LOCUS       MG226651                9311 bp    DNA     circular  SYN 
DEFINITION  Il6st (tGFP-tagged) - Mouse interleukin 6 signal transducer (Il6st), (10ug)
ACCESSION   NM_010560
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3792..4496 
                     /label="GFP"  
     misc_feature    4543..5128 
                     /label="PolyA signal"  
     misc_feature    7220..7865 
                     /label="ColE1"  
     misc_feature    5582..6376 
                     /label="Neo"  
     misc_feature    5213..5515 
                     /label="SV40 ori"  
     misc_feature    7880..8740 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3771..3777 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3771 
                     /label="Il6st"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGCACCA AGGATTTGGC TAGCGCAAGC TTTGCTTTTT TTCCTCACCA 
     1081 CTGAATCTAT AGGTCAACTT TTGGAACCGT GTGGTTACAT CTACCCTGAA TTTCCAGTTG 
     1141 TCCAGCGCGG CTCGAACTTC ACTGCCATTT GTGTGCTGAA GGAGGCGTGT CTGCAGCATT 
     1201 ACTACGTGAA TGCCAGCTAC ATCGTGTGGA AGACCAACCA TGCTGCTGTT CCCAGGGAGC 
     1261 AGGTCACTGT CATCAACAGA ACCACGTCCA GTGTCACGTT CACAGACGTG GTCCTCCCGA 
     1321 GCGTGCAGCT CACCTGCAAC ATCCTGTCCT TTGGGCAGAT CGAGCAGAAT GTGTATGGAG 
     1381 TCACCATGCT TTCAGGCTTT CCTCCAGATA AACCTACAAA TTTGACTTGC ATTGTGAATG 
     1441 AGGGGAAGAA TATGCTGTGC CAGTGGGACC CCGGAAGGGA GACTTACCTT GAAACAAACT 
     1501 ACACTTTGAA ATCAGAGTGG GCAACAGAGA AGTTTCCTGA TTGCCAGTCA AAGCATGGCA 
     1561 CTTCATGTAT GGTCAGCTAC ATGCCCACCT ATTATGTCAA CATTGAAGTC TGGGTGGAAG 
     1621 CAGAGAATGC CCTTGGGAAG GTCTCCTCAG AGTCTATCAA TTTTGACCCC GTGGATAAAG 
     1681 TGAAACCCAC CCCACCATAT AATTTATCAG TGACCAACTC AGAAGAATTA TCCAGTATAT 
     1741 TAAAGCTATC ATGGGTCAGT TCAGGGCTGG GCGGTCTTTT AGATCTAAAG TCTGACATCC 
     1801 AATATAGGAC CAAAGATGCC TCAACTTGGA TCCAGGTCCC TCTTGAAGAT ACAATGTCTC 
     1861 CTCGAACTTC CTTCACTGTG CAGGACCTCA AGCCTTTTAC AGAATATGTG TTTAGGATCC 
     1921 GGTCCATTAA GGACAGTGGG AAGGGCTACT GGAGTGACTG GAGTGAGGAG GCTAGTGGGA 
     1981 CCACATACGA AGACAGACCA TCCAGACCAC CAAGTTTCTG GTATAAGACA AATCCATCCC 
     2041 ATGGGCAGGA ATATAGATCT GTACGGCTCA TATGGAAGGC ACTGCCTCTT TCTGAAGCCA 
     2101 ATGGGAAAAT CTTGGATTAT GAAGTGATTC TTACGCAGTC AAAGTCCGTC TCACAAACGT 
     2161 ACACAGTCAC TGGCACAGAG CTGACCGTGA ATCTCACCAA TGACCGCTAT GTCGCGTCTC 
     2221 TAGCAGCAAG AAACAAGGTG GGCAAATCAG CTGCAGCTGT CCTCACCATC CCCAGCCCCC 
     2281 ACGTCACAGC TGCTTATTCT GTAGTGAATC TTAAAGCATT TCCAAAAGAT AACCTGCTCT 
     2341 GGGTGGAATG GACACCTCCA CCTAAACCCG TGAGCAAGTA CATCTTAGAG TGGTGTGTGT 
     2401 TGTCAGAGAA CGCACCCTGT GTTGAAGACT GGCAGCAGGA AGACGCTACC GTGAATCGGA 
     2461 CCCACTTGAG AGGACGCCTC CTGGAGAGCA AGTGCTATCA AATCACAGTA ACTCCCGTAT 
     2521 TCGCCACGGG GCCCGGAGGC TCTGAGTCCT TGAAGGCGTA CCTCAAACAA GCCGCTCCTG 
     2581 CCAGAGGACC GACTGTTCGG ACAAAGAAAG TGGGGAAAAA TGAAGCTGTC TTAGCGTGGG 
     2641 ACCAGATTCC TGTGGACGAC CAGAATGGCT TCATTAGAAA CTACTCCATA TCTTACAGAA 
     2701 CCAGCGTGGG AAAGGAGATG GTTGTGCATG TGGATTCTTC TCACACGGAG TACACGCTGT 
     2761 CCTCTCTGAG TAGTGATACG TTGTACATGG TCCGAATGGC CGCGTACACA GATGAAGGTG 
     2821 GGAAAGATGG GCCGGAATTC ACTTTTACAA CACCAAAGTT CGCTCAAGGA GAAATAGAAG 
     2881 CCATAGTCGT GCCTGTGTGC TTAGCCTTCC TCCTGACAAC CCTGCTGGGC GTCTTGTTCT 
     2941 GCTTTAACAA ACGAGATCTA ATTAAAAAAC ACATCTGGCC TAATGTTCCT GATCCTTCCA 
     3001 AGAGTCATAT TGCCCAGTGG TCACCTCACA CCCCCCCAAG GCACAATTTT AACTCCAAAG 
     3061 ATCAAATGTA CTCGGACGGC AATTTCACTG ATGTAAGCGT TGTGGAAATA GAAGCAAACA 
     3121 ACAAGAAGCC TTGTCCAGAT GACCTGAAGT CCGTGGACCT GTTCAAGAAG GAGAAAGTGA 
     3181 GTACAGAAGG GCACAGCAGT GGCATCGGGG GCTCTTCATG CATGTCCTCC TCCAGGCCCA 
     3241 GCATCTCCAG CAACGAGGAG AATGAGTCTG CTCAGAGCAC CGCCAGCACG GTGCAGTACT 
     3301 CCACTGTGGT GCACAGCGGC TACAGGCACC AGGTCCCGTC CGTGCAAGTG TTCTCAAGGT 
     3361 CCGAGTCCAC CCAGCCCCTG CTAGACTCGG AGGAGCGGCC AGAAGACCTG CAGCTGGTGG 
     3421 ATAGTGTAGA CGGTGGGGAT GAGATCTTGC CCAGGCAATC GTATTTCAAG CAGAACTGCA 
     3481 GTCAGCCTGA AGCCTGTCCA GAGATTTCAC ATTTTGAAAG GTCAAACCAG GTTTTGTCCG 
     3541 GCAATGAGGA GGATTTTGTC AGACTGAAGC AGCAGCAGGT TTCAGATCAC ATTTCTCAGC 
     3601 CCTATGGATC CGAGCAACGG AGGCTGTTTC AGGAAGGCTC TACAGCGGAT GCTCTTGGCA 
     3661 CGGGGGCTGA TGGACAGATG GAGAGATTTG AATCTGTTGG AATGGAGACC ACAATTGATG 
     3721 AAGAAATTCC CAAAAGTTAC TTGCCACAGA CTGTAAGACA AGGTGGCTAC ATGCCGCAGA 
     3781 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     3841 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     3901 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     3961 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     4021 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     4081 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     4141 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     4201 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     4261 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     4321 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     4381 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4441 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4501 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4561 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4621 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4681 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4741 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4801 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4861 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4921 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4981 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5041 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5101 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5161 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     5221 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5281 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5341 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     5401 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5461 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5521 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5581 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5641 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5701 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5761 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     5821 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     5881 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     5941 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     6001 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     6061 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     6121 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     6181 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     6241 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     6301 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     6361 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6421 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6481 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6541 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6601 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6661 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6721 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6781 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     6841 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     6901 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     6961 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     7021 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     7081 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     7141 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     7201 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     7261 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     7321 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     7381 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7441 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7501 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7561 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7621 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7681 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7741 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7801 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     7861 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     7921 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     7981 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     8041 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     8101 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     8161 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     8221 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     8281 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     8341 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     8401 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8461 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8521 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8581 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8641 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8701 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8761 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     8821 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     8881 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     8941 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     9001 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     9061 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     9121 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     9181 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     9241 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     9301 ACGCGAATTT T
//