LOCUS       MG225615                9161 bp    DNA     circular  SYN 
DEFINITION  Prom1 (tGFP-tagged) - Mouse prominin 1 (Prom1) transcript variant 2, (10ug)
ACCESSION   NM_001163577
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3642..4346 
                     /label="GFP"  
     misc_feature    4393..4978 
                     /label="PolyA signal"  
     misc_feature    7070..7715 
                     /label="ColE1"  
     misc_feature    5432..6226 
                     /label="Neo"  
     misc_feature    5063..5365 
                     /label="SV40 ori"  
     misc_feature    7730..8590 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3621..3627 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3621 
                     /label="Prom1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTCGTC TTCAGTGCCC TGCTGTTACT GGGGCTGTGT GGAAAGATCT 
     1081 CTTCAGAAGG TCAGCCTGCA TTCCATAACA CTCCTGGGGC TATGAATTAT GAATTGCCTA 
     1141 CCACCAAATA TGAGACCCAA GATACCTTCA ATGCTGGGAT TGTTGGCCCT CTCTACAAAA 
     1201 TGGTGCACAT CTTCCTCAAC GTGGTCCAGC CGAATGACTT CCCTCTAGAT TTGATCAAAA 
     1261 AACTCATACA GAACAAGAAC TTTGACATCT CAGTTGATTC CAAGGAGCCA GAAATCATAG 
     1321 TCTTGGCTCT GAAGATTGCC CTCTATGAGA TCGGAGTCCT TATCTGCGCC ATCCTGGGAC 
     1381 TGCTGTTCAT TATCCTCATG CCTCTGGTGG GCTGCTTCTT TTGTATGTGC CGTTGCTGCA 
     1441 ACAAATGCGG CGGAGAGATG CACCAGCGGC AGAAGCAGAA TGCGCCATGC AGGAGGAAGT 
     1501 GCTTGGGCCT CTCCCTCCTG GTGATTTGTC TGCTCATGAG CCTTGGCATT ATATATGGCT 
     1561 TTGTGGCTAA CCAGCAGACC AGGACTCGGA TCAAAGGGAC CCAGAAACTG GCAAAGAGCA 
     1621 ATTTCAGAGA CTTTCAAACA CTCCTGACTG AAACACCAAA GCAAATTGAC TATGTAGTGG 
     1681 AGCAGTACAC CAACACCAAG AACAAGGCAT TCTCAGACCT GGATGGCATC GGCTCCGTGC 
     1741 TGGGAGGCAG AATAAAGGAC CAACTAAAAC CCAAAGTAAC TCCTGTCCTC GAAGAGATTA 
     1801 AGGCCATGGC GACAGCCATC AAACAGACCA AGGATGCCCT GCAGAACATG AGCAGCAGCC 
     1861 TGAAAAGTCT CCAAGATGCA GCCACCCAGC TCAATACCAA CCTGAGCTCT GTGAGAAACA 
     1921 GCATCGAGAA TTCGCTCAGC AGCAGTGACT GTACCTCAGA TCCAGCCAGC AAGATCTGCG 
     1981 ATAGCATCAG ACCAAGCCTA AGCAGTCTGG GGAGCAGCCT CAATTCAAGT CAGCTCCCAT 
     2041 CAGTGGATAG AGAACTCAAC ACTGTTACTG AAGTCGACAA AACTGATCTG GAGAGCCTCG 
     2101 TCAAAAGGGG GTATACGACA ATTGATGAAA TACCCAATAC AATACAAAAC CAAACTGTGG 
     2161 ATGTCATCAA AGACGTCAAA AATACCTTGG ACTCCATTAG CTCCAACATT AAGGACATGA 
     2221 GCCAAAGTAT TCCTATTGAG GATATGCTGT TACAGGTCTC CCATTACCTT AATAACAGCA 
     2281 ACAGATACTT AAACCAGGAG CTGCCCAAGC TGGAAGAATA TGACTCGTAC TGGTGGCTGG 
     2341 GTGGCTTGAT TGTCTGCTTT CTGCTGACTC TCATTGTGAC CTTCTTTTTC CTGGGCTTGC 
     2401 TGTGTGGTGT GTTTGGCTAT GACAAGCATG CCACCCCAAC TAGAAGAGGC TGTGTGTCCA 
     2461 ACACTGGAGG CATCTTCCTC ATGGCTGGGG TTGGATTCGG CTTCCTTTTT TGCTGGATAT 
     2521 TGATGATCCT TGTGGTTCTT ACGTTTGTTG TTGGTGCAAA TGTGGAAAAG TTGCTCTGCG 
     2581 AACCTTATGA AAACAAGAAA TTATTACAGG TTTTGGACAC TCCCTATCTG CTCAAGGAAC 
     2641 AATGGCAATT TTATCTTTCT GGCATGCTAT TCAATAACCC AGACATTAAC ATGACCTTTG 
     2701 AGCAAGTCTA CAGGGATTGC AAAAGAGGTC GAGGTATATA TGCTGCTTTT CAGCTTGAGA 
     2761 ATGTCGTCAA CGTCAGTGAT CATTTCAACA TTGACCAGAT TTCTGAAAAC ATAAATACGG 
     2821 AGTTGGAAAA CCTGAATGTG AACATTGATA GCATTGAACT GTTGGATAAC ACAGGAAGGA 
     2881 AGAGCCTCGA GGACTTTGCA CATTCTGGGA TAGATACAAT CGATTATTCC ACATACTTGA 
     2941 AGGAGACTGA GAAATCCCCT ACTGAAGTGA ATCTGCTGAC ATTTGCCTCT ACCCTGGAAG 
     3001 CAAAAGCAAA CCAGTTGCCT GAAGGAAAGC TGAAACAGGC CTTCTTACTG GATGTACAGA 
     3061 ATATAAGAGC CATCCACCAG CATCTCCTCC CTCCTGTGCA GCAATCACTG AATACGTTAA 
     3121 GACAAAGTGT CTGGACCCTC CAGCAAACAA GCAACAAGTT GCCGGAGAAA GTGAAGAAGA 
     3181 TCCTTGCCTC TTTGGACTCT GTTCAGCATT TCCTCACCAA TAACGTTTCC CTCATCGTTA 
     3241 TCGGGGAAAC GAAGAAGTTT GGGAAAACAA TACTAGGCTA CTTTGAACAT TATCTGCACT 
     3301 GGGTCTTTTA TGCCATCACA GAGAAGATGA CATCCTGCAA ACCCATGGCC ACCGCGATGG 
     3361 ACTCTGCTGT TAATGGCATT CTGTGTGGCT ATGTTGCGGA CCCTCTGAAT TTGTTCTGGT 
     3421 TCGGCATAGG GAAAGCCACG GTGCTCTTAC TTCCGGCTGT AATCATTGCT ATCAAGCTGG 
     3481 CCAAGTACTA TCGCAGGATG GATTCAGAGG ATGTATACGA CGATGTTGAG ACTGTGCCCA 
     3541 TGAAAAATTT GGAAATCGGT AGTAATGGTT ATCATAAAGA TCATTTATAT GGTGTTCACA 
     3601 ATCCTGTTAT GACAAGCCCG TCTCGATACA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3661 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3721 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3781 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3841 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3901 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3961 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4021 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4081 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4141 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4201 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4261 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4321 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4381 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4441 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4501 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4561 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4621 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4681 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4741 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4801 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4861 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4921 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4981 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5041 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5101 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5161 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5221 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5281 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5341 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5401 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5461 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5521 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5581 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5641 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5701 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5761 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5821 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5881 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5941 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6001 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6061 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6121 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6181 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6241 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6301 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6361 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6421 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6481 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6541 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6601 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6661 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6721 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6781 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6841 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6901 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6961 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7021 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7081 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7141 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7201 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7261 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7321 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7381 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7441 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7501 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7561 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7621 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7681 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7741 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7801 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7861 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7921 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7981 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8041 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8101 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8161 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8221 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8281 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8341 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8401 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8461 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8521 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8581 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8641 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8701 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8761 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8821 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8881 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8941 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9001 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9061 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9121 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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