LOCUS       MG225812                9479 bp    DNA     circular  SYN 
DEFINITION  Pcdh8 (tGFP-tagged) - Mouse protocadherin 8 (Pcdh8) transcript variant 2, (10ug)
ACCESSION   NM_001042726
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3960..4664 
                     /label="GFP"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    7388..8033 
                     /label="ColE1"  
     misc_feature    5750..6544 
                     /label="Neo"  
     misc_feature    5381..5683 
                     /label="SV40 ori"  
     misc_feature    8048..8908 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3939..3945 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3939 
                     /label="Pcdh8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTCCTGCG AAGCGCTGGG GCAGCCCTTG CCTTTTCCCC TTGCAGCTAT 
     1081 TTAGTCTCTG CTGGGTGCTC TCAGTGGCCC AGAGCAAGAC AGTCCGATAC AGCACGTTCG 
     1141 AAGAGGATGC CCCTGGCACA GTCATCGGAA CCCTTGCAGA AGACCTGCAC ATGAAAGTGT 
     1201 CTGGAGACAC AAGCTTCCGC CTGATGAAGC AATTCAACAG CTCTCTGCTC CGGGTACGCG 
     1261 AGGGCGACGG GCAGCTGACC GTCGGGGATG CGGGCCTGGA CCGTGAGCGT CTGTGTGGCC 
     1321 CGTCCCCACA GTGCGTGCTG GCCTTCGATG TTGTCAGCTT CTCGCAGGAA CAGTTCCGCC 
     1381 TGGTGCACGT GGAGGTGGAG GTGAGGGATG TAAACGACCA CGCGCCCCGC TTTCCCCGAG 
     1441 CCCAGATCCC GGTGGAGGTC TCAGAGAGCG CTCCGGTAGG CACACGCATC CCCCTGGAGG 
     1501 TGCCCGTGGA CGAGGACGTG GGTGCCAACG GGCTGCAGAG TGTACGCCTA GCCGAGCCTC 
     1561 ACAGCCCCTT CCGCGTGGAG CTGCAGACGC GCGCGGACGG TGCCCAGTGT GCGGACCTGG 
     1621 TGCTGCTACA GGAGTTGGAC CGCGAGAGCC AGGCCTCCTA CAGCCTGGAG CTGGTGGCTC 
     1681 AGGATGGCGG GCGACCGCCG CGTTCCGCCA CAGCAGCCCT CAGCGTGCGT GTGCTAGATG 
     1741 CCAATGACCA CAGCCCGGCC TTCCCACAGG GTGCCGTGGC AGAGGTGGAA TTGGCCGAGG 
     1801 ACGCACCTGT GGGATCGCTG CTGCTCGACC TGGACGCTGC CGATCCCGAC GAAGGTCCCA 
     1861 ACGGCGACGT TGTGTTCACC TTCGGCGCCC GCACCCCTCC CGAAGCCCGC CACCTCTTCC 
     1921 GGCTCGACCC GCGCTCTGGC CGCCTCACCT TGGCTGGGCA GGTGGACTAC GAGCGCCAGG 
     1981 ACACCTACGA GCTGGACGTG CGGGCTCAAG ACCGAGGTCC AGGGCCCCGG ACAGCCACCT 
     2041 GCAAGGTTAT CGTGCGCATC CGAGACGTCA ACGACAACGC TCCTGAAATT TCTATCACTC 
     2101 CTCTGGCCGC CCCGGGGGCT CCAGCCACCT CGCCCTTCGC CGCCGCCGCC GCTGCCGCCG 
     2161 CACTCGGAGG AGCGGACGCG GCCTCGTCGA CTGGGTCCGG GACACAGGAA GCCGGTATCA 
     2221 CCTCGCTGGT GCCAGAGGGG GCGGCGCGCG AGAGTCTAGT GGCGCTGGTC AGCACCTCGG 
     2281 ACAGGGACTC GGGCGCCAAC GGGCAGGTGC GCTGCGCCCT CTACGGGCAC GAGCACTTTA 
     2341 GGCTGCAGCC GGCCTATGCT GGCAGCTACC TGGTGGTGAC CGCTGCATCT CTGGACCGGG 
     2401 AGCGCATCGC GGAGTACAAC CTGACGCTGG TGGCCGAAGA CCGTGGCACA CCCCCTCTTC 
     2461 GCACCGTCAG GCCCTACACC GTGCGAGTGG GGGACGAGAA CGACAACGCA CCGATCTTCA 
     2521 CCAAGCCAGT CTATGAGGTG TCGGTCCGCG AAAACAACCC TCCAGGCGCC TACCTGGCCA 
     2581 CAGTGGCAGC CCGCGACCCT GACGTGGGTC GCAACGGTCA AGTCACCTAC CGCCTGGTAG 
     2641 AGGCCGAAGT GGGCCGCTCT GGGGAAGCCG TGTCCACTTA CGTCTCAGTA GACCCGGCTA 
     2701 CCGGAGCCAT CTACGCTCTC CGTAGTTTTG ACTATGAGAC TCTGCGCCAG CTTGACGTGC 
     2761 GTGTCCAGGC CAGCGACGGC GGTTCCCCTC AGCTTTCCAG CAATGCTCTG GTGCAAGTGC 
     2821 GGGTGCTGGA CCAGAATGAT CATTCTCCGA TCCTTGTGCA TCCGGCGCCG GCTAATGGCT 
     2881 CCCTAGAAGT AGCGGTCCCT GGACGTTCCA CCAAGGACAC AGCTGTGGCG CGCATCCAGG 
     2941 CCCGGGATGC TGACGAAGGA GCCAACGGGG AACTGGCCTT TGATCTGCTG CAGCAGGAGC 
     3001 CACGCGAGGC CTTCTCCATA GGCCGCCACA CGGGGGAGAT CATGCTTACT GGAGACCTCT 
     3061 CGCAGGAGCC TCCTGGCCGC GTGTTCAAGG CCCTGCTGGT CATATCCGAC GGCGGCCGTC 
     3121 CCCCTCTCAC CACCACTGCA ACCGTCAGTT TCGTGGTAAC AGCCGGGGGC GGGTCAGCTG 
     3181 TGCCTGCCAG CTCCGGGAGC CCAGAGCACT CCCGGCCACC CGGCTCTCGC CTCGCGCCGT 
     3241 CGGGGCCTTC GCTGCAGTGG GACACGCCGC TGATCGTCAT CATCGTGTTA GCCGGGAGCT 
     3301 GCACGCTGCT GCTTGCAGCC ATCATCGCCA TCGCCACCAC CTGCAACCGC CGCAAGAAGG 
     3361 AGCCTTACGG TGCCTCTCCA GGCTTCGGGA AGGAGCCTGC TGCGCCCCCT GTTGCAGTCT 
     3421 GGAAGGGTCA TTCATTCAAC ACCATCTCGG GCCGAGAAGC TGAGAAGTTC AGTGGGAAAG 
     3481 ACAGCGGCAA AGGAGACAGT GATTTCAATG ACAGTGACTC GGACATCAGC GGGGACGCTT 
     3541 TGAAAAAAGA CCTCATCAAC CACATGCAGA GTGGACTGTG GGCGTGTACT GCTGAGTGCA 
     3601 AGATCCTAGG TCATTCTGAT CGCTGCTGGA GCCCATCCTG TGCCGGACCC AACGTACACC 
     3661 CCCCTCCTCA CCCACCAGCC CAGATGTCAA CCTTCTGTAA GAGCACGTCC CTGCCTCGGG 
     3721 ATCCTCTGCG CAGGGACAAT TACTATCAGG CCCAGCTCCC CAAGACAGTA GGGTTGCAGA 
     3781 GCGTCTATGA GAAAGTACTG CATAGAGACT ATGACAGGAC AGTCACTTTA CTCTCCCCTC 
     3841 CCCGTCCAGG AAGGCTCCCA GACCTGCAGG AGATTGGAGT ACCCCTCTAT GAATCCCCTC 
     3901 CTGGTAGCAG ATACGTGTCC CCGAAGAAGG GAATCAATGA AAATGTGACG CGTACGCGGC 
     3961 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     4021 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     4081 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     4141 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     4201 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     4261 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4321 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4381 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4441 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4501 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4561 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4621 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4681 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4741 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4801 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4861 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4921 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4981 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5041 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5101 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5161 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5221 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5281 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5341 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5401 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5461 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5521 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5581 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5641 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5701 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5761 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5821 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5881 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5941 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     6001 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     6061 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     6121 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     6181 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     6241 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6301 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6361 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6421 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6481 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6541 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6601 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6661 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6721 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6781 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6841 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6901 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6961 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     7021 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     7081 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     7141 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     7201 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     7261 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7321 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7381 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7441 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7501 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7561 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7621 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7681 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7741 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7801 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7861 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7921 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7981 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     8041 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     8101 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     8161 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     8221 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     8281 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8341 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8401 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8461 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8521 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8581 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8641 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8701 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8761 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8821 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8881 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8941 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     9001 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     9061 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     9121 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     9181 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     9241 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9301 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9361 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9421 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//