LOCUS       MR208726                6525 bp    DNA     circular  SYN 
DEFINITION  Sh2b3 (Myc-DDK-tagged) - Mouse SH2B adaptor protein 3 (Sh2b3)
ACCESSION   NM_008507
VERSION     NM_008507.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2682..2773 
                     /label="Myc-DDK"  
     misc_feature    2803..3388 
                     /label="PolyA signal"  
     misc_feature    2860..2879 
                     /label="Restriction Enzyme"  
     misc_feature    3664..4336 
                     /label="ColE1"  
     misc_feature    4766..5560 
                     /label="Kan/Neo"  
     misc_feature    5595..5897 
                     /label="SV40 ori"  
     misc_feature    5983..6460 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2664..2670 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2664 
                     /label="Sh2b3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACGAGCCC ACCGTGCAGC CGTCCCGCAC ATCCTCCGCA CCCGCCTCGC 
     1081 CGGCATCCCC ACGCGGCTGG AGCGACTTCT GCGAGCAGCA CGCAGCAGCG GCGGCCCGGG 
     1141 AGCTGGCCCG CCAGTACTGG TTGTTTGCGC GCGCGCACCC ACAGCCGCCG CGCGCGGACC 
     1201 TGGTGTCGCT GCAGTTCGCG GAGCTCTTCC AGCGCCACTT CTGCCGGGAG GTGCGCGAGA 
     1261 GCCTCGCAGG ACCGCCGGGT CACGACTACC GCGCCACTGC TCCGCCCCGC CCCGCGCTGC 
     1321 CCAAGGCACG CAGCTCCGAG GACCTGGGCC CGCGGCCCGC CTGTGCCCTG CAGCACCTGC 
     1381 GCCGCGGCCT GCGCCAGCTC TTCCGCCGCC GCTCGGCAGG GGAGCTGCCC GGGGCTACCA 
     1441 GTGACACCAA TGACATCGAC ACCACCGCAG CCAGCAGGCC GGGCCCGGCC CGCAAGTTGC 
     1501 TACCCTGGGG CCTGCGAGAG CCGCCCACTG AGGCGCTCAA GGAGGTCGTA TTGCGCTATA 
     1561 GCCTGGCGGA CGAGGCAGCA ATGGACAGCG GCGCACGCTG GCAGCGGGGT CGCCTGGTGC 
     1621 TTCGGTCTCC AGGTCCGGGC CACAGCCACT TTCTGCAGCT CTTCGATCCG CCCAAGAGCT 
     1681 CAAAGCCCAA GCTCCAAGAG GCCTGTTCCA GCATCCGGGA GGTCCGACCA TGTACACGCC 
     1741 TGGAGATGCC TGACAACCTC TACACCTTTG TGTTGAAGGT GCAGGACCAG ACAGACATCA 
     1801 TCTTTGAGGT GGGAGATGAA CAGCAGCTGA ACTCATGGCT GGCAGAGCTC AGGGCAAGCA 
     1861 CAGGCCTTGG GCTGGAGCAC CCGGACACCG AGTTACCTCT TTCCTTAGCG GCAGAGCCTG 
     1921 GCCCAGCTAG ATCCCCAAGG GGAAGCACTG ACTCCCTGGA CCAAGGTGCT TCACCTGGGG 
     1981 TGTTGCTGGA CCCAGCCTGC CAGAAAACAG ATCACTTCCT ATCCTGCTAC CCCTGGTTCC 
     2041 ACGGCCCCAT CTCCAGGGTG AGGGCTGCAC AGCTGGTCCA GCTCCAGGGC CCTGATGCCC 
     2101 ACGGCGTGTT CCTGGTGCGG CAGAGTGAGT CCCGGAGAGG AGAGTATGTA CTCACATTCA 
     2161 ACTTACAGGG CAGAGCCAAG CACTTACGCC TGGTGCTCAC AGAGCGTGGA CAGTGCCGGG 
     2221 TGCAACACCT GCACTTCCCC TCGGTGGTAG ATATGCTCCG CCACTTCCAG CGTTCTCCTA 
     2281 TCCCACTGGA ATGTGGAGCA GCTTGTGACG TCCGACTCTC TGGCTATGTG GTAGTCCTCT 
     2341 CTCAGGCACC AGGTTCCTCC AACACCGTCC TCTTCCCTTT TTCCCTTCCT CACTGGGATT 
     2401 CGGAGCTGGG TCATCCCCAC CTCAGCTCTG TTGGCTGTCC CCCCAGCCAT GGTGCAGAGG 
     2461 CTCTCCCTGG CCAAGTGACA CCACCTGAGC AGATCTTCCA CCTGGTGCCT TCTCCTGAGG 
     2521 AACTGGCCAA CAGTCTGCGG CAGCTGGAGC TCGAGTCTGT GAGCAGTGCC CGGGACTCGG 
     2581 ACTATGACAT GGACTCCTCT TCACGGGGCC ACCTTCGGGC CATTGACAAC CAGTACACCC 
     2641 CTCTCTCACA GCTGTGCAGA GAGGCAGACG TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     2701 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     2761 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     2821 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     2881 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     2941 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3001 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3061 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3121 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3181 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3241 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3301 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3361 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3421 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3481 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     3541 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     3601 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     3661 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     3721 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     3781 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     3841 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     3901 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     3961 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4021 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4081 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4141 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4201 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4261 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4321 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4381 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4441 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4501 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     4561 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     4621 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     4681 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     4741 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     4801 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     4861 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     4921 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     4981 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5041 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5101 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5161 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5221 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5281 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5341 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5401 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5461 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     5521 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     5581 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     5641 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     5701 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     5761 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     5821 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     5881 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     5941 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6001 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6061 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6121 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6181 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6241 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6301 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6361 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6421 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6481 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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