LOCUS       MG225852                9239 bp    DNA     circular  SYN 
DEFINITION  Nfatc4 (tGFP-tagged) - Mouse nuclear factor of activated T-cells cytoplasmic calcineurin-dependent 4 (Nfatc4) transcript variant 2, (10ug)
ACCESSION   NM_001168346
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3718..4422 
                     /label="GFP"  
     misc_feature    4469..5054 
                     /label="PolyA signal"  
     misc_feature    7146..7791 
                     /label="ColE1"  
     misc_feature    5508..6302 
                     /label="Neo"  
     misc_feature    5139..5441 
                     /label="SV40 ori"  
     misc_feature    7806..8666 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3690..3697 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3690 
                     /label="Nfatc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCCGCA AGCTGCGAGG ATGAGGAGCT GGAATTTAAG CTGGTGTTCG 
     1081 GAGAGGAAAA GGAGCCCCCC CCGCTGGGCC CTGGGGGTCC TGGGGAAGAG CTGGACTCAG 
     1141 AGGATACCCC TCCATGCTGT CGTTTGGCCC TGGGGGAACC CCTTCCTTAT GGTGCTGCCC 
     1201 CTATTGGGAT TCCCCGACCC CCACCCCCTC GGCCAGGGAT GCACTCACCA CCGCCCCGTC 
     1261 CCGCCCCTTC ACCTGGCACC TGGGAGAGCC AGCCAGCTAG GTCGGTGAGG CTGGGGGGCC 
     1321 CAGGAGGTAA TGCAGGGGGT GCTGGGGGTG GTCGTGTTCT TGAGTGTCCC AGCATCCGGA 
     1381 TTACCTCCAT CTCTCCCACG CCTGACCCGC CGACCTCGCT AGAGGACACA TCTGAAACTT 
     1441 GGGGGGACGG CTCTCCTAGA GATTACCCTC CACCAGAAGG CTTTGGGGGC TATCGAGAGG 
     1501 CTGGAGGTCA GGGCGGAGGC GCTTTCTTCA GCCCGAGCCC TGGCAGCAGC AGCCTGTCTT 
     1561 CGTGGAGCTT CTTCTCGGAC GCCTCCGACG AGGCAGCCCT GTATGCAGCG TGCGACGAGG 
     1621 TGGAGTCTGA ACTTAATGAA GCAGCCTCCC GTTTTGGCTT GAGCTCTCCG CTGCCCTCGC 
     1681 CCCGGGCTTC TCCTAGGCCA TGGACCCCGG AGGATCCCTG GAGCCTATAC GGTCCAAGCT 
     1741 CGGGAGGCCG AGCGCCAGAG GATAGCTGGC TACTCCTCAG CGCTCCTGGG CCCGTCCCAG 
     1801 CCTCCCCTCG GCCTGCTTCT CCCTGCGGCA AGCGGCGCTA TTCCAGTTCC GGAACCCCAT 
     1861 CTTCGGCCTC CCCAGCTTTG TCCCGCCGAG GCAGCCTTGG GGAAGAAGGT CCAGAGCCAC 
     1921 CTCCACCACC CCCGTTGCCT CTGGTCCGGG ACCCTAGTTC CCCTGGCCCT TTTGACTATG 
     1981 TGGGTGCTCC ACCAACCGAG AGCATCCCTC AGAAAACTCG GAGGACTTCT AGCGAGCAAG 
     2041 CAGTGGCCCT GCCTCGGTCT GAAGAGCCTC CCTCGTGCAA TGGGAAGCTA CCCTCCGGTA 
     2101 CAGAGGACTC TGTGGCTGCT CCGGGGGCTC TGCGGAAAGA GGTGGCCGGC ATGGATTACC 
     2161 TAGCAGTGCC TTCTCCCCTT GCTTGGTCCA AGGCCCGGAT TGGGGGACAC AGCCCCATCT 
     2221 TCAGGACCTC TGCCCTACCT CCCCTGGACT GGCCTTTACC CAGCCAGTAT GAGCAGCTGG 
     2281 AGCTGAGGAT CGAGGTACAG CCTAGAGCAC ACCACCGAGC TCACTATGAG ACTGAGGGCA 
     2341 GCCGGGGGGC TGTCAAGGCT GCTCCTGGAG GCCACCCTGT GGTCAAGCTC CTAGGCTACA 
     2401 GTGAGAAGCC ATTGACTCTG CAGATGTTCA TTGGCACTGC AGATGAGAGG AGCCTGCGGC 
     2461 CCCATGCCTT CTACCAGGTG CACCGGATTA CTGGCAAGAT GGTGGCTACA GCCAGCTATG 
     2521 AAGCTGTAGT CAGTGGTACC AAAGTGTTGG AGATGACCTT ACTCCCAGAG AACAACATGG 
     2581 CTGCCAACAT TGACTGTGCT GGAATCCTGA AGCTTCGAAA TTCAGACATT GAGCTACGGA 
     2641 AGGGTGAGAC AGACATCGGG CGCAAAAACA CGCGTGTGCG GCTGGTGTTC CGTGTGCACG 
     2701 TGCCTCAGGG CGGCGGGAAG GTCGTGTCTG TGCAGGCAGC ATCAGTGCCC ATCGAGTGCT 
     2761 CCCAGCGCTC GGCCCAGGAG CTGCCCCAGG TGGAGACCTA CAGCCCCAGT GCCTGCTCTG 
     2821 TGAGAGGAGG GGAGGAGCTA GTGTTGACTG GTTCCAACTT CCTGCCAGAC TCTAAAGTGG 
     2881 TGTTCATTGA GAGGGGTCCT GATGGAAAAC TGCAGTGGGA GGAAGAGGCA GCGGTGAACC 
     2941 GACTGCAGAG CAGTGAGGTG ACATTGACTC TCACCATCCC CGAGTACAGC AACAAGCGGG 
     3001 TGTCCCGACC AGTCCAGGTC TACTTTTACG TCTCCAATGG ACGGAGGAAG CGCAGTCCTA 
     3061 CCCAAAGTTT CAAGTTCCTA CCTGTGGTCT TCAAGGAGGA GCCCCTACCA GACTCATCTC 
     3121 TCCGGGGCTT CCCTTCCACA TCGGGTCCCC CCTTTGGCCC TGACGTGGAC TTCTCACCGC 
     3181 CCAGGCCCCC TTACCCCTCC TATCCCCATG AAGACCCTGC TTATGAGACT CCTTATCTGT 
     3241 CAGAAGGCTT TGGTTATAGC ACACCCGCTC TGTACCCCCA GACGGGGCCC CCACCGTCCT 
     3301 ATAGATCTGG CCTGCGGATG TTCCCTGAGA CTGGGGGTAC CACAGGTTGT GCCCGCCTAC 
     3361 CTTCAGTCTC TTTCCTTCCG CGCCCCTTTC CTGGTGATCA ATATGGAGGA CAGGGCTCCT 
     3421 CTTTTGCCCT GGGGCTTCCA TTCTCCCCTC CAGCCCCCTT TAGGCCGCCA CTGCCTTCCT 
     3481 CCCCACCACT CGAAGACCCC TTCCATCCCC AGAGTGCCAT CCACCCCTTA CCTCCTGAGG 
     3541 GCTACAATGA GGTGGGGCCA GGCTATACCC CTGGGGAGGG GGCTTCGGAG CAGGAGAAAG 
     3601 CCAGGGGTGG CTACAGCAGC GGCTTCAGAG ACAGTGTACC TATCCAGGGT ATCACCCTGG 
     3661 AGGAAGGTGG GTGTGGGACT GGGGGCTGCG AGTGTAAGAG CGGACCGACG CGTACGCGGC 
     3721 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3781 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3841 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3901 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     3961 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     4021 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4081 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4141 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4201 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4261 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4321 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4381 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4441 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4501 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4561 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4621 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4681 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4741 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4801 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4861 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4921 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4981 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5041 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5101 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5161 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5221 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5281 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5341 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5401 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5461 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5521 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5581 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5641 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5701 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5761 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5821 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5881 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     5941 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     6001 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6061 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6121 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6181 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6241 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6301 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6361 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6421 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6481 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6541 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6601 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6661 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6721 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6781 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6841 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6901 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     6961 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     7021 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7081 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7141 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7201 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7261 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7321 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7381 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7441 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7501 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7561 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7621 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7681 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7741 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7801 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7861 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7921 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     7981 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     8041 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8101 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8161 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8221 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8281 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8341 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8401 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8461 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8521 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8581 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8641 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8701 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8761 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8821 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8881 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     8941 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     9001 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9061 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9121 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9181 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//