LOCUS       MG227175                9425 bp    DNA     circular  SYN 
DEFINITION  Cyld (tGFP-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld) transcript variant 3, (10ug)
ACCESSION   NM_001128170
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3906..4610 
                     /label="GFP"  
     misc_feature    4657..5242 
                     /label="PolyA signal"  
     misc_feature    7334..7979 
                     /label="ColE1"  
     misc_feature    5696..6490 
                     /label="Neo"  
     misc_feature    5327..5629 
                     /label="SV40 ori"  
     misc_feature    7994..8854 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3885..3891 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3885 
                     /label="Cyld"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCAGGC CTGTGGAGCC AAGAGAAAGT TACTTCACCC TACTGGGAAG 
     1081 AACGGATTTT TTATCTGCTT CTTCAAGAAT GCAGTGTAAC AGACAAACAA ACTCAGAAGC 
     1141 TGCTGAAAGT ACCCAAAGGG AGTATAGGAC AGTACATCCA AGACCGTTCT GTGGGGCATT 
     1201 CAAGAGTTCC TTCCACAAAA GGCAAGAAAA ATCAGATTGG ATTAAAAATC TTGGAGCAAC 
     1261 CGCATGCAGT TCTGTTTGTT GATGAAAAGG ATGTTGTAGA AATAAATGAA AAATTCACAG 
     1321 AGTTACTGTT GGCAATTACC AACTGTGAGG AGAGGCTCAG CCTATTTAGA AACAGACTCC 
     1381 GACTAAGTAA AGGCCTCCAG GTAGACGTGG GCAGTCCTGT GAAAGTACAG CTGCGATCTG 
     1441 GGGAAGAGAA ATTTCCAGGA GTTGTACGCT TCAGAGGACC TTTATTAGCG GAGAGGACGG 
     1501 TGTCGGGGAT TTTCTTTGGA GTAGAATTAT TGGAAGAAGG TCGTGGTCAA GGTTTCACGG 
     1561 ATGGGGTATA TCAAGGGAAG CAGCTTTTCC AGTGTGATGA GGACTGTGGC GTGTTTGTTG 
     1621 CATTGGACAA ACTAGAACTT ATAGAAGATG ATGACAATGG ATTGGAAAGT GATTTTGCAG 
     1681 GCCCAGGAGA TACAATGCAG GTTGAACCTC CCCCTTTGGA AATAAACTCC AGAGTTTCTT 
     1741 TGAAGGTTGG AGAAAGTACA GAATCTGGAA CAGTAATATT CTGTGATGTT TTACCAGGAA 
     1801 AAGAGAGTCT AGGATATTTT GTTGGTGTGG ACATGGATAA CCCTATTGGC AACTGGGATG 
     1861 GAAGGTTTGA TGGAGTACAG CTCTGTAGTT TTGCAAGTGT TGAAAGTACA ATTCTCCTGC 
     1921 ACATCAATGA CATCATCCCA GCTTTATCAG ATAGCGTGAC ACAGGAAAGG AGGCCTCCCA 
     1981 AACTTGCCTT TATGTCAAGA GGTGTAGGTG ACAAAGGTTC ATCTAGTCAT AATAAACCAA 
     2041 AGGTTACAGG ATCTACCTCA GACCCTGGAA GTAGAAACAG ATCTGAATTA TTTTATACCT 
     2101 TAAATGGGTC ATCTGTTGAC TCACAACAAT CCAAGTCCAA AAATCCATGG TACATTGATG 
     2161 AAGTTGCAGA AGACCCTGCA AAGTCACTTA CAGAGATGTC TTCGGACTTC GGACATTCAT 
     2221 CTCCTCCACC GCAGCCTCCT TCCATGAACT CCTTGTCTAG CGAGAACAGA TTCCACTCCT 
     2281 TACCCTTCAG CCTGACAAAG ATGCCCAATA CTAATGGCAG CATGGCTCAT AGTCCACTCT 
     2341 CTCTGTCAGT GCAGTCTGTG ATGGGGGAGC TGAACAGCAC ACCTGTCCAG GAGAGTCCAC 
     2401 CCTTGCCCAT CTCTTCTGGG AATGCACACG GGCTAGAGGT GGGCTCACTG GCTGAAGTAA 
     2461 AAGAGAACCC CCCGTTCTAT GGGGTTATCC GTTGGATTGG CCAGCCACCA GGGCTCAGTG 
     2521 ACGTGCTAGC TGGACTGGAA CTGGAAGATG AATGCGCAGG CTGTACAGAT GGAACTTTCA 
     2581 GGGGCACGCG GTATTTCACG TGTGCCCTGA AGAAGGCACT GTTTGTGAAA CTGAAGAGCT 
     2641 GCAGACCGGA CTCTAGGTTT GCATCCTTGC AGCCTGTTTC CAATCAAATT GAAAGGTGTA 
     2701 ACTCTTTAGC ATTTGGGGGC TATTTAAGTG AAGTAGTAGA AGAAAATACT CCACCTAAAA 
     2761 TGGAAAAGGA AGGTTTAGAG ATAATGATTG GAAAGAAGAA AGGCATCCAG GGCCATTACA 
     2821 ATTCTTGTTA CTTAGACTCA ACTTTATTCT GCTTATTTGC TTTTAGTTCT GCCCTGGACA 
     2881 CTGTGTTACT TAGACCCAAA GAGAAGAATG ATATAGAGTA TTACAGTGAG ACTCAGGAGC 
     2941 TACTGAGGAC AGAGATAGTC AATCCTCTGA GAATATATGG ATATGTGTGT GCCACAAAGA 
     3001 TTATGAAACT GAGGAAAATA CTTGAAAAAG TTGAGGCTGC ATCAGGTTTT ACCTCTGAAG 
     3061 AAAAAGATCC TGAAGAATTC TTAAATATCC TGTTTCATGA TATTTTAAGG GTTGAACCAT 
     3121 TGTTAAAAAT AAGATCAGCA GGTCAAAAAG TTCAAGACTG TAACTTCTAT CAAATTTTTA 
     3181 TGGAAAAAAA TGAGAAAGTT GGAGTACCCA CAATTCAGCA GTTATTAGAA TGGTCTTTTA 
     3241 TCAACAGCAA CCTGAAATTT GCAGAGGCAC CATCATGCTT GATTATCCAG ATGCCTCGGT 
     3301 TTGGAAAAGA CTTTAAACTA TTTAAAAAAA TTTTTCCTTC CCTGGAATTA AATATAACAG 
     3361 ATTTACTTGA AGACACTCCC AGGCAGTGCC GCATCTGTGG AGGACTTGCG ATGTACGAGT 
     3421 GCAGGGAGTG CTATGACGAT CCGGACATCT CAGCTGGGAA GATCAAGCAG TTCTGTAAGA 
     3481 CCTGCAGCAC TCAGGTTCAC CTTCATCCCA GGAGGTTGAA TCATTCTTAT CATCCAGTAT 
     3541 CACTTCCCAA AGACTTGCCT GACTGGGACT GGAGACACGG CTGCATCCCC TGTCAGAAGA 
     3601 TGGAGTTATT TGCTGTTCTC TGCATAGAAA CGAGCCACTA TGTTGCTTTT GTGAAGTATG 
     3661 GGAAGGATGA CTCTGCCTGG CTTTTCTTTG ACAGCATGGC GGATCGAGAT GGTGGTCAGA 
     3721 ATGGCTTCAA CATTCCACAA GTGACGCCCT GCCCAGAAGT GGGAGAGTAC TTGAAGATGT 
     3781 CTCTGGAGGA CCTGCACTCT TTGGATTCCA GAAGGATTCA AGGCTGTGCG CGCAGACTTC 
     3841 TTTGCGATGC ATACATGTGC ATGTACCAGA GTCCAACCAT GAGCCTGTAC AAAACGCGTA 
     3901 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3961 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     4021 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     4081 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     4141 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4201 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4261 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4321 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4381 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4441 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4501 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4561 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5341 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5401 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5461 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5521 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5581 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5641 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5701 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5761 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5821 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5881 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5941 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     6001 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     6061 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     6121 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6181 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6241 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6301 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6361 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6421 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6481 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6541 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6601 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6661 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6721 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6781 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6961 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     7021 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     7081 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7141 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7201 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7261 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7321 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7381 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7441 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7501 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7561 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7621 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7681 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7741 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7801 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7861 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7921 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7981 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     8041 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     8101 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     8161 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8221 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8281 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8341 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8401 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8461 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8521 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8581 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8641 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8701 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8761 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8821 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8881 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8941 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     9001 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     9061 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     9121 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9181 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9241 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9301 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9361 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9421 ATTTT
//