LOCUS       MG226846                9095 bp    DNA     circular  SYN 
DEFINITION  Vav1 (tGFP-tagged) - Mouse vav 1 oncogene (Vav1) transcript variant 1, (10ug)
ACCESSION   NM_011691
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3576..4280 
                     /label="GFP"  
     misc_feature    4327..4912 
                     /label="PolyA signal"  
     misc_feature    7004..7649 
                     /label="ColE1"  
     misc_feature    5366..6160 
                     /label="Neo"  
     misc_feature    4997..5299 
                     /label="SV40 ori"  
     misc_feature    7664..8524 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3555..3561 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3555 
                     /label="Vav1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCTGG CGACAGTGCA CCCACTGGCT GATCCAGTGT CGGGTGCTGC 
     1081 CTCCCAGCCA CCGTGTGACC TGGGAGGGGG CCCAGGTGTG TGAGCTGGCA CAGGCACTGC 
     1141 GGGACGGTGT CCTCTTGTGC CAATTGCTTA ACAACCTGCT TCCCCAGGCC ATTAATCTTC 
     1201 GCGAGGTTAA CTTGCGGCCC CAGATGTCCC AGTTCCTTTG TCTTAAGAAC ATTCGAACCT 
     1261 TCCTGTCTAC TTGCTGTGAG AAGTTCGGCC TCAAGCGCAG TGAACTCTTT GAGGCTTTTG 
     1321 ACCTCTTCGA TGTGCAGGAC TTTGGAAAGG TCATCTACAC CCTGTCTGCT CTGTCATGGA 
     1381 CACCCATTGC CCAGAACAAA GGAATCATGC CCTTCCCAAC AGAGGACAGC GCTCTGAACG 
     1441 ACGAAGATAT TTACAGTGGC CTTTCAGACC AGATTGATGA CACCGCAGAG GAAGACGAGG 
     1501 ACCTTTATGA CTGCGTGGAA AATGAGGAGG CAGAGGGGGA CGAGATCTAC GAGGACCTAA 
     1561 TGCGCTTGGA GTCGGTGCCT ACGCCACCCA AGATGACAGA GTATGATAAG CGCTGCTGCT 
     1621 GCCTGCGGGA GATCCAGCAG ACGGAGGAGA AGTATACAGA CACACTGGGC TCCATCCAGC 
     1681 AGCACTTCAT GAAGCCTCTG CAGCGATTCC TTAAGCCTCA AGACATGGAG ACCATCTTTG 
     1741 TCAACATTGA GGAGCTGTTC TCTGTGCATA CCCACTTCTT AAAGGAACTG AAGGATGCCC 
     1801 TGGCTGGCCC GGGAGCAACA ACACTGTATC AGGTCTTCAT CAAGTACAAG GAGAGGTTCC 
     1861 TGGTTTATGG CCGTTATTGC AGTCAGGTGG AGTCAGCCAG CAAGCACTTG GATCAAGTGG 
     1921 CCACAGCACG GGAGGATGTG CAGATGAAGC TGGAGGAATG TTCTCAAAGA GCTAACAATG 
     1981 GCCGATTCAC CCTACGGGAT CTGCTGATGG TACCTATGCA GCGGGTGCTG AAGTACCACC 
     2041 TCCTTCTCCA GGAGCTAGTG AAACACACAC AGGATGCTAC AGAGAAGGAG AACCTGCGGT 
     2101 TGGCCCTGGA CGCCATGAGG GACCTGGCAC AGTGCGTGAA CGAGGTCAAG AGGGACAATG 
     2161 AAACCCTACG GCAGATCACA AACTTTCAGC TGTCCATTGA GAACCTGGAC CAGTCTCTGG 
     2221 CTAACTATGG CCGGCCCAAG ATTGACGGTG AGCTCAAGAT TACCTCAGTG GAGCGTCGCT 
     2281 CAAAGACAGA CAGGTATGCC TTCCTGCTGG ACAAAGCACT GCTCATCTGT AAACGCCGCG 
     2341 GGGACTCTTA CGACCTCAAA GCCTCGGTGA ACTTGCACAG CTTCCAAGTT CGAGATGACT 
     2401 CCTCCGGGGA GCGAGACAAC AAGAAGTGGA GCCATATGTT CCTTCTGATT GAGGATCAAG 
     2461 GCGCCCAGGG CTATGAGCTG TTCTTCAAGA CTCGGGAGCT GAAGAAGAAG TGGATGGAAC 
     2521 AGTTCGAAAT GGCCATCTCC AACATTTACC CAGAGAATGC TACAGCCAAT GGGCATGATT 
     2581 TTCAGATGTT CTCCTTTGAG GAGACCACTT CCTGCAAGGC CTGCCAGATG TTACTCAGAG 
     2641 GCACATTCTA CCAGGGATAT CGCTGTTACA GGTGCCGGGC ACCTGCACAC AAGGAGTGTC 
     2701 TGGGGAGAGT GCCTCCCTGT GGTCGCCATG GGCAAGATTT CGCAGGAACC ATGAAGAAGG 
     2761 ACAAGCTCCA TCGAAGGGCC CAGGACAAGA AAAGGAATGA ATTGGGTCTG CCTAAGATGG 
     2821 AAGTGTTTCA GGAATACTAT GGGATCCCTC CTCCACCTGG AGCCTTTGGG CCATTTTTAC 
     2881 GGCTCAACCC TGGGGACATT GTGGAGCTCA CTAAGGCAGA GGCTGAGCAC AACTGGTGGG 
     2941 AGGGAAGGAA TACTGCTACA AATGAAGTCG GCTGGTTTCC CTGTAACAGA GTGCATCCCT 
     3001 ATGTCCACGG CCCTCCTCAG GACCTGTCTG TGCATCTCTG GTATGCGGGC CCTATGGAAC 
     3061 GAGCAGGCGC TGAGGGCATC CTCACCAACC GTTCTGATGG GACCTATCTG GTGCGGCAGA 
     3121 GGGTGAAAGA TACAGCGGAG TTCGCCATCA GCATTAAGTA TAACGTGGAG GTCAAGCATA 
     3181 TTAAAATCAT GACGTCAGAG GGGTTGTACC GGATCACAGA GAAGAAGGCT TTCCGGGGCC 
     3241 TTCTGGAACT GGTAGAGTTT TATCAGCAGA ATTCCCTCAA AGATTGCTTC AAGTCGTTGG 
     3301 ACACCACCTT GCAGTTTCCT TATAAGGAAC CTGAGAGGAG AGCCATCAGC AAGCCACCAG 
     3361 CTGGAAGCAC CAAGTATTTT GGCACTGCCA AAGCCCGCTA CGACTTCTGT GCCCGGGACA 
     3421 GGTCGGAACT GTCCCTTAAG GAGGGTGATA TCATCAAGAT CCTCAATAAG AAGGGACAGC 
     3481 AAGGCTGGTG GCGTGGGGAG ATCTACGGCC GGATCGGCTG GTTCCCTTCT AACTATGTGG 
     3541 AGGAAGACTA TTCCGAATAT TGCACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3601 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     3661 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     3721 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     3781 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     3841 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     3901 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     3961 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4021 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4081 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4141 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4201 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4261 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4321 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4381 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4441 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4501 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4561 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4621 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4681 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4741 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4801 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4861 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4921 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     4981 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5041 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5101 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5161 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5221 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5281 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5341 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5401 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5461 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5521 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5581 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     5641 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     5701 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     5761 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     5821 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     5881 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     5941 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6001 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6061 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6121 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6181 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6241 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6301 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6361 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6421 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6481 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6541 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6601 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     6661 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     6721 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     6781 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     6841 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     6901 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     6961 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7021 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7081 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7141 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7201 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7261 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7321 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7381 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7441 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7501 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7561 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7621 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     7681 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     7741 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     7801 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     7861 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     7921 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     7981 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8041 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8101 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8161 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8221 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8281 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8341 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8401 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8461 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8521 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8581 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     8641 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     8701 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     8761 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     8821 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     8881 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     8941 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9001 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9061 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//