LOCUS       MR205137                5908 bp    DNA     circular  SYN 
DEFINITION  Klc4 (Myc-DDK-tagged) - Mouse kinesin light chain 4 (cDNA clone MGC:11935 IMAGE:3599898)
ACCESSION   BC005746
VERSION     BC005746
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2065..2156 
                     /label="Myc-DDK"  
     misc_feature    2186..2771 
                     /label="PolyA signal"  
     misc_feature    2243..2262 
                     /label="Restriction Enzyme"  
     misc_feature    3047..3719 
                     /label="ColE1"  
     misc_feature    4149..4943 
                     /label="Kan/Neo"  
     misc_feature    4978..5280 
                     /label="SV40 ori"  
     misc_feature    5366..5843 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2047..2053 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2047 
                     /label="Klc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGGCTTG GTGTTGGGGC AGCGGGATGA GCCTGCAGGG CACCGGCTCA 
     1081 GTCAAGAGGA GATCTTGGGC AGCACCAAGG TGGTGAGCCA AGGGCTGGAG GCCCTACACA 
     1141 GTGAACATCA GGCAGTGCTG CAGAGCCTGT CCCACACCAT TGAGTGTCTG CAACAGGGAG 
     1201 GCCATGAGGA AGGACTGGTC CATGAGAAGG CCCGGCAGCT GCGGCGGTCT ATGGAGAACA 
     1261 TCGAGCTGGG GCTGAGCGAG GCCCAGGTGA TGCTGGCGCT GGCTAGCCAC CTGAGCACAG 
     1321 TGGAGTCTGA GAAGCAGAAG CTGCGAGCCC AAGTGCGGCG GCTATGCCAG GAGAATCAGT 
     1381 GGCTCCGGGA TGAGCTGGCG GGCACTCAGC AGCGCCTGCA GCGCAGCGAG CAGGCTGTGG 
     1441 CTCAGCTAGA GGAAGAGAAG AAGCACCTGG AGTTCCTGCG GCAGCTGCGG CAGTATGATG 
     1501 AGGACGGCCA CGGCATGGAG GAGAAGGAGG GAGAGGCCAC CAAAGATTCT CTGGATGACC 
     1561 TGTTCCCCAA TGAGGAGGAG GAGGACTCCG GCAACGACTT GTCCCGTGGT CAGGGTGCTG 
     1621 CAGCGGCCCA GCAGGGTGGA TATGAGATCC CAGCAAGGTT GCGGACCCTG CACAACCTGG 
     1681 TCATCCAGTA TGCAGCTCAG GGTCGCTATG AGGTGGCGGT GCCACTCTGC AAGCAAGCGC 
     1741 TAGAGGACCT GGAGCGCACA TCTGGCCGAG GTCACCCTGA TGTGGCTACC ATGCTCAACA 
     1801 TCCTGGCTCT GGTGTACCGG GACCAGAATA AGTACAAGGA GGCTGCTCAT CTGCTGAACG 
     1861 ATGCTCTCAG CATCCGGGAG AGCACCCTGG GCCGGGACCA CCCAGCCGTG GCTGCCACAC 
     1921 TCAACAATCT GGCTCTTCTG TCAGTTTGGG GCTGGCCAAC TGGGCTGCCT TGCCCTGGTC 
     1981 TCTTTTACCC TCTGCTGCCT CACTACAGGT CCACATATTT CACTGTTTCT TTAATAAATG 
     2041 AACCAGGTAA CATTTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT 
     2101 CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC 
     2161 CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT 
     2221 GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT 
     2281 AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT 
     2341 GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA 
     2401 ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA 
     2461 AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT 
     2521 CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC 
     2581 CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC 
     2641 GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA 
     2701 CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT 
     2761 GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC 
     2821 GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     2881 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     2941 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     3001 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     3061 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     3121 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     3181 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     3241 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     3301 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     3361 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     3421 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     3481 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     3541 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     3601 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     3661 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG 
     3721 CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT 
     3781 GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT 
     3841 CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT 
     3901 GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT 
     3961 TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC 
     4021 GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA 
     4081 AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT 
     4141 CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG 
     4201 CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG 
     4261 CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG 
     4321 CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA 
     4381 GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC 
     4441 GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG 
     4501 ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG 
     4561 GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT 
     4621 CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG 
     4681 CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT 
     4741 GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC 
     4801 GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA 
     4861 GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG 
     4921 AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG 
     4981 ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT 
     5041 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG 
     5101 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA 
     5161 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG 
     5221 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG 
     5281 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC 
     5341 CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 
     5401 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 
     5461 CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT 
     5521 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT 
     5581 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC 
     5641 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA 
     5701 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC 
     5761 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT 
     5821 CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT 
     5881 GATTTAACAA AAATTTAACG CGAATTTT
//