LOCUS       MR206604L4                8963 bp    DNA     circular  SYN 
DEFINITION  Pgk2 (Myc-DDK-tagged) - Mouse phosphoglycerate kinase 2 (Pgk2)
ACCESSION   NM_031190
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7254..7434 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8162..8808 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6129..6149 
                     /label="LR50"  
     misc_feature    4592..5320 
                     /label="mGFP"  
     misc_feature    5327..5392 
                     /label="P2A"  
     misc_feature    7482..8101 
                     /label="pBR322 Ori"  
     misc_feature    5393..5992 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4571..4577 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4571 
                     /label="Pgk2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCTCTTTCT GCTAAGTTGA CTCTGGACAA 
     3361 AGTGGATCTT AAGGGAAAAA GAGTAATCAT GAGAGTAGAC TTCAACGTTC CCATGAAGAA 
     3421 TAACCAAATT ACAAACAACC AGAGAATCAA GGCTGCCATC CCAAGTATCA AGCACTGTCT 
     3481 GGACAATGGA GCCAAGTCCG TAGTTCTCAT GAGTCACCTC GGTCGGCCTG ATGGTATCCC 
     3541 TATGCCAGAC AAGTATTCAT TAGAGCCTGT TGCTGATGAG CTCAAGTCCC TGCTGAACAA 
     3601 GGACGTCATA TTCTTGAAGG ACTGTGTGGG CCCTGAAGTA GAGCAAGCCT GTGCCAACCC 
     3661 AGATAATGGG TCTATCATCC TGCTGGAGAA CCTGCGCTTC CATGTGGAGG AAGAAGGTAA 
     3721 GGGTAAAGAT TCTTCTGGAA AAAAGATTAG TGCTGACCCT GCTAAAGTAG AAGCCTTCCA 
     3781 AGCATCACTG TCTAAACTTG GCGATGTCTA TGTCAACGAT GCATTTGGCA CTGCACATCG 
     3841 GGCTCACAGT TCTACGGTCG GAGTAAATTT GCCCCAGAAG GCATCTGGTT TCCTTATGAA 
     3901 GAAGGAACTG GATTATTTTT CCAAGGCTTT AGAAAAGCCA GAGAGGCCCT TCCTGGCTAT 
     3961 CCTTGGTGGA GCCAAAGTGA AAGACAAGAT CCAACTCATT AAAAATATGT TAGACAAAGT 
     4021 CAATTTCATG ATTATTGGTG GTGGAATGGC TTACACCTTC CTGAAAGAAC TCAAGAACAT 
     4081 GCAGATTGGT GCTTCCTTGT TTGATGAAGA GGGAGCCACG ATTGTTAAAG AGATCATGGA 
     4141 AAAAGCAGAA AAGAATGGTG TAAAGATAGT TTTTCCTGTT GACTTTGTTA CTGGTGACAA 
     4201 GTTTGATGAG AATGCTAAAG TTGGACAAGC CACTATAGAA TCTGGTATAC CATCTGGTTG 
     4261 GATGGGCTTG GACTGTGGCC CTGAGAGCAT TAAAATCAAT GCTCAAATTG TGGCCCAAGC 
     4321 AAAGCTGATA GTTTGGAATG GACCTATTGG GGTATTTGAA TGGGATGCCT TTGCTAAAGG 
     4381 AACCAAAGCT CTCATGGATG AAGTTGTAAA GGCCACCTCC AATGGCTGTG TCACCATTAT 
     4441 AGGAGGCGGA GATACTGCTA CTTGCTGCGC CAAATGGGGC ACTGAAGACA AGGTCAGCCA 
     4501 TGTGAGCACA GGAGGTGGGG CAAGTCTTGA GCTTCTGGAA GGTAAAATCC TTCCAGGGGT 
     4561 AGAGGCCCTC AGCAACATGA CGCGTACGCG GCCGCTCGAG ATGAGCGGGG GCGAGGAGCT 
     4621 GTTCGCCGGC ATCGTGCCCG TGCTGATCGA GCTGGACGGC GACGTGCACG GCCACAAGTT 
     4681 CAGCGTGCGC GGCGAGGGCG AGGGCGACGC CGACTACGGC AAGCTGGAGA TCAAGTTCAT 
     4741 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG GTGACCACCC TCTGCTACGG 
     4801 CATCCAGTGC TTCGCCCGCT ACCCCGAGCA CATGAAGATG AACGACTTCT TCAAGAGCGC 
     4861 CATGCCCGAG GGCTACATCC AGGAGCGCAC CATCCAGTTC CAGGACGACG GCAAGTACAA 
     4921 GACCCGCGGC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 
     4981 CAAGGACTTC AAGGAGGACG GCAACATCCT GGGCCACAAG CTGGAGTACA GCTTCAACAG 
     5041 CCACAACGTG TACATCCGCC CCGACAAGGC CAACAACGGC CTGGAGGCTA ACTTCAAGAC 
     5101 CCGCCACAAC ATCGAGGGCG GCGGCGTGCA GCTGGCCGAC CACTACCAGA CCAACGTGCC 
     5161 CCTGGGCGAC GGCCCCGTGC TGATCCCCAT CAACCACTAC CTGAGCACTC AGACCAAGAT 
     5221 CAGCAAGGAC CGCAACGAGG CCCGCGACCA CATGGTGCTC CTGGAGTCCT TCAGCGCCTG 
     5281 CTGCCACACC CACGGCATGG ACGAGCTGTA CAGGTCCGGA CTCAGAGTTT GGGTAGGAAG 
     5341 CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA ACCCTGGACC 
     5401 TATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT 
     5461 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA 
     5521 TCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA 
     5581 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA 
     5641 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG 
     5701 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA 
     5761 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG 
     5821 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT 
     5881 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC 
     5941 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG 
     6001 ATGTACACCC AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA 
     6061 ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG 
     6121 TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 
     6181 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 
     6241 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 
     6301 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 
     6361 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 
     6421 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 
     6481 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 
     6541 AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 
     6601 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 
     6661 CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT 
     6721 GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA 
     6781 ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG 
     6841 GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG 
     6901 GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC 
     6961 TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT 
     7021 GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC 
     7081 TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC 
     7141 CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG 
     7201 TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT 
     7261 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC 
     7321 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG 
     7381 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT 
     7441 AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 
     7501 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 
     7561 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 
     7621 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 
     7681 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 
     7741 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 
     7801 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 
     7861 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 
     7921 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 
     7981 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 
     8041 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 
     8101 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 
     8161 TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA 
     8221 TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA 
     8281 GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT 
     8341 CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA 
     8401 AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA 
     8461 CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG 
     8521 ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA 
     8581 TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG 
     8641 CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA 
     8701 AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG 
     8761 CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT 
     8821 TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8881 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8941 TTCCCCGAAA AGTGCCACCT GAC
//