LOCUS       MG226912                9491 bp    DNA     circular  SYN 
DEFINITION  Trpc4 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily C member 4 (Trpc4), (10ug)
ACCESSION   NM_016984
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3970..4674 
                     /label="GFP"  
     misc_feature    4721..5306 
                     /label="PolyA signal"  
     misc_feature    7398..8043 
                     /label="ColE1"  
     misc_feature    5760..6554 
                     /label="Neo"  
     misc_feature    5391..5693 
                     /label="SV40 ori"  
     misc_feature    8058..8918 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3942..3949 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3942 
                     /label="Trpc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCAGTTC TATTACAAAA GAAATGTCAA CGCCCCCTAC AGAGACCGCA 
     1081 TCCCACTGAG GATTGTCAGA GCAGAATCTG AGCTCTCACC ATCAGAGAAA GCCTACTTGA 
     1141 ATGCTGTGGA GAAGGGGGAC TATGCAAGCG TCAAGAAGTC TCTGGAGGAA GCTGAGATTT 
     1201 ATTTTAAAAT CAACATTAAC TGCATCGACC CCCTGGGAAG GACCGCCCTC CTCATTGCCA 
     1261 TTGAAAATGA GAATCTGGAG CTTATTGAAC TATTGTTGAG TTTCAATGTC TATGTAGGCG 
     1321 ATGCGCTGCT TCACGCCATC AGAAAAGAGG TGGTTGGAGC CGTGGAGCTA CTGCTGAACC 
     1381 ACAAAAAGCC AAGTGGAGAG AAGCAGGTGC CTCCCATTCT CCTTGATAAA CAGTTCTCTG 
     1441 AATTCACTCC GGACATCACA CCCATCATCT TGGCTGCACA TACAAATAAT TACGAGATAA 
     1501 TCAAACTTTT GGTTCAGAAA GGTGTCTCAG TGCCCAGACC CCACGAGGTC CGCTGTAACT 
     1561 GTGTTGAGTG TGTCTCCAGC TCGGATGTGG ACAGCCTCAG GCATTCACGG TCCAGGCTCA 
     1621 ACATCTACAA GGCCTTGGCC AGCCCCTCGC TCATTGCCCT GTCAAGCGAA GACCCTTTCC 
     1681 TTACTGCCTT TCAGTTAAGT TGGGAGCTGC AAGAACTCAG CAAGGTGGAG AACGAATTCA 
     1741 AGTCGGAGTA TGAGGAGCTG TCTAGACAGT GCAAACAATT TGCCAAGGAC CTCCTAGATC 
     1801 AGACACGGAG TTCCAGAGAG CTGGAAATCA TTCTTAATTA CCGTGATGAC AATAGTCTGA 
     1861 TCGAAGAACA GAGTGGAAAT GATCTTGCAA GGCTAAAATT AGCCATTAAG TACCGTCAAA 
     1921 AAGAGTTTGT TGCTCAGCCC AACTGCCAGC AGCTGCTCGC TTCCCGCTGG TACGATGAGT 
     1981 TCCCAGGCTG GAGGAGAAGA CACTGGGCGG TGAAGATGGT GACATGTTTC ATAATAGGAC 
     2041 TACTCTTCCC CGTCTTCTCC GTGTGCTACC TGATAGCTCC CAAAAGCCCA CTTGGACTGT 
     2101 TCATCAGAAA GCCATTTATC AAGTTTATCT GCCACACAGC CTCCTATCTG ACCTTTTTGT 
     2161 TTCTGCTGCT GCTAGCCTCT CAGCACATCG ACAGGTCAGA CTTGAACAGG CAAGGTCCAC 
     2221 CACCAACCAT CGTGGAGTGG ATGATATTAC CGTGGGTCCT GGGTTTTATA TGGGGAGAGA 
     2281 TTAAACAGAT GTGGGATGGC GGACTCCAGG ATTACATCCA TGACTGGTGG AATCTAATGG 
     2341 ACTTTGTGAT GAACTCCTTG TATCTGGCAA CAATCTCCTT GAAGATTGTC GCGTTTGTAA 
     2401 AGTACAGTGC TCTGAACCCA CGGGAATCAT GGGACATGTG GCACCCCACC CTGGTGGCAG 
     2461 AGGCATTATT TGCTATTGCA AACATCTTCA GTTCCCTCCG CCTGATCTCT CTGTTCACTG 
     2521 CCAATTCTCA CCTGGGGCCT CTGCAGATAT CTCTGGGAAG GATGCTTCTG GACATCCTGA 
     2581 AGTTCTTGTT CATCTACTGC CTCGTGCTGC TAGCTTTTGC AAATGGCCTA AATCAGCTGT 
     2641 ACTTTTACTA TGAAGAAACA AAGGGGCTAA GCTGCAAAGG CATCCGGTGC GAGAAACAGA 
     2701 ACAACGCGTT TTCCACGTTA TTCGAGACAC TACAGTCCCT GTTTTGGTCA ATATTTGGAC 
     2761 TCATCAATCT CTATGTTACC AATGTCAAAG CCCAGCACGA GTTCACTGAG TTTGTTGGGG 
     2821 CCACCATGTT TGGCACATAT AATGTCATCT CTCTGGTTGT CCTGCTGAAC ATGTTAATTG 
     2881 CTATGATGAA TAATTCTTAC CAACTAATTG CCGACCATGC AGATATAGAA TGGAAATTTG 
     2941 CTCGAACAAA GCTTTGGATG AGCTACTTTG AAGAAGGAGG TACCCTGCCT ACACCTTTCA 
     3001 ATGTCATCCC AAGCCCCAAG TCCCTGTGGT ACCTGGTCAA GTGGATATGG ACACACTTAT 
     3061 GTAAGAAAAA AATGAGAAGG AAGCCAGAAA GCTTCGGGAC AATTGGGCGG CGTGCTGCTG 
     3121 ATAACTTGAG AAGACATCAC CAATACCAAG AGGTGATGAG GAACCTGGTG AAGCGGTACG 
     3181 TGGCAGCCAT GATCAGAGAG GCAAAAACCG AAGAAGGCTT GACGGAGGAG AATGTTAAGG 
     3241 AACTAAAGCA AGACATTTCT AGCTTCCGCT TCGAAGTTCT GGGATTGCTC AGAGGAAGCA 
     3301 AGCTCTCTAC AATACAGTCA GCCAACGCGG CGAGTTCAGC GGACTCCGAC GAGAAGAGCC 
     3361 AGAGCGAAGG TAATGGCAAG GACAAGAGAA AGAATCTCAG CCTCTTTGAT TTAACCACTC 
     3421 TGATCCACCC GCGGTCGGCA GCCATTGCCT CCGAGAGACA TAACCTAAGC AATGGTTCCG 
     3481 CCCTGGTGGT GCAGGAGCCG CCCAGGGAGA AGCAGAGGAA AGTGAATTTT GTGGCTGATA 
     3541 TCAAAAACTT CGGGTTATTT CATAGACGGT CAAAACAAAA TGCTGCTGAG CAAAACGCAA 
     3601 ACCAAATCTT CTCTGTTTCA GAAGAAATTA CTCGTCAACA GGCGGCAGGA GCACTTGAGC 
     3661 GAAATATCGA ACTGGAATCC AAAGGATTAG CTTCACGGGG TGACCGCAGC ATTCCTGGTC 
     3721 TCAATGAACA GTGTGTGCTA GTAGACCATA GAGAAAGGAA TACGGACACT TTGGGTTTAC 
     3781 AGGTAGGCAA GAGAGTGTGC TCCACCTTCA AGTCGGAGAA GGTGGTGGTG GAAGACACCG 
     3841 TCCCTATTAT ACCAAAGGAG AAACACGCCC ACGAGGAGGA CTCGAGCATA GACTATGACT 
     3901 TAAGCCCCAC GGACACAGCT GCCCACGAAG ATTATGTGAC CACAAGATTG AGCGGACCGA 
     3961 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     4021 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     4081 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     4141 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     4201 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     4261 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     4321 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     4381 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     4441 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     4501 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     4561 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4621 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4681 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4741 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4801 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4861 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4921 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4981 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5041 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5101 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5161 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5221 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5281 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5341 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     5401 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5461 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5521 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     5581 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5641 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5701 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5761 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5821 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5881 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5941 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     6001 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     6061 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     6121 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     6181 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     6241 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     6301 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     6361 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     6421 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     6481 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     6541 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6601 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6661 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6721 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6781 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6841 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6901 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6961 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     7021 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     7081 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     7141 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     7201 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     7261 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     7321 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     7381 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     7441 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     7501 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     7561 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7621 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7681 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7741 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7801 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7861 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7921 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7981 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     8041 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     8101 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     8161 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     8221 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     8281 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     8341 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     8401 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     8461 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     8521 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     8581 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8641 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8701 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8761 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8821 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8881 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8941 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     9001 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     9061 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     9121 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     9181 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     9241 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     9301 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     9361 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     9421 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     9481 ACGCGAATTT T
//