LOCUS       MG226489                9446 bp    DNA     circular  SYN 
DEFINITION  Usp4 (tGFP-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), (10ug)
ACCESSION   NM_011678
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3927..4631 
                     /label="GFP"  
     misc_feature    4678..5263 
                     /label="PolyA signal"  
     misc_feature    7355..8000 
                     /label="ColE1"  
     misc_feature    5717..6511 
                     /label="Neo"  
     misc_feature    5348..5650 
                     /label="SV40 ori"  
     misc_feature    8015..8875 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3906..3912 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3906 
                     /label="Usp4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAGGC CGGGGCAGCC GTGAGCGACC GGATGTGGAG ACCCAGAAGA 
     1081 CGGAGCTCGG AGCCTTGATG GGGACCACGC TCCAACGTGG GGCGCAGTGG TATCTTATTG 
     1141 ACAGCCGGTG GTTCAAGCAG TGGAAGAAGT ATGTGGGCTT TGACAGCTGG GACATGTACA 
     1201 ATGTCGGGGA GCATAACCTG TTTCCTGGAC CTATTGACAA CTCCGGACTC TTCTCAGATC 
     1261 CTGAGAGTCA GACCTTGAAG GAGCACTTAA TCGATGAGCT GGACTATGTG CTGGTCCCAG 
     1321 CCGAAGCCTG GAATAAATTG CTGAATTGGT ATGGCTGTGT GGAGGGCCAG CAGCCTATTG 
     1381 TCAGAAAAGT TGTGGAGCAT GGCCTGTTTG TCAAGCACTG CAAAGTGGAA GTGTATTTGC 
     1441 TGGAGCTGAA GCTCTGTGAG AACAGTGACC CCACCAATGT GCTAAGTTGC CATTTTAGCA 
     1501 AAGCAGACAC CATTGCAACT ATTGAGAAGG AGATGAGGAA GCTCTTCAAC ATCCCTGCAG 
     1561 AACGTGAAAC ACGGCTTTGG AACAAATACA TGAGCAACAC CTATGAGCAG TTGAGCAAGC 
     1621 TAGACAACAC TATCCAGGAT GCTGGGCTGT ACCAGGGTCA GGTGCTAGTA ATTGAGCCCC 
     1681 AAAATGAAGA TGGCACATGG CCCCGGCAGA GCCTGCAGTC AAAATCAAGC ACTGCACCTA 
     1741 GCAGAAATTT CACTACCTCT TCAAAACCAT CCGCAAGTCC CTATTGCTCA GTGTCTGCCT 
     1801 CTCTCATTGC AAATGGTGAT AGCACTAACA GCTCTGGGAT GCACAGCTCC GGTGTCAGCA 
     1861 GGGGTGGATC TGGCTTCTCT GCTTCGTATA ATTGCCAGGA GCCCCCATCA CCTCATATAC 
     1921 AGCCTGGCCT CTGTGGACTT GGAAACCTGG GGAACACTTG CTTCATGAAC TCTGCTCTGC 
     1981 AGTGCCTGAG CAACACTGCC CCACTGACTG AGTACTTTCT CAAAGATGAG TATGAGGCCG 
     2041 AGATCAACCG AGACAACCCT CTGGGGATGA AAGGGGAGAT TGCAGAGGCC TATGCAGAGC 
     2101 TCATCAAGCA GATGTGGTCT GGAAGGGACA CTCACGTGGC ACCACGGATG TTCAAGACGC 
     2161 AAGTGGGACG TTTTGCCCCT CAGTTTTCTG GCTACCAACA GCAAGACTCC CAGGAGCTGC 
     2221 TAGCCTTTAT TCTAGACGGA CTGCACGAGG ACCTGAACCG CGTAAAGAAG AAGCCTTACC 
     2281 TGGAGCCCAA GGACGCCAAT GGGCGACCAG ATGCGGTGGT AGCAAAGGAA GCCTGGGAAA 
     2341 ACCACAGGCT GAGGAATGAT TCTGTGATTG TGGATACTTT CCATGGCCTT TTCAAATCGA 
     2401 CTTTGGTTTG CCCAGAATGT GCTAAAGTTT CTGTGACCTT TGACCCATTT TGCTATCTAA 
     2461 CTCTCCCACT GCCTTTGAAG AAGGATCGGA TTATGGAGGT CTTCCTGGTT CCTGCTGACC 
     2521 CTCAGTGCAG ACCTATCCAG TACCGTGTGA CTGTGCCATT GATGGGGGCC ATTTCTGACC 
     2581 TGTGTGAAGC ACTCTCCAAG CTGTCTGGCA TTGCTGCAGA AAATATGGTG GTCACTGATG 
     2641 TATATAATCA CCGCTTCCAC AAAATTTTCC AAATGGATGA AGGTTTAAGC CACATCACGC 
     2701 CTCGAGATGA CATTTTTGTG TATGAGGTCT GCAACACGTC CATGGATGGC TCAGAGTGTA 
     2761 TCACTCTTCC AGTCTACTTC AGAGAGAAGA AGTCCAGGCC GTCGAGTGCT TCCTCGGGGG 
     2821 CTGTGCTCTA TGGACAGCCC CTTCTTGTCT CTGTCCCTAA GCATAAGCTA ACCCTAGAGT 
     2881 CTTTGTACCA GGCTGTTTGT GATCGTATCA GCCGCTACAT AAAACAGCCT TTGCCTGATG 
     2941 AGTTTCTCAG CTCACCCTTA GAGCCTGGGG CCTGCAATGG CTCTAGGAGT AGCTATGAAG 
     3001 GAGATGAAGA GGAAGAAATG GATCATCAAG AGGAAGGAAA AGAGCAGCTT TCCGAAGTGG 
     3061 AAGGCAGTGG TGAGGACGAT CAGGGAGATG ACCATAGCGA GAGCGCCCAA AAGGTGAAAG 
     3121 GCCAGCCGAG GCACAAGAGG CTCTTTACCT TCAGCCTCGT GAACTCCTGT GGAACTGCTG 
     3181 ATATCAATTC ACTGGCAACT GACGGGAAAC TCCTCAAACT CAACTCTCGA TCCACACTGG 
     3241 CCATTGACTG GGACAGTGAG ACCCGAAGCC TTTACTTTGA TGAGCAAGAA TCTGAGGCCT 
     3301 GTGAGAAGCA CCTGAGCATG TCACAGCCGC AGAAGAAGAA GAAGGCGGCA GTAGCCCTGC 
     3361 GAGAGTGCAT CGAGCTCTTC ACCACCATGG AGACCCTTGG GGAGCATGAC CCCTGGTACT 
     3421 GTCCCACCTG TAAGAAGCAC CAGCAGGCAA CAAAGAAGTT TGACTTGTGG TCTTTGCCCA 
     3481 AGATCCTGGT GGTTCACCTC AAGCGTTTCT CCTATAACAG ATACTGGCGG GATAAACTTG 
     3541 ACACCGTGGT GGAGTTCCCA GTCAGAGCTC TGAACATGTC CGAGTTTGTC TGTGACCGGT 
     3601 CAGCAAGGCC TTATGTTTAT GACCTAATTG CTGTGTCCAA TCACTATGGA GCCATGGGGG 
     3661 TTGGTCACTA CACTGCATAT GCGAAGAACA GACTGAACGG GAAATGGTAT TACTTTGATG 
     3721 ATAGCAGTGT GTCCCTGGCC TCTGAGGACC AGATAGTGAC GAAAGCCGCC TACGTGTTGT 
     3781 TCTATCAGCG TCGGGATGAC GAATGCTCCA GCACATCTTC GCTCGGCAGT TTCCCCGGTT 
     3841 CTGATGGAGG GGTGAAGCTG AGCAGCTCAC ACCAGGGCAT GGGGGATGAG GAGGCTTACA 
     3901 ACATGGACAC CAACACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3961 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     4021 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     4081 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     4141 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     4201 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     4261 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4321 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4381 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4441 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4501 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4561 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4621 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5341 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5401 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5461 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5521 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5581 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5641 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5701 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5761 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5821 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5881 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5941 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     6001 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     6061 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     6121 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     6181 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     6241 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6301 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6361 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6421 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6481 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6541 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6601 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6661 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6721 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6781 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6841 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6901 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6961 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     7021 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     7081 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     7141 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     7201 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7261 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7321 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7381 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7441 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7501 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7561 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7621 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7681 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7741 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7801 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7861 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7921 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7981 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     8041 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     8101 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     8161 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     8221 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     8281 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8341 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8401 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8461 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8521 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8581 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8641 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8701 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8761 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8821 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8881 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8941 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     9001 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     9061 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     9121 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     9181 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     9241 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9301 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9361 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9421 TTTAACAAAA ATTTAACGCG AATTTT
//