LOCUS       MR208194L4                9239 bp    DNA     circular  SYN 
DEFINITION  Sirpa (Myc-DDK-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 2
ACCESSION   NM_001177646
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7530..7710 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8438..9084 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6405..6425 
                     /label="LR50"  
     misc_feature    4868..5596 
                     /label="mGFP"  
     misc_feature    5603..5668 
                     /label="P2A"  
     misc_feature    7758..8377 
                     /label="pBR322 Ori"  
     misc_feature    5669..6268 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4847..4853 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4847 
                     /label="Sirpa"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGCCCGCC GGCCCGGCCC CTGGCCGCCT 
     3361 AGGGCCGCTG CTGCTCTGCC TGCTGCTCTC CGCGTCCTGT TTCTGTACAG GAGCCACGGG 
     3421 GAAGGAACTG AAGGTGACTC AGCCTGAGAA ATCAGTGTCT GTTGCTGCTG GGGATTCGAC 
     3481 CGTTCTGAAC TGCACTTTGA CCTCCTTGTT GCCGGTGGGA CCCATTAGGT GGTACAGAGG 
     3541 AGTAGGGCCA AGCCGGCTGT TGATCTACAG TTTCGCAGGA GAATACGTTC CTCGAATTAG 
     3601 AAATGTTTCA GATACTACTA AGAGAAACAA TATGGACTTT TCCATCCGTA TCAGTAATGT 
     3661 CACCCCAGCA GATGCTGGCA TCTACTACTG TGTGAAGTTC CAGAAAGGAT CATCAGAGCC 
     3721 TGACACAGAA ATACAATCTG GAGGGGGAAC AGAGGTCTAT GTACTCGCCA AACCTTCTCC 
     3781 ACCGGAGGTA TCCGGCCCAG CAGACAGGGG CATACCTGAC CAGAAAGTGA ACTTCACCTG 
     3841 CAAGTCTCAT GGCTTCTCTC CCCGGAATAT CACCCTGAAG TGGTTCAAAG ATGGGCAAGA 
     3901 ACTCCACCCC TTGGAGACCA CCGTGAACCC TAGTGGAAAG AATGTCTCCT ACAACATCTC 
     3961 CAGCACAGTC AGGGTGGTAC TAAACTCCAT GGATGTTAAT TCTAAGGTCA TCTGCGAGGT 
     4021 AGCCCACATC ACCTTGGATA GAAGCCCTCT TCGTGGGATT GCTAACCTGT CTAACTTCAT 
     4081 CCGAGTTTCA CCCACCGTGA AGGTCACCCA ACAGTCCCCG ACGTCAATGA ACCAGGTGAA 
     4141 CCTCACCTGC CGGGCTGAGA GGTTCTACCC CGAGGATCTC CAGCTGATCT GGCTGGAGAA 
     4201 TGGAAACGTA TCACGGAATG ACACGCCCAA GAATCTCACA AAGAACACGG ATGGGACCTA 
     4261 TAATTACACA AGCTTGTTCC TGGTGAACTC ATCTGCTCAT AGAGAGGACG TGGTGTTCAC 
     4321 GTGCCAGGTG AAGCACGACC AACAGCCAGC GATCACCCGA AACCATACCG TGCTGGGATT 
     4381 TGCCCACTCG AGTGATCAAG GGAGCATGCA AACCTTCCCT GATAATAATG CTACCCACAA 
     4441 CTGGAATGTC TTCATCGGTG TGGGCGTGGC GTGTGCTTTG CTCGTAGTCC TGCTGATGGC 
     4501 TGCTCTCTAC CTCCTCCGGA TCAAACAGAA GAAAGCCAAG GGGTCAACAT CTTCCACACG 
     4561 GTTGCACGAG CCCGAGAAGA ACGCCAGGGA AATAACCCAG ATCCAGGACA CAAATGACAT 
     4621 CAACGACATC ACATACGCAG ACCTGAATCT GCCCAAAGAG AAGAAGCCCG CACCCCGGGC 
     4681 CCCTGAGCCT AACAACCACA CAGAATATGC AAGCATTGAG ACAGGCAAAG TGCCTAGGCC 
     4741 AGAGGATACC CTCACCTATG CTGACCTGGA CATGGTCCAC CTCAGCCGGG CACAGCCAGC 
     4801 CCCCAAGCCT GAGCCATCTT TCTCAGAGTA TGCTAGTGTC CAGGTCCAGA GGAAGACGCG 
     4861 TACGCGGCCG CTCGAGATGA GCGGGGGCGA GGAGCTGTTC GCCGGCATCG TGCCCGTGCT 
     4921 GATCGAGCTG GACGGCGACG TGCACGGCCA CAAGTTCAGC GTGCGCGGCG AGGGCGAGGG 
     4981 CGACGCCGAC TACGGCAAGC TGGAGATCAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT 
     5041 GCCCTGGCCC ACCCTGGTGA CCACCCTCTG CTACGGCATC CAGTGCTTCG CCCGCTACCC 
     5101 CGAGCACATG AAGATGAACG ACTTCTTCAA GAGCGCCATG CCCGAGGGCT ACATCCAGGA 
     5161 GCGCACCATC CAGTTCCAGG ACGACGGCAA GTACAAGACC CGCGGCGAGG TGAAGTTCGA 
     5221 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCAAG GACTTCAAGG AGGACGGCAA 
     5281 CATCCTGGGC CACAAGCTGG AGTACAGCTT CAACAGCCAC AACGTGTACA TCCGCCCCGA 
     5341 CAAGGCCAAC AACGGCCTGG AGGCTAACTT CAAGACCCGC CACAACATCG AGGGCGGCGG 
     5401 CGTGCAGCTG GCCGACCACT ACCAGACCAA CGTGCCCCTG GGCGACGGCC CCGTGCTGAT 
     5461 CCCCATCAAC CACTACCTGA GCACTCAGAC CAAGATCAGC AAGGACCGCA ACGAGGCCCG 
     5521 CGACCACATG GTGCTCCTGG AGTCCTTCAG CGCCTGCTGC CACACCCACG GCATGGACGA 
     5581 GCTGTACAGG TCCGGACTCA GAGTTTGGGT AGGAAGCGGA GCTACTAACT TCAGCCTGCT 
     5641 GAAGCAGGCT GGAGACGTGG AGGAGAACCC TGGACCTATG ACCGAGTACA AGCCCACGGT 
     5701 GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC 
     5761 CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGATCGC CACATCGAGC GGGTCACCGA 
     5821 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA 
     5881 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT 
     5941 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA 
     6001 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT 
     6061 CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT 
     6121 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT 
     6181 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC 
     6241 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGATGT ACACCCAAAC GGCCGGCCGC 
     6301 GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG 
     6361 AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG 
     6421 ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 
     6481 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 
     6541 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 
     6601 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 
     6661 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 
     6721 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 
     6781 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC 
     6841 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 
     6901 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 
     6961 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC 
     7021 GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT 
     7081 ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC 
     7141 ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT 
     7201 TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT 
     7261 GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG 
     7321 CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA 
     7381 GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG 
     7441 CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA 
     7501 TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG GTTAGACCAG 
     7561 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC 
     7621 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA 
     7681 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC AAAAGGCCAG 
     7741 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 
     7801 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 
     7861 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 
     7921 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 
     7981 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 
     8041 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 
     8101 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 
     8161 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 
     8221 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 
     8281 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 
     8341 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 
     8401 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC GCCCCGCCCT 
     8461 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC 
     8521 AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT 
     8581 ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA 
     8641 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT 
     8701 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA 
     8761 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT 
     8821 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG 
     8881 CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT 
     8941 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG 
     9001 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC 
     9061 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC TTCCTTTTTC 
     9121 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     9181 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC
//