LOCUS       MG226445                9287 bp    DNA     circular  SYN 
DEFINITION  Usp33 (tGFP-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33) transcript variant 1, (10ug)
ACCESSION   NM_133247
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3768..4472 
                     /label="GFP"  
     misc_feature    4519..5104 
                     /label="PolyA signal"  
     misc_feature    7196..7841 
                     /label="ColE1"  
     misc_feature    5558..6352 
                     /label="Neo"  
     misc_feature    5189..5491 
                     /label="SV40 ori"  
     misc_feature    7856..8716 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3747..3753 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3747 
                     /label="Usp33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGACTTTT CGAAATCATT GTCCACATTT GGATTCAGTT GGTGAAATAA 
     1081 CGAAAGAGGA CTTGATACAG AAATCTCTCG GCGCTTGTCA GGACTGTAAA GTCAGAGGAC 
     1141 CAAACCTGTG GGCGTGCCTG GAGAATAGGT GCTCCTATGT TGGCTGCGGG GAGTCGCAAG 
     1201 TGGACCACAG CACCATACAC TCGCAGGAGA CAAAGCATTA TCTAACTGTG AACCTCACCA 
     1261 CTCTTCGAGT ATGGTGTTAT GCTTGCAGCA AAGAAGTATT TTTGGATAGA AAATTAGGAA 
     1321 CTCCTCCTTC ATTACCTCAT GTAAGACAGC CTCAACAAAC ACAAGAAAAC AGTGTCCAGG 
     1381 ATTTTAAAAT TCCCAGTAAT CCAGCATTGA AAACCCCCAT GGTTGCTGTG TCTGAAGATC 
     1441 TGGATATAGA AGTGGAAGAG GAAGACGAGC TGAAGGCTAG AGGCCTGACA GGTTTGAAAA 
     1501 ACATTGGAAA TACTTGTTAT ATGAACGCGG CGCTGCAGGC TCTTTCTAAC TGCCCACCTT 
     1561 TGACACAGTT CTTTCTTGAT TGTGGAGGAC TGGCTAGAAC AGATAAGAAA CCAGCAATTT 
     1621 GTAAAAGTTA TCTCAAACTA ATGACCGAGT TGTGGCACAA GAGCAGGCCA GGATCTGTTG 
     1681 TGCCGGCTAA TCTGTTCCAA GGAATTAAAA CTGTAAATCC AACTTTTCGA GGTTATTCTC 
     1741 AGCAGGATGC CCAGGAATTC CTTCGCTGTC TAATGGACCT GCTTCATGAG GAGCTGAAGG 
     1801 AGCAGGTCAT GGAAATGGAG GAAGAGCCTC AAACACTAAC TTCTGAGGAG ACGGTGGAGG 
     1861 AAGAGAAGAG CCAGTCAGAT GTGGATTTTC AGTCGTGCGA GTCTTGTAGC AGCAGCGAGA 
     1921 AAGCAGAAAA TGAGAGTGGC TCCAAAGGCT TTCCTGAGGA CAGCAATGAG ACCACCATGC 
     1981 TCATCCAGGA CGAGGATGAC CTGGAGATGG CCAAAGACTG GCAGAAAGAG AAGGTGTGCA 
     2041 ATAAGATCAA CAAGGCAAAT GCCGATGTAG AACTGGACAA AGACAGGGAC ACAGTGTGTG 
     2101 AAACAGTTGA CCTAAACAGC CAGGAGACCG TCAAAGTGCA GATACACGGC AGAGCCTCAG 
     2161 AATCTATCAC TGATGTCCAT CTGAATGACC TAGCTACGTC ACAGATCCTT CCTTCAAATG 
     2221 AAAGTGTTAG TCCACGGTTA TCAGCAAGCC CTCCTAAGCT AGGCAGTCTG TGGCCAGGAC 
     2281 TGTCGCCTCC ACACAAGAAA GCTCAGTCTA CATCTGCAAA GAGGAAAAAG CAGCATAAGA 
     2341 AATACAGAAG TGTCATCTCC GACATATTCG ATGGAACAGT CATTAGCTCG GTACAGTGTC 
     2401 TGACGTGCGA TAGGGTGTCT ATAACCCTCG AGACCTTTCA GGATCTGTCC TTGCCGATTC 
     2461 CTGGCAAGGA GGACCTGGCT AAGCTGCACT CCTCCAGTCA CCCAACTATA GTCAAAGCAG 
     2521 GGTCATGTGG TGAAGCGTAC GCCCCGCAGG GGTGGATAGC TTTCTTCATG GAGTATGTGA 
     2581 AGAGGTTTGT TGTCTCATGT GTCCCTAGCT GGTTTTGGGG TCCAGTAGTT ACCTTGCAAG 
     2641 ATTGTCTTGC TGCCTTCTTC GCCAGAGATG AACTTAAAGG TGACAACATG TACAGTTGTG 
     2701 AAAAATGCAA AAAGTTGAGG AATGGAGTAA AGTTTTGTAA AGTACAGAAG TTTCCTGAGA 
     2761 TTTTGTGTAT CCACCTTAAA AGATTTCGAC ATGAACTGAT GTTTTCCACC AAAATTAGCA 
     2821 CCCATGTTTC CTTCCCCCTG GAAGGCCTCG ATCTTCAGCC ATTTCTTGCA AAGGACAGCC 
     2881 CAGCTCAGAT TGTGACATAT GATCTCCTGT CAGTCATCTG TCACCATGGG ACTGCAAGTA 
     2941 GTGGGCACTA CATTGCCTAC TGCCGAAACA ATTTAAATAA CCTGTGGTAT GAGTTTGATG 
     3001 ACCAGAGCGT CACTGAAGTT TCAGAGTCCA CTGTACAGAA TGCCGAGGCC TACGTCCTTT 
     3061 TCTACAGGAA GAGCAGTGAA GAGGCACAAA AGGAGAGGCG GAGGATATCA AATTTGTTGA 
     3121 ACATCATGGA ACCTAGCCTC CTTCAGTTCT ACATATCTCG ACAGTGGTTG AATAAATTTA 
     3181 AGACCTTTGC TGAACCTGGC CCTATTTCAA ATAATGATTT TCTCTGTATC CATGGAGGTA 
     3241 TTCCTCCACG AAAAGCGAGT TATATTGAAG ACTTAGTTTT GATGCTGCCT CAGAACATTT 
     3301 GGGATAACCT CTATAGCAGG TATGGAGGAG GGCCTGCTGT CAACCATCTC TACATCTGCC 
     3361 ACACCTGCCA AATTGAGTTA GAGAAGATTG AAAAACGAAG AAAAACCGAA TTGGAAATTT 
     3421 TTATTCGGCT CAACAGAGCA TTTCAAGAGG AGGACTCCCC AGCTACTTTT TACTGTATCA 
     3481 GCATGCAGTG GTTTAGAGAA TGGGAGAGTT TTGTAAAGGG TAAGGATGGA GATCCCCCAG 
     3541 GTCCAATCGA CAACACTAAA ATTGCGGTTA CTAAATGTGG CAGTGTGATG CTCAAGCAAG 
     3601 GAGCAGACTC TGGTCAAATT TCAGAAGAAA CATGGAATTT CCTGCAGTCT ATATATGGTG 
     3661 GGGGGCCTGA AGTTATCCTC CGACCTCCAG TTGTTCATGT TGACCCTGAT GTACTCCAAG 
     3721 CAGAGGAAAA GATTGAAGTA GAAACTCGCT CTTTGACGCG TACGCGGCCG CTCGAGATGG 
     3781 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3841 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3901 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3961 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     4021 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     4081 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4141 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4201 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4261 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4321 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4381 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4441 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4501 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4561 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4621 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4681 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4741 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4801 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4861 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4921 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4981 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5041 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5101 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5161 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5221 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5281 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5341 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5401 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5461 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5521 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5581 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5641 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5701 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5761 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5821 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5881 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5941 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     6001 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     6061 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6121 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6181 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6241 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6301 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6361 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6421 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6481 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6541 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6601 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6661 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6721 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6781 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6841 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6901 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6961 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     7021 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     7081 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7141 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7201 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7261 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7321 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7381 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7441 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7501 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7561 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7621 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7681 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7741 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7801 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7861 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7921 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7981 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     8041 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     8101 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8161 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8221 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8281 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8341 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8401 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8461 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8521 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8581 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8641 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8701 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8761 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8821 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8881 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8941 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     9001 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     9061 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9121 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9181 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9241 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//