LOCUS       MG226447                9251 bp    DNA     circular  SYN 
DEFINITION  Usp33 (tGFP-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33) transcript variant 2, (10ug)
ACCESSION   NM_001076676
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3732..4436 
                     /label="GFP"  
     misc_feature    4483..5068 
                     /label="PolyA signal"  
     misc_feature    7160..7805 
                     /label="ColE1"  
     misc_feature    5522..6316 
                     /label="Neo"  
     misc_feature    5153..5455 
                     /label="SV40 ori"  
     misc_feature    7820..8680 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3711..3717 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3711 
                     /label="Usp33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGACTTTT CGAAATCATT GTCCACATTT GGATTCAGTT GGTGAAATAA 
     1081 CGAAAGAGGA CTTGATACAG AAATCTCTCG GCGCTTGTCA GGACTGTAAA GTCAGAGGAC 
     1141 CAAACCTGTG GGCGTGCCTG GAGAATAGGT GCTCCTATGT TGGCTGCGGG GAGTCGCAAG 
     1201 TGGACCACAG CACCATACAC TCGCAGGAGA CAAAGCATTA TCTAACTGTG AACCTCACCA 
     1261 CTCTTCGAGT ATGGTGTTAT GCTTGCAGCA AAGAAGTATT TTTGGATAGA AAATTAGGAA 
     1321 CTCCTCCTTC ATTACCTCAT GTAAGACAGC CTCAACAAAC ACAAGAAAAC AGTGTCCAGG 
     1381 ATTTTAAAAT TCCCAGTAAT CCAGCATTGA AAACCCCCAT GGTTGCTGTG TCTGAAGATC 
     1441 TGGATATAGA AGTGGAAGAG GAAGACGAGC TGAAGGCTAG AGGCCTGACA GGTTTGAAAA 
     1501 ACATTGGAAA TACTTGTTAT ATGAACGCGG CGCTGCAGGC TCTTTCTAAC TGCCCACCTT 
     1561 TGACACAGTT CTTTCTTGAT TGTGGAGGAC TGGCTAGAAC AGATAAGAAA CCAGCAATTT 
     1621 GTAAAAGTTA TCTCAAACTA ATGACCGAGT TGTGGCACAA GAGCAGGCCA GGATCTGTTG 
     1681 TGCCGGCTAA TCTGTTCCAA GGAATTAAAA CTGTAAATCC AACTTTTCGA GGTTATTCTC 
     1741 AGCAGGATGC CCAGGAATTC CTTCGCTGTC TAATGGACCT GCTTCATGAG GAGCTGAAGG 
     1801 AGCAGGTCAT GGAAATGGAG GAAGAGCCTC AAACACTAAC TTCTGAGGAG ACGGTGGAGG 
     1861 AAGAGAAGAG CCAGTCAGAT GTGGATTTTC AGTCGTGCGA GTCTTGTAGC AGCAGCGAGA 
     1921 AAGCAGAAAA TGAGAGTGGC TCCAAAGGCT TTCCTGAGGA CAGCAATGAG ACCACCATGC 
     1981 TCATCCAGGA CGAGGATGAC CTGGAGATGG CCAAAGACTG GCAGAAAGAG AAGGTGTGCA 
     2041 ATAAGATCAA CAAGGCAAAT GCCGATGTAG AACTGGACAA AGACAGGGAC ACAGTGTGTG 
     2101 AAACAGTTGA CCTAAACAGC CAGGAGACCG TCAAAGTGCA GATACACGGC AGAGCCTCAG 
     2161 AATCTATCAC TGATGTCCAT CTGAATGACC TAGCTACGTC ACAGATCCTT CCTTCAAATG 
     2221 AAAGTGTTAG TCCACGGTTA TCAGCAAGCC CTCCTAAGCT AGGCAGTCTG TGGCCAGGAC 
     2281 TGTCGCCTCC ACACAAGAAA GCTCAGTCTA CATCTGCAAA GAGGAAAAAG CAGCATAAGA 
     2341 AATACAGAAG TGTCATCTCC GACATATTCG ATGGAACAGT CATTAGCTCG GTACAGTGTC 
     2401 TGACGTGCGA TAGGGTGTCT ATAACCCTCG AGACCTTTCA GGATCTGTCC TTGCCGATTC 
     2461 CTGGCAAGGA GGACCTGGCT AAGCTGCACT CCTCCAGTCA CCCAACTATA GTCAAAGCAG 
     2521 GGTCATGTGG TGAAGCGTAC GCCCCGCAGG GGTGGATAGC TTTCTTCATG GAGTATGTGA 
     2581 AGAGCTGGTT TTGGGGTCCA GTAGTTACCT TGCAAGATTG TCTTGCTGCC TTCTTCGCCA 
     2641 GAGATGAACT TAAAGGTGAC AACATGTACA GTTGTGAAAA ATGCAAAAAG TTGAGGAATG 
     2701 GAGTAAAGTT TTGTAAAGTA CAGAAGTTTC CTGAGATTTT GTGTATCCAC CTTAAAAGAT 
     2761 TTCGACATGA ACTGATGTTT TCCACCAAAA TTAGCACCCA TGTTTCCTTC CCCCTGGAAG 
     2821 GCCTCGATCT TCAGCCATTT CTTGCAAAGG ACAGCCCAGC TCAGATTGTG ACATATGATC 
     2881 TCCTGTCAGT CATCTGTCAC CATGGGACTG CAAGTAGTGG GCACTACATT GCCTACTGCC 
     2941 GAAACAATTT AAATAACCTG TGGTATGAGT TTGATGACCA GAGCGTCACT GAAGTTTCAG 
     3001 AGTCCACTGT ACAGAATGCC GAGGCCTACG TCCTTTTCTA CAGGAAGAGC AGTGAAGAGG 
     3061 CACAAAAGGA GAGGCGGAGG ATATCAAATT TGTTGAACAT CATGGAACCT AGCCTCCTTC 
     3121 AGTTCTACAT ATCTCGACAG TGGTTGAATA AATTTAAGAC CTTTGCTGAA CCTGGCCCTA 
     3181 TTTCAAATAA TGATTTTCTC TGTATCCATG GAGGTATTCC TCCACGAAAA GCGAGTTATA 
     3241 TTGAAGACTT AGTTTTGATG CTGCCTCAGA ACATTTGGGA TAACCTCTAT AGCAGGTATG 
     3301 GAGGAGGGCC TGCTGTCAAC CATCTCTACA TCTGCCACAC CTGCCAAATT GAGTTAGAGA 
     3361 AGATTGAAAA ACGAAGAAAA ACCGAATTGG AAATTTTTAT TCGGCTCAAC AGAGCATTTC 
     3421 AAGAGGAGGA CTCCCCAGCT ACTTTTTACT GTATCAGCAT GCAGTGGTTT AGAGAATGGG 
     3481 AGAGTTTTGT AAAGGATCCC CCAGGTCCAA TCGACAACAC TAAAATTGCG GTTACTAAAT 
     3541 GTGGCAGTGT GATGCTCAAG CAAGGAGCAG ACTCTGGTCA AATTTCAGAA GAAACATGGA 
     3601 ATTTCCTGCA GTCTATATAT GGTGGGGGGC CTGAAGTTAT CCTCCGACCT CCAGTTGTTC 
     3661 ATGTTGACCC TGATGTACTC CAAGCAGAGG AAAAGATTGA AGTAGAAACT CGCTCTTTGA 
     3721 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     3781 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     3841 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     3901 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     3961 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     4021 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     4081 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     4141 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     4201 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     4261 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     4321 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4381 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4441 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4501 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4561 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4621 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4681 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4741 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4801 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4861 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4921 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4981 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5041 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5101 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     5161 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5221 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5281 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     5341 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5401 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5461 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5521 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5581 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5641 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5701 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     5761 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     5821 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     5881 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     5941 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     6001 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     6061 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     6121 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     6181 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     6241 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     6301 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6361 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6421 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6481 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6541 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6601 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6661 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6721 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     6781 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     6841 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     6901 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     6961 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     7021 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     7081 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     7141 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     7201 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     7261 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     7321 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7381 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7441 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7501 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7561 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7621 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7681 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7741 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     7801 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     7861 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     7921 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     7981 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     8041 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     8101 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     8161 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     8221 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     8281 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     8341 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8401 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8461 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8521 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8581 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8641 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8701 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     8761 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     8821 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     8881 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     8941 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     9001 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     9061 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     9121 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     9181 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     9241 ACGCGAATTT T
//