LOCUS       MR208301                6429 bp    DNA     circular  SYN 
DEFINITION  Dhcr24 (Myc-DDK-tagged) - Mouse 24-dehydrocholesterol reductase (Dhcr24)
ACCESSION   NM_053272
VERSION     NM_053272.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2586..2677 
                     /label="Myc-DDK"  
     misc_feature    2707..3292 
                     /label="PolyA signal"  
     misc_feature    2764..2783 
                     /label="Restriction Enzyme"  
     misc_feature    3568..4240 
                     /label="ColE1"  
     misc_feature    4670..5464 
                     /label="Kan/Neo"  
     misc_feature    5499..5801 
                     /label="SV40 ori"  
     misc_feature    5887..6364 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2568..2574 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2568 
                     /label="Dhcr24"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCGCC GTGTCGCTGG CCGTGTGCGC GCTGCTCTTT CTGCTCTGGG 
     1081 TGCGAGTGAA GGGGTTGGAG TTCGTTCTCA TCCACCAGCG CTGGGTGTTC GTGTGCCTCT 
     1141 TCTTGCTGCC GCTCTCGCTC ATCTTCGATA TCTACTACTA CGTGCGCGCC TGGGTGGTGT 
     1201 TCAAGCTGAG CAGTGCGCCG CGCCTGCACG AGCAGCGCGT GCGGGACATC CAGAAACAGG 
     1261 TCCGGGAATG GAAGGAACAG GGCAGTAAGA CCTTCATGTG CACGGGGCGC CCAGGCTGGC 
     1321 TCACTGTCTC GCTGCGAGTC GGAAAGTACA AGAAGACCCA TAAGAACATC ATGATCAACC 
     1381 TGATGGACAT CCTGGAGGTG GACACCAAGA AACAGATTGT TCGAGTGGAG CCCTTGGTGT 
     1441 CTATGGGTCA GGTGACAGCT TTGCTGAACT CCATTGGCTG GACCCTGCCT GTGTTGCCTG 
     1501 AGCTTGATGA CCTCACAGTG GGGGGCCTGA TCATGGGCAC AGGCATCGAG TCATCGTCCC 
     1561 ACAAGTATGG CCTGTTCCAA CACATTTGCA CTGCCTACGA GCTGATCCTG GCAGACGGCA 
     1621 GCTTTGTGCG CTGCACACCG TCTGAAAACT CAGACCTGTT CTATGCCGTG CCCTGGTCCT 
     1681 GTGGGACCCT GGGCTTCCTG GTGGCTGCCG AGATCCGGAT CATCCCGGCC AAGAAGTATG 
     1741 TCAAGCTGCG GTTTGAGCCT GTTCGGGGCC TGGAGGCCAT CTGTGAAAAA TTCACCCGCG 
     1801 AGTCCCAGCG GCTGGAGAAC CACTTCGTGG AAGGGTTGCT GTACTCCCTG GATGAGGCTG 
     1861 TCATCATGAC AGGGGTCATG ACGGACGACG TAGAGCCCAG CAAGCTGAAT AGCATTGGCA 
     1921 GTTACTACAA GCCCTGGTTC TTCAAGCATG TGGAGAACTA CCTGAAGACA AACCGGGAGG 
     1981 GCCTCGAATA CATTCCCCTG AGACACTACT ACCACCGACA CACGCGCAGC ATCTTCTGGG 
     2041 AGCTCCAGGA CATCATCCCT TTCGGCAACA ACCCCATCTT CCGCTACCTC TTCGGCTGGA 
     2101 TGGTGCCTCC CAAGATCTCC CTCCTGAAGC TGACCCAGGG CGAGACGCTA CGCAAGCTGT 
     2161 ACGAGCAGCA CCACGTGGTG CAGGACATGC TGGTGCCCAT GAAGTGCATG TCACAGGCCC 
     2221 TGCATACCTT CCAAAATGAC ATCCACGTCT ACCCCATCTG GCTGTGCCCA TTCATCCTGC 
     2281 CCAGCCAGCC AGGACTAGTG CATCCCAAGG GAGATGAAGC AGAGCTCTAC GTGGACATCG 
     2341 GGGCATACGG GGAGCCACGT GTGAAGCACT TCGAGGCCAG GTCCTGCATG AGGCAGCTGG 
     2401 AGAAGTTTGT GCGGAGTGTG CACGGGTTCC AAATGTTATA CGCCGATTGC TATATGAACC 
     2461 GCGAGGAATT CTGGGAGATG TTCGATGGCT CCTTGTACCA CAAGCTGCGC AAGCAGCTGG 
     2521 GCTGCCAGGA CGCCTTCCCT GAGGTGTACG ACAAGATCTG CAAGGCGGCA AGGCACACGC 
     2581 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     2641 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     2701 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     2761 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     2821 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     2881 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     2941 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3001 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3061 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3121 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3181 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3241 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3301 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3361 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     3421 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     3481 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     3541 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     3601 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     3661 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     3721 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     3781 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     3841 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     3901 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     3961 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4021 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4081 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4141 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4201 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4261 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4321 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4381 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     4441 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     4501 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     4561 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     4621 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     4681 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     4741 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     4801 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     4861 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     4921 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     4981 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5041 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5101 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5161 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5221 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5281 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5341 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     5401 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     5461 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     5521 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     5581 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     5641 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     5701 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     5761 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     5821 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     5881 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     5941 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6061 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6121 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6181 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6241 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6361 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     6421 GCGAATTTT
//