LOCUS       MR207670                6324 bp    DNA     circular  SYN 
DEFINITION  Neu4 (Myc-DDK-tagged) - Mouse sialidase 4 (Neu4)
ACCESSION   NM_173772
VERSION     NM_173772.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2479..2570 
                     /label="Myc-DDK"  
     misc_feature    2600..3185 
                     /label="PolyA signal"  
     misc_feature    2657..2676 
                     /label="Restriction Enzyme"  
     misc_feature    3461..4133 
                     /label="ColE1"  
     misc_feature    4563..5357 
                     /label="Kan/Neo"  
     misc_feature    5392..5694 
                     /label="SV40 ori"  
     misc_feature    5780..6257 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2454..2461 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2454 
                     /label="Neu4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCCACG CGTGTTCCCA GGAGAACGGT GCTCTTCCAG AGGGAAAGGA 
     1081 CGGGCCTGAC CTACCGTGTG CCTGCGTTAC TCTGTGTGCC TCCCAGGCCT ACTCTGCTGG 
     1141 CCTTCGCGGA ACAGCGACTT AGCCCTGATG ACTCCCATGC CCACCGCCTG GTGCTACGGA 
     1201 GGGGCACGCT GACCAGGGGC TCAGTGCGGT GGGGCACTCT GAGTGTACTG GAGACTGCAG 
     1261 TACTGGAGGA GCACAGGTCT ATGAACCCTT GCCCGGTGCT GGATGAGCAC TCTGGTACCA 
     1321 TCTTCCTCTT CTTCATTGCC GTGCTGGGCC ACACACCGGA GGCCGTGCAA ATCGCCACTG 
     1381 GCAAGAACGC TGCTCGCCTC TGCTGTGTGA CCAGCTGTGA CGCTGGCCTC ACCTGGGGCA 
     1441 GTGTTCGAGA TCTCACTGAG GAAGCCATTG GTGCTGCATT GCAGGACTGG GCCACCTTTG 
     1501 CTGTGGGTCC GGGCCATGGA GTTCAGCTGC GCTCGGGTCG CCTGCTTGTT CCTGCTTACA 
     1561 CCTATCATGT GGACCGACGG GAATGTTTTG GCAAGATCTG CTGGACCAGT CCCCACTCCT 
     1621 TGGCATTCTA CAGTGATGAT CATGGGATCT CCTGGCATTG TGGAGGCCTT GTGCCCAACC 
     1681 TACGCTCTGG AGAGTGCCAA CTGGCTGCGG TAGATGGAGA CTTTCTCTAC TGTAATGCTC 
     1741 GAAGCCCTCT GGGTAACCGT GTGCAGGCAC TGAGTGCTGA TGAAGGCACG TCCTTCCTAC 
     1801 CAGGGGAGCT GGTGCCTACA TTGGCAGAGA CGGCTCGTGG TTGCCAGGGT AGCATTGTGG 
     1861 GCTTCCTAGC TCCACCCTCA ATCGAGCCTC AGGATGACCG GTGGACAGGG AGTCCTAGGA 
     1921 ACACCCCACA TTCCCCATGC TTCAATCTCA GAGTACAGGA GTCTTCGGGG GAAGGTGCCA 
     1981 GAGGTCTTCT TGAACGTTGG ATGCCCAGGT TGCCTCTCTG CTACCCACAG TCCCGGAGCC 
     2041 CAGAGAATCA TGGCCTAGAG CCTGGGTCAG ATGGAGATAA GACATCCTGG ACTCCGGAAT 
     2101 GTCCTATGTC CTCTGATTCC ATGCTTCAGA GCCCCACATG GCTACTATAT TCCCACCCAG 
     2161 CAGGGCGTAG AGCTCGGCTC CACATGGGAA TCTACCTGAG CCGATCCCCC TTGGATCCCC 
     2221 ACAGCTGGAC AGAGCCCTGG GTGATCTATG AGGGCCCCAG TGGCTACTCT GACCTTGCCT 
     2281 TTCTTGGGCC TATGCCTGGG GCATCCCTGG TTTTTGCCTG TCTGTTTGAG AGCGGGACCA 
     2341 GGACTTCCTA TGAAGACATT TCTTTTTGCT TGTTCTCACT GGCGGATGTC CTGGAGAATG 
     2401 TGCCCACTGG CTTAGAGATG CTAAGTCTCA GGGATAAGGC TCAGGGGCAT TGCTGGCCCT 
     2461 CTAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     2521 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     2581 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     2641 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     2701 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     2761 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     2821 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     2881 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     2941 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3001 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3061 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3121 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3181 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3241 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3301 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3361 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     3421 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     3481 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     3541 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     3601 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     3661 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     3721 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     3781 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     3841 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     3901 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     3961 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4021 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4081 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4141 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4201 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4261 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4321 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4381 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     4441 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     4501 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     4561 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     4621 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     4681 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     4741 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     4801 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     4861 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     4921 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     4981 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5041 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5101 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5161 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5221 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5281 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5341 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     5401 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     5461 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     5521 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     5581 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     5641 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     5701 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     5761 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     5821 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     5881 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     5941 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6001 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6061 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6121 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6181 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6241 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6301 TAACAAAAAT TTAACGCGAA TTTT
//