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     3961 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4021 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4081 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4141 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4201 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4261 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4321 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4381 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4441 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4501 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4561 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     4621 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     4681 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     4741 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     4801 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     4861 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     4921 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     4981 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5041 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5101 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5161 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5221 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5281 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5341 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5401 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5461 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5521 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     5581 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     5641 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     5701 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     5761 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     5821 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     5881 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     5941 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6001 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6061 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6121 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6181 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6241 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6301 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6361 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6421 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6481 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6541 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//